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The map label for this gene is alkB [H]

Identifier: 182678752

GI number: 182678752

Start: 2020116

End: 2020814

Strand: Reverse

Name: alkB [H]

Synonym: Bind_1781

Alternate gene names: 182678752

Gene position: 2020814-2020116 (Counterclockwise)

Preceding gene: 182678753

Following gene: 182678751

Centisome position: 48.46

GC content: 57.22

Gene sequence:

>699_bases
ATGATCGAATATTGGCCTTCCAAAAGGACATCTGATTCAATCGTGCGGCAAAACGAGTCTTTGCTCCTGCCTTTTGAAAC
ACAACGGATCATCGAGCCCTTCGCGGACGGGGCCGTCCTCTTGCGCGCCGAAGTCCTTGACAAAGCAGAGGGTCTTTTGG
AAGAGGTGAGGGCTATAGCCCATGCGGCTCCTTTTCGGCGCATGGTGACGCCGGGCGGTTTCACCATGTCGGTCGCCATG
ACCAATTGTGGCGATACGGGGTGGATCACCGATCGGCGGGGCTATCGCTATGGGGCCATAGACCCCGAAAGCGGTCACCA
ATGGCCAGCCATGCCCGCTTTGTTCAGAGCCGTGGCCGTGACAGCGGCGGAGAGAGCTGGGTACGTCGGTTTCGAGCCGG
ATGCCTGCCTCATCAATCGTTATGAACCCGGCAGCCGCATGTCATTGCATCAAGATAAGAACGAGAGAGATTTTGCGCAG
CCGATCGTTTCCGTCTCGCTTGGGCTGTTGGCGACTTTCCAATTTGGAGGCGCCACGCGGAAGGACCCAGTCCGTAAAAT
TTTTCTCGACCATGCGGATGTCGTGGTTTGGGGTGGCGCCTCGCGACTGAACCACCACGGCGTGTTGACACTGAAAAACG
GGTGGCATCCGAGCACAGGTCGGGTGCGGTATAATCTCACATTCCGGAAAGCGCTCTAA

Upstream 100 bases:

>100_bases
AATGCGCTTGAAACGCTTCTGCTTCTGTCACGCGCTGATTGCAAAATCCCCAACGATGAGTGCCAAGCGTAACGCTTGGC
CTGTGCTGTAGCGATCCTTG

Downstream 100 bases:

>100_bases
ATTCACAGAATTGAGTATGTCATAGAATCTTTAATTCTTGACGTGCTATATATTAATAGATATAGAATACGGGCGAAACG
AGAATGCTGGCCTTGATGAG

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIEYWPSKRTSDSIVRQNESLLLPFETQRIIEPFADGAVLLRAEVLDKAEGLLEEVRAIAHAAPFRRMVTPGGFTMSVAM
TNCGDTGWITDRRGYRYGAIDPESGHQWPAMPALFRAVAVTAAERAGYVGFEPDACLINRYEPGSRMSLHQDKNERDFAQ
PIVSVSLGLLATFQFGGATRKDPVRKIFLDHADVVVWGGASRLNHHGVLTLKNGWHPSTGRVRYNLTFRKAL

Sequences:

>Translated_232_residues
MIEYWPSKRTSDSIVRQNESLLLPFETQRIIEPFADGAVLLRAEVLDKAEGLLEEVRAIAHAAPFRRMVTPGGFTMSVAM
TNCGDTGWITDRRGYRYGAIDPESGHQWPAMPALFRAVAVTAAERAGYVGFEPDACLINRYEPGSRMSLHQDKNERDFAQ
PIVSVSLGLLATFQFGGATRKDPVRKIFLDHADVVVWGGASRLNHHGVLTLKNGWHPSTGRVRYNLTFRKAL
>Mature_232_residues
MIEYWPSKRTSDSIVRQNESLLLPFETQRIIEPFADGAVLLRAEVLDKAEGLLEEVRAIAHAAPFRRMVTPGGFTMSVAM
TNCGDTGWITDRRGYRYGAIDPESGHQWPAMPALFRAVAVTAAERAGYVGFEPDACLINRYEPGSRMSLHQDKNERDFAQ
PIVSVSLGLLATFQFGGATRKDPVRKIFLDHADVVVWGGASRLNHHGVLTLKNGWHPSTGRVRYNLTFRKAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=200, Percent_Identity=59, Blast_Score=253, Evalue=8e-69,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=33.6734693877551, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25854; Mature: 25854

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEYWPSKRTSDSIVRQNESLLLPFETQRIIEPFADGAVLLRAEVLDKAEGLLEEVRAIA
CCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
HAAPFRRMVTPGGFTMSVAMTNCGDTGWITDRRGYRYGAIDPESGHQWPAMPALFRAVAV
HHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEECCCCCCCCCCCCCHHHHHHHHHH
TAAERAGYVGFEPDACLINRYEPGSRMSLHQDKNERDFAQPIVSVSLGLLATFQFGGATR
HHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCC
KDPVRKIFLDHADVVVWGGASRLNHHGVLTLKNGWHPSTGRVRYNLTFRKAL
CCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MIEYWPSKRTSDSIVRQNESLLLPFETQRIIEPFADGAVLLRAEVLDKAEGLLEEVRAIA
CCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
HAAPFRRMVTPGGFTMSVAMTNCGDTGWITDRRGYRYGAIDPESGHQWPAMPALFRAVAV
HHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEECCCCCCCCCCCCCHHHHHHHHHH
TAAERAGYVGFEPDACLINRYEPGSRMSLHQDKNERDFAQPIVSVSLGLLATFQFGGATR
HHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCC
KDPVRKIFLDHADVVVWGGASRLNHHGVLTLKNGWHPSTGRVRYNLTFRKAL
CCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]