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The map label for this gene is emrB [H]

Identifier: 182678990

GI number: 182678990

Start: 2314934

End: 2316514

Strand: Reverse

Name: emrB [H]

Synonym: Bind_2025

Alternate gene names: 182678990

Gene position: 2316514-2314934 (Counterclockwise)

Preceding gene: 182678991

Following gene: 182678989

Centisome position: 55.55

GC content: 54.97

Gene sequence:

>1581_bases
ATGAGTGCGGTGGTGGATTCAAACGACCACGCGGACTGGAGGCCATCGCATAATCCGTGGCTGATCGCGGTCGTCGTCAC
GCTCGCCGCCTTCATGGAAATTCTCGACACGACGATCGTCAATGTCGCGCTTCCGCATATTGCCGGAGCCTTGTCCTCGT
CGAACGATGAAGCGACCTGGGCTTTGACCTCTTATCTCGTCGCCAATGGCATTGTGCTGACGATTTCCGGCTGGCTCAGC
GATGTCATTGGCCGCAAGCGTTATTTTGTTATCTGCATCATCATGTTCACGGTCTGCTCGTTTCTCTGCGGGGTCGCGAC
GAGCCTTGGACAACTCATCGTCTTTCGTCTTTTGCAGGGCTTCTTCGGCGGTGGTTTACAGCCGAACCAGCAATCGATCA
TCCTCGATACCTTCCCACCAGCAAAGCGTGGTGCGGCGTTCGGTGTTACCGCGATCGCGACGATCGTCGCGCCGGTGCTG
GGGCCGACGCTTGGCGGTTGGCTGACCGATACTTACAATTGGCGCTGGGTCTTTCTCGTCAATATACCGTTCGGCATTAT
CGCGGTCTTTCTCGTCTCTGTCCTCGTCGAAGACCCGCCGTGGGTAAAGGCGCGGCGGAGCAAGGGTATTGATTATATCG
GCTTGACTTTGATCACCGTCGGTCTCGGCTGCCTGCAGGTGATGATGGATCGCGGCGAAGATGAAGATTGGTTCAGTTCC
CATTTTATTGTCATCATGGCTCTGTTCGCGGGACTTGGCATCCTCGGCGCCATAGGTTGGCTGCTCGTCGCGAAAAAACC
GATTGTCAGTCTGGAGGTCTTCAAGGACCGTAATTATGCGGTGGGCAGTATCTGCATCGCGGCGGTTGGTGGAGTGCTTT
ATGCTGGCGCGGTCATCATTCCGCAATTTTCACAGACCGTCATCGGGTACGATGCGACCTGGTCGGGCCTCATCCTGTCG
CCAGGCGGCGTTGCTATTATCATCCTTATCCCGCTTGTCGGGCGGTTGATGAGCTTCGTCCAGACGCGTTATCTTATCAT
TGTCGGCTTTATGATCATGGGGGCCGCCTTTCTTTATTCCAGCACGCTCGTTCCGACGATCGATTTCAAGACTCTTGTGC
TGATGCGGTCGTTTCAGACCATGGGGCTCGGTTTTCTCTTTGTGCCGATCAGCACAATCGCTTATTCGACCCTGCCGCGT
GACTTGAATGGTGATGGGACAGCGCTCTTCACCATGTTGCGGAATGTCTTCGGCTCGATCGGTATTTCGCTGGCGACGGC
AATGATTACCGAACGGACACAGGTCCGCCAATCCTATCTATCGCAATGGATGACGCCGTTTTATCAGCCTTTCAACGATC
TCGTTGCTCAATATGAGCGGACCTTGATCTCGATGGGTCACGCCGCTGGAGCTGTGCATGATATTGCCTTGGGACGTGTC
TATCAGACGTTTCGCACGCAAGCACAAGTCTTGGCCTATTCGGATGTTTTTCTCTACATTGCCGTTATCGCTTTTGCCGT
CATCCCGTTCTGTTTCCTGTTTTCAAACGCGAAGGGCGGCGGCGGCGGTCCCGTGCATTAG

Upstream 100 bases:

>100_bases
TCGTCCAGCGTGTCCCGGTCAAGATCCTAATCGATAAGGGGCTTGATCCGGAGCAGCCTTTGCCGCTGGGTCTTTCGGTC
GTGCCGACGGTCACTTTGAA

Downstream 100 bases:

>100_bases
AGCATGAGACATGGGATAAAAGTCAGATATCATGCGTCGGATCATGGTGCCGATACATAAAACTGATAATATCGGTGATC
TATACTCTGTTTCTCGATTG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSAVVDSNDHADWRPSHNPWLIAVVVTLAAFMEILDTTIVNVALPHIAGALSSSNDEATWALTSYLVANGIVLTISGWLS
DVIGRKRYFVICIIMFTVCSFLCGVATSLGQLIVFRLLQGFFGGGLQPNQQSIILDTFPPAKRGAAFGVTAIATIVAPVL
GPTLGGWLTDTYNWRWVFLVNIPFGIIAVFLVSVLVEDPPWVKARRSKGIDYIGLTLITVGLGCLQVMMDRGEDEDWFSS
HFIVIMALFAGLGILGAIGWLLVAKKPIVSLEVFKDRNYAVGSICIAAVGGVLYAGAVIIPQFSQTVIGYDATWSGLILS
PGGVAIIILIPLVGRLMSFVQTRYLIIVGFMIMGAAFLYSSTLVPTIDFKTLVLMRSFQTMGLGFLFVPISTIAYSTLPR
DLNGDGTALFTMLRNVFGSIGISLATAMITERTQVRQSYLSQWMTPFYQPFNDLVAQYERTLISMGHAAGAVHDIALGRV
YQTFRTQAQVLAYSDVFLYIAVIAFAVIPFCFLFSNAKGGGGGPVH

