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The map label for this gene is sbp [H]

Identifier: 182678664

GI number: 182678664

Start: 1909415

End: 1910446

Strand: Reverse

Name: sbp [H]

Synonym: Bind_1690

Alternate gene names: 182678664

Gene position: 1910446-1909415 (Counterclockwise)

Preceding gene: 182678665

Following gene: 182678663

Centisome position: 45.81

GC content: 57.36

Gene sequence:

>1032_bases
ATGCGGATTTCCATCATCAATATTGGCGCGGTGGCGGGGGTCGCCATAGCTCTTGCTCTGCTCATCACCAAGAACAGCGA
AGGGGGCGCCGCCCATTATTTATTGAACGTTTCCTATGATCCAACGCGCGAACTTTATCGCGATATCAATCCGCGTTTTG
TCGAGAAATATGAGGCCGAGACCGGGTCGAGCGTTGGGATCAAGCAATCGCATGGCGGCTCGTCCTATCAGGCGCGTCTG
GTCGCCACGGGTGAACTCCAGGCCGATGTCGTCACATTGGGCCTGCCGTCCGATGTCGAAGGGCTGCACAAGCGGGGGCT
GCTCGCTGATGGATGGGACCAGCGTCTGCCACATGCGTCCCAGCCCTATGTTTCGACAATCGTCTTCGTGGTGCGCAAGG
GAAATCCCCATGCCATTCACGACTGGCCGGACCTGTTGAAGTCTGGGATCGATGTCATTGTTCCCGATCCTAAAACCTCC
GGCAATGGAAAAGCGGCGGCGCTCGCCGCCTGGGGCGCGGTCGTCACACGCGGTGGCAGCGAGAAGGAAGCGCGCGACTT
TCTGAAGGCGCTTTACGATCACGCCCCTTTCCTCGATCCGGCGGCGCGCTCGGCCGGGGTCGCCTTTGCGATCGAAAAGA
TCGGTGATGTTCATCTCGCCTGGGAAAATGAAGGCCTGCGCGAAATCGAACAATCGCAGGGCGCTGTCGAGATTGTCTAT
CCGCCGACCAGCTTGCGTATCGAACCGACTGTTGCTTGGGTTGATAGCAATGTCAGCAAACATGGCACCGAGAAAATAGC
CCGCGCCTATCTGGAATTCCTGTTTAGTGATGAGGCTCAGGAAATCATCGCGCGCAACGGCTATCGGCCGTCCAACCCCG
ACATATTGGCGAAACACGCGGATCGTCTGCCTGACATCAACCTTTTTCCGATTACTGCCATCGCGCGGGACTGGGCCGAT
GCTCAGCAGAAGTTCTTCGCGGAAAACGGCATTATCGACACGATCTATGTTCCCAAGCCTCGCACAAATTGA

Upstream 100 bases:

>100_bases
ATGAAATTAAAACATCTCCATGACACTCTTGACACGCTCATAAATACATTCTTTCTATATATTTAATCAATTAAGCTAGG
GCGTGATGTCGAGGGCAGCG

Downstream 100 bases:

>100_bases
TGCGTTATCCCGGCGTCGCCAATGTTTTCGTCTCTCGCGTGTAGGCGCGGAAGCTACGGCAACGACTTTGAATATTGCCT
TTGAGAATTTTCCGCGCGGC

Product: sulfate ABC transporter periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRISIINIGAVAGVAIALALLITKNSEGGAAHYLLNVSYDPTRELYRDINPRFVEKYEAETGSSVGIKQSHGGSSYQARL
VATGELQADVVTLGLPSDVEGLHKRGLLADGWDQRLPHASQPYVSTIVFVVRKGNPHAIHDWPDLLKSGIDVIVPDPKTS
GNGKAAALAAWGAVVTRGGSEKEARDFLKALYDHAPFLDPAARSAGVAFAIEKIGDVHLAWENEGLREIEQSQGAVEIVY
PPTSLRIEPTVAWVDSNVSKHGTEKIARAYLEFLFSDEAQEIIARNGYRPSNPDILAKHADRLPDINLFPITAIARDWAD
AQQKFFAENGIIDTIYVPKPRTN

Sequences:

>Translated_343_residues
MRISIINIGAVAGVAIALALLITKNSEGGAAHYLLNVSYDPTRELYRDINPRFVEKYEAETGSSVGIKQSHGGSSYQARL
VATGELQADVVTLGLPSDVEGLHKRGLLADGWDQRLPHASQPYVSTIVFVVRKGNPHAIHDWPDLLKSGIDVIVPDPKTS
GNGKAAALAAWGAVVTRGGSEKEARDFLKALYDHAPFLDPAARSAGVAFAIEKIGDVHLAWENEGLREIEQSQGAVEIVY
PPTSLRIEPTVAWVDSNVSKHGTEKIARAYLEFLFSDEAQEIIARNGYRPSNPDILAKHADRLPDINLFPITAIARDWAD
AQQKFFAENGIIDTIYVPKPRTN
>Mature_343_residues
MRISIINIGAVAGVAIALALLITKNSEGGAAHYLLNVSYDPTRELYRDINPRFVEKYEAETGSSVGIKQSHGGSSYQARL
VATGELQADVVTLGLPSDVEGLHKRGLLADGWDQRLPHASQPYVSTIVFVVRKGNPHAIHDWPDLLKSGIDVIVPDPKTS
GNGKAAALAAWGAVVTRGGSEKEARDFLKALYDHAPFLDPAARSAGVAFAIEKIGDVHLAWENEGLREIEQSQGAVEIVY
PPTSLRIEPTVAWVDSNVSKHGTEKIARAYLEFLFSDEAQEIIARNGYRPSNPDILAKHADRLPDINLFPITAIARDWAD
AQQKFFAENGIIDTIYVPKPRTN

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=322, Percent_Identity=45.3416149068323, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=41.2903225806452, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37346; Mature: 37346

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISIINIGAVAGVAIALALLITKNSEGGAAHYLLNVSYDPTRELYRDINPRFVEKYEAE
CEEEEEECHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHC
TGSSVGIKQSHGGSSYQARLVATGELQADVVTLGLPSDVEGLHKRGLLADGWDQRLPHAS
CCCCCCEECCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCCCHHHHCCCCC
QPYVSTIVFVVRKGNPHAIHDWPDLLKSGIDVIVPDPKTSGNGKAAALAAWGAVVTRGGS
CCHHEEEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCEEHHHHHHHHHCCCCC
EKEARDFLKALYDHAPFLDPAARSAGVAFAIEKIGDVHLAWENEGLREIEQSQGAVEIVY
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHCCCCEEEEE
PPTSLRIEPTVAWVDSNVSKHGTEKIARAYLEFLFSDEAQEIIARNGYRPSNPDILAKHA
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHH
DRLPDINLFPITAIARDWADAQQKFFAENGIIDTIYVPKPRTN
HCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MRISIINIGAVAGVAIALALLITKNSEGGAAHYLLNVSYDPTRELYRDINPRFVEKYEAE
CEEEEEECHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHC
TGSSVGIKQSHGGSSYQARLVATGELQADVVTLGLPSDVEGLHKRGLLADGWDQRLPHAS
CCCCCCEECCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCCCHHHHCCCCC
QPYVSTIVFVVRKGNPHAIHDWPDLLKSGIDVIVPDPKTSGNGKAAALAAWGAVVTRGGS
CCHHEEEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCEEHHHHHHHHHCCCCC
EKEARDFLKALYDHAPFLDPAARSAGVAFAIEKIGDVHLAWENEGLREIEQSQGAVEIVY
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHCCCCEEEEE
PPTSLRIEPTVAWVDSNVSKHGTEKIARAYLEFLFSDEAQEIIARNGYRPSNPDILAKHA
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHH
DRLPDINLFPITAIARDWADAQQKFFAENGIIDTIYVPKPRTN
HCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]