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The map label for this gene is nemA [H]

Identifier: 182678803

GI number: 182678803

Start: 2084932

End: 2086059

Strand: Direct

Name: nemA [H]

Synonym: Bind_1834

Alternate gene names: 182678803

Gene position: 2084932-2086059 (Clockwise)

Preceding gene: 182678801

Following gene: 182678804

Centisome position: 50.0

GC content: 63.21

Gene sequence:

>1128_bases
ATGTCGAACCTAGCAAAGCTGTTCACCCCAATCAAAGTCGGTGCTTACACCCTGTCGCACCGGGTCGTGCTCGCGCCGAT
GACGAGGCTGCGGACTATTCAGCCGGGCGATATCCCGAGCCCGATGATGGCCGATTTCTACGGTCAGCGTGCCTCGCAAG
GTGGTCTCGAGATCATCGAAGGCGTCAGCATTTCCATCCCCGCCCGCTCCTATCTGGGCGCGGCGAGCTTCTATCACGAC
GGTCAGGTCGAGGGCTGGAAGGCGATTGCCGACGCGGTTCATGCCAAGGGTGGCCGCGTCTTCATGCAGCTCATTCATGG
CGGTCGGCAGAGTCATATCGATATGACTGGCGGCGTCGCTCCGGTCGCGCCTTCGGTCGTTCCCTTTGACGGCGTGGCCT
TGACCAAGGACGGCTGGGTGCCGGTCTCTCCGCACCGCGCGCTGGAGATCGAGGAGATCCCGGGCATCGTCGAGGAATTC
CGCGTCGCGGCCCAACGCGCGCTCGATGCCGGCTTCGACGGGGTCGAGTTGCACGGCGCCAACGGCTATCTGGTCGACCA
GTTCATCCAGGACGGCACCAACAAGCGCACTGATGCCTATGGTGGGCCAATTGAGAATCGCGTGCGTTTCCTGAAGGAAG
CGGTCGAAGCGCTTATCTCCGTCTGGGGTGCCGATCGGGTCGGCGTCCGCATCTCGCCATCGGGCGAATGGGGCGGCATC
TCGGACAGCGATCCGGAAGCGACCATCTCTTATGTGGCGCAGTTCCTCGATCAATATGGTCTCGCCTACCTGCACGTCAT
CGAACCGCGCATCAAGGGTGACTCGACCCTCCACGAGGGCCATCCCCCGGTCGCGGTCAAGTTTCTCCGCCCGCATTTCT
CCGGCCCGATCATCGCCGCCGGCGGCTTCGATCGTGAAAGTGCGATTCACATCGTCGAAGCCGGTGACGCTGATCTTGTC
GCCTTCGGCCGTCACTTCACCTCGAACCCGGACCTGCCCTATCGCCTGAAGCACAATGTCCCGCTGACGCCCTATGTGCG
TGATGCCTTCTGGGGCGGCAACGAGAAGGGCTACACGGACTTTCCCGCCATCGAACCCGCCACCGCCGGACTCGCCGCGA
CGGCCTGA

Upstream 100 bases:

>100_bases
GAATGAGCAGGTTAGGCCACAGCGCAGCATGAGCTGCCAGCAACCAGGGTCCCCTGGGGCGACGTCACGAGAGCCCCAGC
TGGAGAGTCGGAGAGCTGTT

Downstream 100 bases:

>100_bases
GCCCAAGTGCCAAGCCTCGCCCATCCAAACGGAGATTCACATGTCCCGCACCATCAAATTCTCCAAGGCCGGCGGTCCGG
AAGTCCTCGAGTTCATCGAG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSNLAKLFTPIKVGAYTLSHRVVLAPMTRLRTIQPGDIPSPMMADFYGQRASQGGLEIIEGVSISIPARSYLGAASFYHD
GQVEGWKAIADAVHAKGGRVFMQLIHGGRQSHIDMTGGVAPVAPSVVPFDGVALTKDGWVPVSPHRALEIEEIPGIVEEF
RVAAQRALDAGFDGVELHGANGYLVDQFIQDGTNKRTDAYGGPIENRVRFLKEAVEALISVWGADRVGVRISPSGEWGGI
SDSDPEATISYVAQFLDQYGLAYLHVIEPRIKGDSTLHEGHPPVAVKFLRPHFSGPIIAAGGFDRESAIHIVEAGDADLV
AFGRHFTSNPDLPYRLKHNVPLTPYVRDAFWGGNEKGYTDFPAIEPATAGLAATA

