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The map label for this gene is rna [H]

Identifier: 182678670

GI number: 182678670

Start: 1919396

End: 1920409

Strand: Reverse

Name: rna [H]

Synonym: Bind_1696

Alternate gene names: 182678670

Gene position: 1920409-1919396 (Counterclockwise)

Preceding gene: 182678673

Following gene: 182678669

Centisome position: 46.05

GC content: 55.62

Gene sequence:

>1014_bases
ATGAAAAAGATCTTGATCGGCGCTTTGTTGCTCGCCACACCGCTTTGTATCCGTCAAGCCATGGCGGATGTTCCCCTGTC
CGGTTATTTCATCGCGGGGCAGGCCTGTCCGGCTTTGCAATCGATCAAGAAAGACACAAATCCCGGTTCGATCACGACGA
AAGAGCATTATGCCTATCCCCTCACGGCAAAGAATAAACCAGCGGCGTCGCATTATTTGATCGAAGTCGAGGGCGCGGAC
CCTCTGCGGCGCTGGGTCGCCGTGACCTGTGGGGAATATGTCATTCCGGTTGATGGCTCGGTTTCCCCTGGCCCCGTGGT
TTTTCAACCAGACACCGGAGGCGGGCGTCCGACGCAAAAGGCGGAATATGTTCTCGCCATAAGCTGGCAGCCGGCTTTCT
GCGAAGGCAAGCCTGACAAACCTGAGTGCAAGACGCAGACAGCCAATCGTTTCGATACCGATCATTTCACTTTGCATGGC
CTTTGGCCGCAGCCCCTGACGAATATATTCTGTCACGTCTCGCCGGAACTCGTCAGTGCGGATAAAAATGGTGATTGGGA
CAAACTTCCCGAGCCGAATCTCGATGAGACGACACGCAAGAGCCTGGAAGAAGTCATGCCGGGCATGATGTCTCATCTGG
AACGCCATGAATGGATCAAACACGGCACCTGCTTCAACGGGGAGACCGCCGACACATATTTTTCACGCGCTCTCACGCTC
GCGAACCAACTCAATACATCCAAGGTGCGCGGGCTGTTTGCCAGTCACATCGGGTCCGAAATTACGATTCAGCAGATCAA
GGACGCTTTCGACGATAGTTTTGGCGATGGCGCGGGGGATCGCGTGCGTGTTGCCTGTAAGCGAGACGGGACGCGAAACC
TGATCGGCGAGCTCACGATCGGCCTCGTCGGCGAAATCGACGAAAATGCCTCGCTCGCCAATTTGATCGCTGCCTCGTCA
CCAACCGATCCCGGTTGCCCTCGTGGCATTGTCGATCCTGTCGGTTTTCAATGA

Upstream 100 bases:

>100_bases
TCTGGTAGCCCCCTTATTGTCTTCTCAATGATACATTCCGGTTCTACGCTATCATCAATAATCAGCCTTCAATATCGAGA
TCGCGATACAAGGGGGGACT

Downstream 100 bases:

>100_bases
AGACGGCGCGCTTGCTTAGGGCAATATGAGACTTCGAAAGTGTTGCACCGTTCAAGCCTGCGACCAATGGCTTGGGACCT
TCGATTTTGCACGACGATCG

Product: ribonuclease T2

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKKILIGALLLATPLCIRQAMADVPLSGYFIAGQACPALQSIKKDTNPGSITTKEHYAYPLTAKNKPAASHYLIEVEGAD
PLRRWVAVTCGEYVIPVDGSVSPGPVVFQPDTGGGRPTQKAEYVLAISWQPAFCEGKPDKPECKTQTANRFDTDHFTLHG
LWPQPLTNIFCHVSPELVSADKNGDWDKLPEPNLDETTRKSLEEVMPGMMSHLERHEWIKHGTCFNGETADTYFSRALTL
ANQLNTSKVRGLFASHIGSEITIQQIKDAFDDSFGDGAGDRVRVACKRDGTRNLIGELTIGLVGEIDENASLANLIAASS
PTDPGCPRGIVDPVGFQ

Sequences:

>Translated_337_residues
MKKILIGALLLATPLCIRQAMADVPLSGYFIAGQACPALQSIKKDTNPGSITTKEHYAYPLTAKNKPAASHYLIEVEGAD
PLRRWVAVTCGEYVIPVDGSVSPGPVVFQPDTGGGRPTQKAEYVLAISWQPAFCEGKPDKPECKTQTANRFDTDHFTLHG
LWPQPLTNIFCHVSPELVSADKNGDWDKLPEPNLDETTRKSLEEVMPGMMSHLERHEWIKHGTCFNGETADTYFSRALTL
ANQLNTSKVRGLFASHIGSEITIQQIKDAFDDSFGDGAGDRVRVACKRDGTRNLIGELTIGLVGEIDENASLANLIAASS
PTDPGCPRGIVDPVGFQ
>Mature_337_residues
MKKILIGALLLATPLCIRQAMADVPLSGYFIAGQACPALQSIKKDTNPGSITTKEHYAYPLTAKNKPAASHYLIEVEGAD
PLRRWVAVTCGEYVIPVDGSVSPGPVVFQPDTGGGRPTQKAEYVLAISWQPAFCEGKPDKPECKTQTANRFDTDHFTLHG
LWPQPLTNIFCHVSPELVSADKNGDWDKLPEPNLDETTRKSLEEVMPGMMSHLERHEWIKHGTCFNGETADTYFSRALTL
ANQLNTSKVRGLFASHIGSEITIQQIKDAFDDSFGDGAGDRVRVACKRDGTRNLIGELTIGLVGEIDENASLANLIAASS
PTDPGCPRGIVDPVGFQ

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=246, Percent_Identity=27.2357723577236, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 36602; Mature: 36602

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIGALLLATPLCIRQAMADVPLSGYFIAGQACPALQSIKKDTNPGSITTKEHYAYP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCEECCCCEEEE
LTAKNKPAASHYLIEVEGADPLRRWVAVTCGEYVIPVDGSVSPGPVVFQPDTGGGRPTQK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCCC
AEYVLAISWQPAFCEGKPDKPECKTQTANRFDTDHFTLHGLWPQPLTNIFCHVSPELVSA
CCEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCEEEECCCCCCHHHHEEECCHHHHCC
DKNGDWDKLPEPNLDETTRKSLEEVMPGMMSHLERHEWIKHGTCFNGETADTYFSRALTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ANQLNTSKVRGLFASHIGSEITIQQIKDAFDDSFGDGAGDRVRVACKRDGTRNLIGELTI
HHHCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
GLVGEIDENASLANLIAASSPTDPGCPRGIVDPVGFQ
HEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKILIGALLLATPLCIRQAMADVPLSGYFIAGQACPALQSIKKDTNPGSITTKEHYAYP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCEECCCCEEEE
LTAKNKPAASHYLIEVEGADPLRRWVAVTCGEYVIPVDGSVSPGPVVFQPDTGGGRPTQK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCCC
AEYVLAISWQPAFCEGKPDKPECKTQTANRFDTDHFTLHGLWPQPLTNIFCHVSPELVSA
CCEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCEEEECCCCCCHHHHEEECCHHHHCC
DKNGDWDKLPEPNLDETTRKSLEEVMPGMMSHLERHEWIKHGTCFNGETADTYFSRALTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ANQLNTSKVRGLFASHIGSEITIQQIKDAFDDSFGDGAGDRVRVACKRDGTRNLIGELTI
HHHCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
GLVGEIDENASLANLIAASSPTDPGCPRGIVDPVGFQ
HEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]