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The map label for this gene is pitA [H]

Identifier: 182678611

GI number: 182678611

Start: 1849848

End: 1851467

Strand: Direct

Name: pitA [H]

Synonym: Bind_1637

Alternate gene names: 182678611

Gene position: 1849848-1851467 (Clockwise)

Preceding gene: 182678610

Following gene: 182678614

Centisome position: 44.36

GC content: 57.16

Gene sequence:

>1620_bases
ATGGTCGATGCAACAGCACCAACATACTACCAGGAAAATGAAACGCCTGAACGGGTGAAGCTTGATGAACCCATGGGCAT
CCGTACACTATTTGGTTTACTTACTGCAATTGCTTTAGGTCTATTTTTTACCGCTTACAGTATATTTGTTGATGTTTCCG
ATGCTGGCGCCAAGATCACGAGTATCTTGCCATTTCTGTTGCTCGGTGTCGCGCTGTTTATCGCGCTGGCTTTTGAATTC
GTCAACGGTTTCCACGATACGGCCAATGCCGTGGCGACTGTCATTTACACGCGGTCGCTCCAGCCGCAGGTCGCGGTTGT
CTGGTCCGGCTTCTTCAACTTTCTCGGGGTCCTCACCTCAACCGGAGCGGTCGCTTTCGGCATTGTTTCATTGCTCCCGG
TTGAACTGATCCTGCAAGTCGGCTCCGAAGCCGGCTTTGCCATGGTGTTCGCCATGCTGATCGCGGCGATCATCTGGAAT
CTCGGGACCTGGTGGTTCGGCCTGCCGGCCTCGAGCTCGCATACGCTCATTGGTTCGATCATTGGCGTCGGCGTTACCAA
TGCGCTCATGCGCGGCCGCGATGGGACAGCGGGTGTTGACTGGGGCAAGGCCACGGAAATCGGCTATGCTCTCTTGCTTT
CACCGCTGGTTGGCTTCATCTGCTCCGCTTTGCTGCTCCTCGTGATGCGGGCCTTGATCCGCAATCCCGCGCTTTATGGT
GAGCCGAAAGGCAATAGCAAGCCGCCGCTTTGGATTCGTGGTCTGCTGGTCTTGACCTGCACGGGCGTTTCCTTTGCGCA
TGGTTCGAACGACGGCCAAAAAGGCATGGGCTTAATCATGCTGATCCTCATCGGCACCGTGCCGACCGCCTATGCCTTGA
ACAGGACCTTGCCGGCGAGCCACGTCGAGGCTTTCGTGCAAGCTTCGGAAGCGGCCTCCACGGTGGTCGAGGCCAAGGCT
TCGGGTCATGCAATCCTGGGCGATCCGCGTCCAGCCGTGACGGCCTATCTCACGCAGCATGAAATCAGCGAGGGGACTTT
TCCGTCGCTTGCCGTGCTGATCCGCGATATTGCCCATCAGGTCACGAGCTATGGTGCCATTGCCAAGATTCCTCTGCAAG
AAGCTGGCAATACGCGTAACGATATGTATCTCGCCTCCGAGGCCTTGCGTTTCCTTGCCAAGGACAAGGCCAGCGAGCTT
TCTGCTGAGGATCTCACCATCCTCGGCAACTACAAGAAACAGCTCGACTCGGCGACGAAATTCATTCCTCCGTGGGTTAA
GGTCTCCGTGGCGATCGCCCTTGGCCTCGGCACGATGGTCGGCTGGAAACGCATCGTCGTCACCGTCGGCGAGAAAATCG
GCAAGACGCATTTGACCTATGGTCAAGGAGCCGCCGCTGAACTTGTCGCCATGGGGACGATTTTCGTGGCGGATACTTAT
GGCTTGCCGGTCTCGACCACGCATGTGCTCTCGTCTGGTGTCGCCGGCACGATGGCAGCCAACGGATCCGGCGTTCAACT
CGGAACTTTGCGCAATATCGCACTCGCCTGGGTTTTGACGCTGCCCTGCGCGATGTGCCTCTCGGGCTTCCTGTTCTGGT
CGTTCCGGCAGCTTTTCTGA

Upstream 100 bases:

>100_bases
GGCAGCTTGAGTAAATAAGCAAGCTATGTCGTAAAATTCCAAGCTGTCATGGTAATGTCGCTTCGCATCATTAAGCCTAT
CCGTAACAATGGGGGCGGTT

Downstream 100 bases:

>100_bases
TCTCGCCTATCTTTTCCAAGAAGAGCTTCAAGCCTCGTCGATGATCTTTCATCGGCGAGGCTTTTTGTTTGTGTGCACAA
ACAATCATAATCTTGGTGTG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MVDATAPTYYQENETPERVKLDEPMGIRTLFGLLTAIALGLFFTAYSIFVDVSDAGAKITSILPFLLLGVALFIALAFEF
VNGFHDTANAVATVIYTRSLQPQVAVVWSGFFNFLGVLTSTGAVAFGIVSLLPVELILQVGSEAGFAMVFAMLIAAIIWN
LGTWWFGLPASSSHTLIGSIIGVGVTNALMRGRDGTAGVDWGKATEIGYALLLSPLVGFICSALLLLVMRALIRNPALYG
EPKGNSKPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPASHVEAFVQASEAASTVVEAKA
SGHAILGDPRPAVTAYLTQHEISEGTFPSLAVLIRDIAHQVTSYGAIAKIPLQEAGNTRNDMYLASEALRFLAKDKASEL
SAEDLTILGNYKKQLDSATKFIPPWVKVSVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAELVAMGTIFVADTY
GLPVSTTHVLSSGVAGTMAANGSGVQLGTLRNIALAWVLTLPCAMCLSGFLFWSFRQLF