Sequences:

>Translated_375_residues
MSNLAKLFTPIKVGAYTLSHRVVLAPMTRLRTIQPGDIPSPMMADFYGQRASQGGLEIIEGVSISIPARSYLGAASFYHD
GQVEGWKAIADAVHAKGGRVFMQLIHGGRQSHIDMTGGVAPVAPSVVPFDGVALTKDGWVPVSPHRALEIEEIPGIVEEF
RVAAQRALDAGFDGVELHGANGYLVDQFIQDGTNKRTDAYGGPIENRVRFLKEAVEALISVWGADRVGVRISPSGEWGGI
SDSDPEATISYVAQFLDQYGLAYLHVIEPRIKGDSTLHEGHPPVAVKFLRPHFSGPIIAAGGFDRESAIHIVEAGDADLV
AFGRHFTSNPDLPYRLKHNVPLTPYVRDAFWGGNEKGYTDFPAIEPATAGLAATA
>Mature_374_residues
SNLAKLFTPIKVGAYTLSHRVVLAPMTRLRTIQPGDIPSPMMADFYGQRASQGGLEIIEGVSISIPARSYLGAASFYHDG
QVEGWKAIADAVHAKGGRVFMQLIHGGRQSHIDMTGGVAPVAPSVVPFDGVALTKDGWVPVSPHRALEIEEIPGIVEEFR
VAAQRALDAGFDGVELHGANGYLVDQFIQDGTNKRTDAYGGPIENRVRFLKEAVEALISVWGADRVGVRISPSGEWGGIS
DSDPEATISYVAQFLDQYGLAYLHVIEPRIKGDSTLHEGHPPVAVKFLRPHFSGPIIAAGGFDRESAIHIVEAGDADLVA
FGRHFTSNPDLPYRLKHNVPLTPYVRDAFWGGNEKGYTDFPAIEPATAGLAATA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=45.0819672131148, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789463, Length=335, Percent_Identity=29.5522388059701, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=341, Percent_Identity=29.0322580645161, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=342, Percent_Identity=29.2397660818713, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=293, Percent_Identity=28.6689419795222, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=359, Percent_Identity=26.7409470752089, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=297, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=345, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=37.9947229551451, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=379, Percent_Identity=35.3562005277045, Blast_Score=203, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40340; Mature: 40209

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLAKLFTPIKVGAYTLSHRVVLAPMTRLRTIQPGDIPSPMMADFYGQRASQGGLEIIE
CCCHHHHHHHHHHCEEEECCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECC
GVSISIPARSYLGAASFYHDGQVEGWKAIADAVHAKGGRVFMQLIHGGRQSHIDMTGGVA
CCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCC
PVAPSVVPFDGVALTKDGWVPVSPHRALEIEEIPGIVEEFRVAAQRALDAGFDGVELHGA
CCCCCCCCCCCEEECCCCCEECCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEECC
NGYLVDQFIQDGTNKRTDAYGGPIENRVRFLKEAVEALISVWGADRVGVRISPSGEWGGI
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
SDSDPEATISYVAQFLDQYGLAYLHVIEPRIKGDSTLHEGHPPVAVKFLRPHFSGPIIAA
CCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
GGFDRESAIHIVEAGDADLVAFGRHFTSNPDLPYRLKHNVPLTPYVRDAFWGGNEKGYTD
CCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCC
FPAIEPATAGLAATA
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNLAKLFTPIKVGAYTLSHRVVLAPMTRLRTIQPGDIPSPMMADFYGQRASQGGLEIIE
CCHHHHHHHHHHCEEEECCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECC
GVSISIPARSYLGAASFYHDGQVEGWKAIADAVHAKGGRVFMQLIHGGRQSHIDMTGGVA
CCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCC
PVAPSVVPFDGVALTKDGWVPVSPHRALEIEEIPGIVEEFRVAAQRALDAGFDGVELHGA
CCCCCCCCCCCEEECCCCCEECCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEECC
NGYLVDQFIQDGTNKRTDAYGGPIENRVRFLKEAVEALISVWGADRVGVRISPSGEWGGI
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
SDSDPEATISYVAQFLDQYGLAYLHVIEPRIKGDSTLHEGHPPVAVKFLRPHFSGPIIAA
CCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
GGFDRESAIHIVEAGDADLVAFGRHFTSNPDLPYRLKHNVPLTPYVRDAFWGGNEKGYTD
CCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCC
FPAIEPATAGLAATA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]