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The map label for this gene is trpA

Identifier: 182678954

GI number: 182678954

Start: 2272110

End: 2272943

Strand: Reverse

Name: trpA

Synonym: Bind_1988

Alternate gene names: 182678954

Gene position: 2272943-2272110 (Counterclockwise)

Preceding gene: 182678955

Following gene: 182678953

Centisome position: 54.51

GC content: 61.87

Gene sequence:

>834_bases
ATGCCCACACGCATCGATCGCCGCTTCGAGGAATTGGCCAAGGCCGGCCGTGCCGGGCTTGTGACTTTCGTGATGGCCGG
CGATCCGGATCTGTCGACTTCTCTCGAGATTTTGCACGCCCTGGTGCGGGCGGGTGCGGATGTGCTCGAGGTCGGCATGC
CTTTCACCGATCCCATGGCTGATGGGCCGGCGATCCAGGCGGCGGGCTTGCGGGCGCTCAAGGCTGGGATGACCTTGCGC
AAGACCCTCGACATGGTCGCGGCTTTCCGCGCGAAGGACGCGACAACCCCAATCATTTTGATGGGCTATTATAATCCGAT
CTATATCTTCGGGGTGGAGGCTTTCCTGAACCGGGCCAAGGAAGTCGGCGTGGATGGTCTCATCGTCGTCGATCTGCCTC
CCGAGGAGGATGAGGAACTATGCCTTCCGGCGCGGGCGGCCGGGCTGAATTTTATCCGGCTTGCCACACCAACGACCGAT
GCTGTGCGCCTGCCTGCCGTGCTCGCCAATACGTCGGGTTTCCTCTATTATGTGTCGATCACTGGGATCACAGGCGCAGG
GGTCCCTGATTACAGCAAGGTTGCTTCCGCGGTTGCCGGCATCAAGGCGCATACGAATCTACCAGTTGCCGTTGGTTTCG
GCGTCAAGACGGCGGAAAGCGCGCGTACGATCGCGTGCCATGCCGATGCCGTTGTCGTGGGGTCAGCCATTGTCGATGCT
TTGCGAGCCTCGCTCGATGGCGAGGGCAGGGCGACACCGGCCTGCATAGGCACTGTGGAAAAGCTTGTGGCGGAACTCGC
CGAAGGTGTGCGGAGCGCAGCAGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
TGGTTTTAACGACAAACAGCAAACTCCCAATTCGTGCCTGGAGGGACAAGGGGGAGAAATCTGTCGCACGAGAGTGCCTG
TTCAGAGGTGAATCCATTTC

Downstream 100 bases:

>100_bases
CTGCGCGCACAAAATTGAGTCGCTCCGAGAGCGTAACCCGAGACAATCGCCGAGAGAACCGCAATGAATTGGATTTCCAA
TGTCGTTCCGCCGAAAATCA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MPTRIDRRFEELAKAGRAGLVTFVMAGDPDLSTSLEILHALVRAGADVLEVGMPFTDPMADGPAIQAAGLRALKAGMTLR
KTLDMVAAFRAKDATTPIILMGYYNPIYIFGVEAFLNRAKEVGVDGLIVVDLPPEEDEELCLPARAAGLNFIRLATPTTD
AVRLPAVLANTSGFLYYVSITGITGAGVPDYSKVASAVAGIKAHTNLPVAVGFGVKTAESARTIACHADAVVVGSAIVDA
LRASLDGEGRATPACIGTVEKLVAELAEGVRSAAAAA

Sequences:

>Translated_277_residues
MPTRIDRRFEELAKAGRAGLVTFVMAGDPDLSTSLEILHALVRAGADVLEVGMPFTDPMADGPAIQAAGLRALKAGMTLR
KTLDMVAAFRAKDATTPIILMGYYNPIYIFGVEAFLNRAKEVGVDGLIVVDLPPEEDEELCLPARAAGLNFIRLATPTTD
AVRLPAVLANTSGFLYYVSITGITGAGVPDYSKVASAVAGIKAHTNLPVAVGFGVKTAESARTIACHADAVVVGSAIVDA
LRASLDGEGRATPACIGTVEKLVAELAEGVRSAAAAA
>Mature_276_residues
PTRIDRRFEELAKAGRAGLVTFVMAGDPDLSTSLEILHALVRAGADVLEVGMPFTDPMADGPAIQAAGLRALKAGMTLRK
TLDMVAAFRAKDATTPIILMGYYNPIYIFGVEAFLNRAKEVGVDGLIVVDLPPEEDEELCLPARAAGLNFIRLATPTTDA
VRLPAVLANTSGFLYYVSITGITGAGVPDYSKVASAVAGIKAHTNLPVAVGFGVKTAESARTIACHADAVVVGSAIVDAL
RASLDGEGRATPACIGTVEKLVAELAEGVRSAAAAA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=248, Percent_Identity=34.6774193548387, Blast_Score=136, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=41.0041841004184, Blast_Score=179, Evalue=6e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BEII9 (B2IF46)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833100.1
- GeneID:   6198983
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1988
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28677; Mature: 28546

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRIDRRFEELAKAGRAGLVTFVMAGDPDLSTSLEILHALVRAGADVLEVGMPFTDPMA
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
DGPAIQAAGLRALKAGMTLRKTLDMVAAFRAKDATTPIILMGYYNPIYIFGVEAFLNRAK
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
EVGVDGLIVVDLPPEEDEELCLPARAAGLNFIRLATPTTDAVRLPAVLANTSGFLYYVSI
HCCCCCEEEEECCCCCCCCEECCHHHCCCCEEEEECCCCCHHCCCHHEECCCCEEEEEEE
TGITGAGVPDYSKVASAVAGIKAHTNLPVAVGFGVKTAESARTIACHADAVVVGSAIVDA
ECCCCCCCCCHHHHHHHHHCCHHCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHH
LRASLDGEGRATPACIGTVEKLVAELAEGVRSAAAAA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTRIDRRFEELAKAGRAGLVTFVMAGDPDLSTSLEILHALVRAGADVLEVGMPFTDPMA
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
DGPAIQAAGLRALKAGMTLRKTLDMVAAFRAKDATTPIILMGYYNPIYIFGVEAFLNRAK
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
EVGVDGLIVVDLPPEEDEELCLPARAAGLNFIRLATPTTDAVRLPAVLANTSGFLYYVSI
HCCCCCEEEEECCCCCCCCEECCHHHCCCCEEEEECCCCCHHCCCHHEECCCCEEEEEEE
TGITGAGVPDYSKVASAVAGIKAHTNLPVAVGFGVKTAESARTIACHADAVVVGSAIVDA
ECCCCCCCCCHHHHHHHHHCCHHCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHH
LRASLDGEGRATPACIGTVEKLVAELAEGVRSAAAAA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA