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The map label for this gene is nfrA1 [H]

Identifier: 182678819

GI number: 182678819

Start: 2109996

End: 2110862

Strand: Direct

Name: nfrA1 [H]

Synonym: Bind_1852

Alternate gene names: 182678819

Gene position: 2109996-2110862 (Clockwise)

Preceding gene: 182678818

Following gene: 182678820

Centisome position: 50.6

GC content: 60.09

Gene sequence:

>867_bases
ATGACACATACGGAAACGATTGATCGGAACACGATTGAAGAGAAAAGCGACAGGACCCTTCATGACTTGATTTTCGCGCG
CTATCGTCAGCCCGTGGAGACGGGCACCGTCGCGCTCAATCCTGTCCTCGAAGCCATTCTCTCGCATCGATCCGTCAGGG
ATTATCTGCCGACCCCGCTGCCGCAAGGGGCGCTGGAGACGATCGTGGCGGCGGCGCAATCGGCCTCGACCTCGTCCAAT
CTGCAAGCCTGGAGCGTCGTTGCCGTCAAAGATCCCGACCGTCGGGCCCGCCTTGCGGCTCTTTCAGGACATCAACGGCA
GATCGTCCAGGCGCCTCTTTTCCTCGTCTGGCTCGCTGATCTGTCACGCCTTCGCGCCATCGCCAAAGGTCTCGACCGAA
CCTCGGAAGGTCTCGATTATATCGAGACCTTCCTGCTCGCCGTGATCGATGCGACACTGGCGGCACAGAATGCCGTCGTC
GCCCTGGAATCGCTCGGTCTTGGGTCCTGCTATATCGGCGGCATCCGCAATCAGCCGGAAGCGGTGGCGGCGGAACTACG
CCTGCCACCGGAGACCATCGCCATTTTCGGCCTGACGGTTGGTTATCCAGACCCGGACAAACCGGCCGAGATCAAACCCC
GTCTCTCCCAAAACATCGTGCTCCATCACGAGCTTTATGGCGAGGAGCATCCAGCGGCCCCGCTCGATCTCTATAATGCA
ACCCTGCGGATCTTTCAGGCGGAACAAAATCTGTCGCCGATCGACTGGACGGACCAGGCCACGAGCCGCATCAGCACGGC
TGACGCGCTCAAGGGGCGTCAATTGTTGACCGATGTCATCCGCGCGCTCGGCTTTGCCCTGAAATAA

Upstream 100 bases:

>100_bases
AATCTGGGCCTGCGCCGCCCGGCCAATCGCCATGTCCAAAGTGCCAAAGCGGCTGAATAAGACGGCTGAATGGAACTCTT
AAAAAAGCGGATGGTCATTC

Downstream 100 bases:

>100_bases
ATTCCCCGATCCGCCAAACTCGACCGCATGAGTTTGTGATCATTTTTCAGATTTGAAGAGTGCTGTCTGCGCATTCATGG
ATCGGCCAGCACTCAGGTCT

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTHTETIDRNTIEEKSDRTLHDLIFARYRQPVETGTVALNPVLEAILSHRSVRDYLPTPLPQGALETIVAAAQSASTSSN
LQAWSVVAVKDPDRRARLAALSGHQRQIVQAPLFLVWLADLSRLRAIAKGLDRTSEGLDYIETFLLAVIDATLAAQNAVV
ALESLGLGSCYIGGIRNQPEAVAAELRLPPETIAIFGLTVGYPDPDKPAEIKPRLSQNIVLHHELYGEEHPAAPLDLYNA
TLRIFQAEQNLSPIDWTDQATSRISTADALKGRQLLTDVIRALGFALK

Sequences:

>Translated_288_residues
MTHTETIDRNTIEEKSDRTLHDLIFARYRQPVETGTVALNPVLEAILSHRSVRDYLPTPLPQGALETIVAAAQSASTSSN
LQAWSVVAVKDPDRRARLAALSGHQRQIVQAPLFLVWLADLSRLRAIAKGLDRTSEGLDYIETFLLAVIDATLAAQNAVV
ALESLGLGSCYIGGIRNQPEAVAAELRLPPETIAIFGLTVGYPDPDKPAEIKPRLSQNIVLHHELYGEEHPAAPLDLYNA
TLRIFQAEQNLSPIDWTDQATSRISTADALKGRQLLTDVIRALGFALK
>Mature_287_residues
THTETIDRNTIEEKSDRTLHDLIFARYRQPVETGTVALNPVLEAILSHRSVRDYLPTPLPQGALETIVAAAQSASTSSNL
QAWSVVAVKDPDRRARLAALSGHQRQIVQAPLFLVWLADLSRLRAIAKGLDRTSEGLDYIETFLLAVIDATLAAQNAVVA
LESLGLGSCYIGGIRNQPEAVAAELRLPPETIAIFGLTVGYPDPDKPAEIKPRLSQNIVLHHELYGEEHPAAPLDLYNAT
LRIFQAEQNLSPIDWTDQATSRISTADALKGRQLLTDVIRALGFALK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=188, Percent_Identity=39.3617021276596, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 31506; Mature: 31375

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTETIDRNTIEEKSDRTLHDLIFARYRQPVETGTVALNPVLEAILSHRSVRDYLPTPL
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
PQGALETIVAAAQSASTSSNLQAWSVVAVKDPDRRARLAALSGHQRQIVQAPLFLVWLAD
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LSRLRAIAKGLDRTSEGLDYIETFLLAVIDATLAAQNAVVALESLGLGSCYIGGIRNQPE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
AVAAELRLPPETIAIFGLTVGYPDPDKPAEIKPRLSQNIVLHHELYGEEHPAAPLDLYNA
HHHHHHCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHH
TLRIFQAEQNLSPIDWTDQATSRISTADALKGRQLLTDVIRALGFALK
HHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THTETIDRNTIEEKSDRTLHDLIFARYRQPVETGTVALNPVLEAILSHRSVRDYLPTPL
CCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
PQGALETIVAAAQSASTSSNLQAWSVVAVKDPDRRARLAALSGHQRQIVQAPLFLVWLAD
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LSRLRAIAKGLDRTSEGLDYIETFLLAVIDATLAAQNAVVALESLGLGSCYIGGIRNQPE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
AVAAELRLPPETIAIFGLTVGYPDPDKPAEIKPRLSQNIVLHHELYGEEHPAAPLDLYNA
HHHHHHCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHH
TLRIFQAEQNLSPIDWTDQATSRISTADALKGRQLLTDVIRALGFALK
HHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]