Sequences:

>Translated_539_residues
MVDATAPTYYQENETPERVKLDEPMGIRTLFGLLTAIALGLFFTAYSIFVDVSDAGAKITSILPFLLLGVALFIALAFEF
VNGFHDTANAVATVIYTRSLQPQVAVVWSGFFNFLGVLTSTGAVAFGIVSLLPVELILQVGSEAGFAMVFAMLIAAIIWN
LGTWWFGLPASSSHTLIGSIIGVGVTNALMRGRDGTAGVDWGKATEIGYALLLSPLVGFICSALLLLVMRALIRNPALYG
EPKGNSKPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPASHVEAFVQASEAASTVVEAKA
SGHAILGDPRPAVTAYLTQHEISEGTFPSLAVLIRDIAHQVTSYGAIAKIPLQEAGNTRNDMYLASEALRFLAKDKASEL
SAEDLTILGNYKKQLDSATKFIPPWVKVSVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAELVAMGTIFVADTY
GLPVSTTHVLSSGVAGTMAANGSGVQLGTLRNIALAWVLTLPCAMCLSGFLFWSFRQLF
>Mature_539_residues
MVDATAPTYYQENETPERVKLDEPMGIRTLFGLLTAIALGLFFTAYSIFVDVSDAGAKITSILPFLLLGVALFIALAFEF
VNGFHDTANAVATVIYTRSLQPQVAVVWSGFFNFLGVLTSTGAVAFGIVSLLPVELILQVGSEAGFAMVFAMLIAAIIWN
LGTWWFGLPASSSHTLIGSIIGVGVTNALMRGRDGTAGVDWGKATEIGYALLLSPLVGFICSALLLLVMRALIRNPALYG
EPKGNSKPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPASHVEAFVQASEAASTVVEAKA
SGHAILGDPRPAVTAYLTQHEISEGTFPSLAVLIRDIAHQVTSYGAIAKIPLQEAGNTRNDMYLASEALRFLAKDKASEL
SAEDLTILGNYKKQLDSATKFIPPWVKVSVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAELVAMGTIFVADTY
GLPVSTTHVLSSGVAGTMAANGSGVQLGTLRNIALAWVLTLPCAMCLSGFLFWSFRQLF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=476, Percent_Identity=44.1176470588235, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1789360, Length=482, Percent_Identity=43.7759336099585, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57065; Mature: 57065

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDATAPTYYQENETPERVKLDEPMGIRTLFGLLTAIALGLFFTAYSIFVDVSDAGAKIT
CCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHH
SILPFLLLGVALFIALAFEFVNGFHDTANAVATVIYTRSLQPQVAVVWSGFFNFLGVLTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
TGAVAFGIVSLLPVELILQVGSEAGFAMVFAMLIAAIIWNLGTWWFGLPASSSHTLIGSI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHH
IGVGVTNALMRGRDGTAGVDWGKATEIGYALLLSPLVGFICSALLLLVMRALIRNPALYG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPKGNSKPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPAS
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHH
HVEAFVQASEAASTVVEAKASGHAILGDPRPAVTAYLTQHEISEGTFPSLAVLIRDIAHQ
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTSYGAIAKIPLQEAGNTRNDMYLASEALRFLAKDKASELSAEDLTILGNYKKQLDSATK
HHHCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
FIPPWVKVSVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAELVAMGTIFVADTY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHEEEEEECC
GLPVSTTHVLSSGVAGTMAANGSGVQLGTLRNIALAWVLTLPCAMCLSGFLFWSFRQLF
CCCCCHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MVDATAPTYYQENETPERVKLDEPMGIRTLFGLLTAIALGLFFTAYSIFVDVSDAGAKIT
CCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHH
SILPFLLLGVALFIALAFEFVNGFHDTANAVATVIYTRSLQPQVAVVWSGFFNFLGVLTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
TGAVAFGIVSLLPVELILQVGSEAGFAMVFAMLIAAIIWNLGTWWFGLPASSSHTLIGSI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHH
IGVGVTNALMRGRDGTAGVDWGKATEIGYALLLSPLVGFICSALLLLVMRALIRNPALYG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPKGNSKPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPAS
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHH
HVEAFVQASEAASTVVEAKASGHAILGDPRPAVTAYLTQHEISEGTFPSLAVLIRDIAHQ
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTSYGAIAKIPLQEAGNTRNDMYLASEALRFLAKDKASELSAEDLTILGNYKKQLDSATK
HHHCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
FIPPWVKVSVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAELVAMGTIFVADTY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHEEEEEECC
GLPVSTTHVLSSGVAGTMAANGSGVQLGTLRNIALAWVLTLPCAMCLSGFLFWSFRQLF
CCCCCHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]