Sequences:

>Translated_526_residues
MSAVVDSNDHADWRPSHNPWLIAVVVTLAAFMEILDTTIVNVALPHIAGALSSSNDEATWALTSYLVANGIVLTISGWLS
DVIGRKRYFVICIIMFTVCSFLCGVATSLGQLIVFRLLQGFFGGGLQPNQQSIILDTFPPAKRGAAFGVTAIATIVAPVL
GPTLGGWLTDTYNWRWVFLVNIPFGIIAVFLVSVLVEDPPWVKARRSKGIDYIGLTLITVGLGCLQVMMDRGEDEDWFSS
HFIVIMALFAGLGILGAIGWLLVAKKPIVSLEVFKDRNYAVGSICIAAVGGVLYAGAVIIPQFSQTVIGYDATWSGLILS
PGGVAIIILIPLVGRLMSFVQTRYLIIVGFMIMGAAFLYSSTLVPTIDFKTLVLMRSFQTMGLGFLFVPISTIAYSTLPR
DLNGDGTALFTMLRNVFGSIGISLATAMITERTQVRQSYLSQWMTPFYQPFNDLVAQYERTLISMGHAAGAVHDIALGRV
YQTFRTQAQVLAYSDVFLYIAVIAFAVIPFCFLFSNAKGGGGGPVH
>Mature_525_residues
SAVVDSNDHADWRPSHNPWLIAVVVTLAAFMEILDTTIVNVALPHIAGALSSSNDEATWALTSYLVANGIVLTISGWLSD
VIGRKRYFVICIIMFTVCSFLCGVATSLGQLIVFRLLQGFFGGGLQPNQQSIILDTFPPAKRGAAFGVTAIATIVAPVLG
PTLGGWLTDTYNWRWVFLVNIPFGIIAVFLVSVLVEDPPWVKARRSKGIDYIGLTLITVGLGCLQVMMDRGEDEDWFSSH
FIVIMALFAGLGILGAIGWLLVAKKPIVSLEVFKDRNYAVGSICIAAVGGVLYAGAVIIPQFSQTVIGYDATWSGLILSP
GGVAIIILIPLVGRLMSFVQTRYLIIVGFMIMGAAFLYSSTLVPTIDFKTLVLMRSFQTMGLGFLFVPISTIAYSTLPRD
LNGDGTALFTMLRNVFGSIGISLATAMITERTQVRQSYLSQWMTPFYQPFNDLVAQYERTLISMGHAAGAVHDIALGRVY
QTFRTQAQVLAYSDVFLYIAVIAFAVIPFCFLFSNAKGGGGGPVH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=478, Percent_Identity=35.3556485355649, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788710, Length=512, Percent_Identity=33.203125, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1790195, Length=418, Percent_Identity=28.2296650717703, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=24.0740740740741, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788392, Length=438, Percent_Identity=27.8538812785388, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87082320, Length=200, Percent_Identity=23.5, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=158, Percent_Identity=25.3164556962025, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=182, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=31.3725490196078, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=182, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56994; Mature: 56863

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00092 N6_MTASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVVDSNDHADWRPSHNPWLIAVVVTLAAFMEILDTTIVNVALPHIAGALSSSNDEATW
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ALTSYLVANGIVLTISGWLSDVIGRKRYFVICIIMFTVCSFLCGVATSLGQLIVFRLLQG
HHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFGGGLQPNQQSIILDTFPPAKRGAAFGVTAIATIVAPVLGPTLGGWLTDTYNWRWVFLV
HHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
NIPFGIIAVFLVSVLVEDPPWVKARRSKGIDYIGLTLITVGLGCLQVMMDRGEDEDWFSS
ECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HFIVIMALFAGLGILGAIGWLLVAKKPIVSLEVFKDRNYAVGSICIAAVGGVLYAGAVII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHEE
PQFSQTVIGYDATWSGLILSPGGVAIIILIPLVGRLMSFVQTRYLIIVGFMIMGAAFLYS
CCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLVPTIDFKTLVLMRSFQTMGLGFLFVPISTIAYSTLPRDLNGDGTALFTMLRNVFGSI
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GISLATAMITERTQVRQSYLSQWMTPFYQPFNDLVAQYERTLISMGHAAGAVHDIALGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQTFRTQAQVLAYSDVFLYIAVIAFAVIPFCFLFSNAKGGGGGPVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAVVDSNDHADWRPSHNPWLIAVVVTLAAFMEILDTTIVNVALPHIAGALSSSNDEATW
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ALTSYLVANGIVLTISGWLSDVIGRKRYFVICIIMFTVCSFLCGVATSLGQLIVFRLLQG
HHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFGGGLQPNQQSIILDTFPPAKRGAAFGVTAIATIVAPVLGPTLGGWLTDTYNWRWVFLV
HHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
NIPFGIIAVFLVSVLVEDPPWVKARRSKGIDYIGLTLITVGLGCLQVMMDRGEDEDWFSS
ECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HFIVIMALFAGLGILGAIGWLLVAKKPIVSLEVFKDRNYAVGSICIAAVGGVLYAGAVII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHEE
PQFSQTVIGYDATWSGLILSPGGVAIIILIPLVGRLMSFVQTRYLIIVGFMIMGAAFLYS
CCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLVPTIDFKTLVLMRSFQTMGLGFLFVPISTIAYSTLPRDLNGDGTALFTMLRNVFGSI
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GISLATAMITERTQVRQSYLSQWMTPFYQPFNDLVAQYERTLISMGHAAGAVHDIALGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQTFRTQAQVLAYSDVFLYIAVIAFAVIPFCFLFSNAKGGGGGPVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]