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The map label for this gene is pbpF [H]

Identifier: 182678601

GI number: 182678601

Start: 1837123

End: 1839279

Strand: Direct

Name: pbpF [H]

Synonym: Bind_1627

Alternate gene names: 182678601

Gene position: 1837123-1839279 (Clockwise)

Preceding gene: 182678598

Following gene: 182678602

Centisome position: 44.05

GC content: 57.77

Gene sequence:

>2157_bases
GTGGGCACTTCATGGTTCTCCTGGTTGAGTAAAACTGTCAGGCGCCTGCTCCTGGAGCTTGATGCATGGATTGATTCCTC
TCTTTATGACGCAAAGATCCGGTCGCGCGAGACTTATCTCGCCTATTCCGCTTTCATGGAACGGTTTCACGTCTCCGGCT
GGCGGCGTATCCTGGTGGAATGTTCCTGCGAAGGCCTGACGCTCAGCATTGCCGGGGGGCTCTTCGCCCTGTTCCTGGCC
ATTCCGGCTTTTCAGGAGACATCCGAGGACTGGCTGAAGAAGCAGGATCTGGCGATCACCTTTCTCGATCGTTCCGGCGC
GATCGTCGGCAAGCGCGGGATCAAACATGACGATTCCGTCGCCTTCGAGGAACTTCCACCGACCTTGATCCATGCCGTTC
TGGCTACCGAGGATCGCCGCTTTTTCGATCATTTCGGCATTGACATTGTCGGCACGTTCCGGGCGCTGACGGTCAATGCG
CGCGCGAGCAATGTCGTCCAGGGCGGCTCCTCAATTACCCAGCAATTGGCGAAAAACCTGTTCCTGTCCAACCAACGCAC
GCTGGAGCGCAAGATCAAGGAAGCCTATCTCGCGCTTTGGCTCGAAACACATCTGTCCAAGCGAGAAATCCTGCGTCTCT
ATCTCGACCGCGTTTATATGGGTGGCGGCACTTTTGGCGTGCAGGCAGCAGCGCAATTTTATTTTGGCAAATCGGTGCGT
GACCTGACGCTTTCGGAATCGGCCATGCTGGCCGGCCTTTTCAAAGCCCCGACCAAATATGCGCCGCATGTTAATTTGCC
GGCGGCACGCGCGCGCGCCAATGATGTCCTGACAAACATGGTCAATGCCGGCTATCTCGGCGAGGGACAAATCCATGCGG
CGTTGCGCAATCCGGCCACTCCCGTTGATCGCCGGCGCGATAACAGCCCGGATTGGTATCTCGATTATGCCTATGACGAG
GTGAAGAAACTGGTTGCGGCGGGCAAGCTTGGCGAGGAACGGGTTTTGACCGTTCGTACCGCGCTCGATTCCAATTTGCA
GAAACAGGCGGAAACGACGATCGAGGAACAATTACGCCAGCGCGGTCCTGATTATCATGTCAAACAGAGCGCCATGGTCG
TGATCGAGCCTTTGGGCGCCCTTCGTGCGATTGTCGGCGGACGTGATTATGGAGCCAGCCAGTTCAATCGCGCGACCGAC
GCATTGCGCCAACCGGGTTCATCCTTCAAACCCTTCGTGTATCTGACCGCGCTGATGAGTGGTAAATTCAAACCTTCCAC
GATCGTGCTGGATGCGCCCATCTGCATTGGCAATTGGTGCCCGCGCAATTATGGCGGCACCTATGCCGGGCATTTACCTT
TAGTGAATGCGCTGGCGCGTTCGCTGAATACGGTCGCTGTACGTCTGGCGACAGCCGTCGGCGATGGGAGCCCGAAAGCG
GGACGTGCCAAGATCATCGATACCGCGCGGCGTATGGGCCTGACGACACCGCTGACGGATACAGTTTCGTTGCCAATCGG
CGCTGCCGAGGTGACTGTCTTTGATATGGCTTCGGCCTATACAACATTCGCCAATGGCGGTTTGCGCGCACCCGCTTTCG
CGGCCATGGAAATCGCCAATAGCCGAGGCGAAATCATCTACCGTCACGATAGCGATGCCCCGGCGCCCGTGCGGATCGTC
AACGAGGATACGGTTATCGACATGGATTCCATGCTTTTGCAGGTTGTTCAGGCTGGCACCGGCCGCAAAGCGCAACTCGA
GGGCATCGATGTCGCCGGCAAGACCGGGACGACCAATGGCTACAAGGATGCCTGGTTCATCGGTTTCACCGGCAATTATG
TCGGCGCGATCTGGTTCGGCAATGACGATGACAGTCCGATGGAGAAGATGACAGGCGGCTCACTTCCCGCCGCGACTTGG
CACGAGATCATGGCCTATGCCCATCAAGGCATCGATTTGAAGCCGCTCCCCGGCCGGCCGCAACCCGTGACACCGGCCGA
GACCGTGGCGAAGTCTGGTGAAACCGCCAGCGCGGACAAAACGCAAATACGCCCAGCCTTGCTGTCACCAAATGGTATCG
AGGTCTTGAAAGCGATAGCCGACACCCTGTCGCAAGGAGACAAGCAGCGTTTGGCGGCAGGTCCGACGATACGATGA

Upstream 100 bases:

>100_bases
AAAATGACAGACCGCAGCGCGTTAACAGACGATCGACGCAAGGTTCGCACCTGTTTCCCCTGCCAAACTGTCCTCCAAGC
GTGCCGGAGAGGAGGAGCCT

Downstream 100 bases:

>100_bases
TGAGGCTCAATTTGCGAAGGGTATCGCTCCCCCCTTCATATCGAATCTCGATCTTTTCATCTTCTTGGTCAATTAATGCC
CTGAGCTTTTAGACTATGGC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MGTSWFSWLSKTVRRLLLELDAWIDSSLYDAKIRSRETYLAYSAFMERFHVSGWRRILVECSCEGLTLSIAGGLFALFLA
IPAFQETSEDWLKKQDLAITFLDRSGAIVGKRGIKHDDSVAFEELPPTLIHAVLATEDRRFFDHFGIDIVGTFRALTVNA
RASNVVQGGSSITQQLAKNLFLSNQRTLERKIKEAYLALWLETHLSKREILRLYLDRVYMGGGTFGVQAAAQFYFGKSVR
DLTLSESAMLAGLFKAPTKYAPHVNLPAARARANDVLTNMVNAGYLGEGQIHAALRNPATPVDRRRDNSPDWYLDYAYDE
VKKLVAAGKLGEERVLTVRTALDSNLQKQAETTIEEQLRQRGPDYHVKQSAMVVIEPLGALRAIVGGRDYGASQFNRATD
ALRQPGSSFKPFVYLTALMSGKFKPSTIVLDAPICIGNWCPRNYGGTYAGHLPLVNALARSLNTVAVRLATAVGDGSPKA
GRAKIIDTARRMGLTTPLTDTVSLPIGAAEVTVFDMASAYTTFANGGLRAPAFAAMEIANSRGEIIYRHDSDAPAPVRIV
NEDTVIDMDSMLLQVVQAGTGRKAQLEGIDVAGKTGTTNGYKDAWFIGFTGNYVGAIWFGNDDDSPMEKMTGGSLPAATW
HEIMAYAHQGIDLKPLPGRPQPVTPAETVAKSGETASADKTQIRPALLSPNGIEVLKAIADTLSQGDKQRLAAGPTIR

Sequences:

>Translated_718_residues
MGTSWFSWLSKTVRRLLLELDAWIDSSLYDAKIRSRETYLAYSAFMERFHVSGWRRILVECSCEGLTLSIAGGLFALFLA
IPAFQETSEDWLKKQDLAITFLDRSGAIVGKRGIKHDDSVAFEELPPTLIHAVLATEDRRFFDHFGIDIVGTFRALTVNA
RASNVVQGGSSITQQLAKNLFLSNQRTLERKIKEAYLALWLETHLSKREILRLYLDRVYMGGGTFGVQAAAQFYFGKSVR
DLTLSESAMLAGLFKAPTKYAPHVNLPAARARANDVLTNMVNAGYLGEGQIHAALRNPATPVDRRRDNSPDWYLDYAYDE
VKKLVAAGKLGEERVLTVRTALDSNLQKQAETTIEEQLRQRGPDYHVKQSAMVVIEPLGALRAIVGGRDYGASQFNRATD
ALRQPGSSFKPFVYLTALMSGKFKPSTIVLDAPICIGNWCPRNYGGTYAGHLPLVNALARSLNTVAVRLATAVGDGSPKA
GRAKIIDTARRMGLTTPLTDTVSLPIGAAEVTVFDMASAYTTFANGGLRAPAFAAMEIANSRGEIIYRHDSDAPAPVRIV
NEDTVIDMDSMLLQVVQAGTGRKAQLEGIDVAGKTGTTNGYKDAWFIGFTGNYVGAIWFGNDDDSPMEKMTGGSLPAATW
HEIMAYAHQGIDLKPLPGRPQPVTPAETVAKSGETASADKTQIRPALLSPNGIEVLKAIADTLSQGDKQRLAAGPTIR
>Mature_717_residues
GTSWFSWLSKTVRRLLLELDAWIDSSLYDAKIRSRETYLAYSAFMERFHVSGWRRILVECSCEGLTLSIAGGLFALFLAI
PAFQETSEDWLKKQDLAITFLDRSGAIVGKRGIKHDDSVAFEELPPTLIHAVLATEDRRFFDHFGIDIVGTFRALTVNAR
ASNVVQGGSSITQQLAKNLFLSNQRTLERKIKEAYLALWLETHLSKREILRLYLDRVYMGGGTFGVQAAAQFYFGKSVRD
LTLSESAMLAGLFKAPTKYAPHVNLPAARARANDVLTNMVNAGYLGEGQIHAALRNPATPVDRRRDNSPDWYLDYAYDEV
KKLVAAGKLGEERVLTVRTALDSNLQKQAETTIEEQLRQRGPDYHVKQSAMVVIEPLGALRAIVGGRDYGASQFNRATDA
LRQPGSSFKPFVYLTALMSGKFKPSTIVLDAPICIGNWCPRNYGGTYAGHLPLVNALARSLNTVAVRLATAVGDGSPKAG
RAKIIDTARRMGLTTPLTDTVSLPIGAAEVTVFDMASAYTTFANGGLRAPAFAAMEIANSRGEIIYRHDSDAPAPVRIVN
EDTVIDMDSMLLQVVQAGTGRKAQLEGIDVAGKTGTTNGYKDAWFIGFTGNYVGAIWFGNDDDSPMEKMTGGSLPAATWH
EIMAYAHQGIDLKPLPGRPQPVTPAETVAKSGETASADKTQIRPALLSPNGIEVLKAIADTLSQGDKQRLAAGPTIR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=558, Percent_Identity=32.7956989247312, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1788867, Length=520, Percent_Identity=32.1153846153846, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI87082258, Length=236, Percent_Identity=41.5254237288136, Blast_Score=182, Evalue=9e-47,
Organism=Escherichia coli, GI1789601, Length=173, Percent_Identity=40.4624277456647, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 78312; Mature: 78181

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTSWFSWLSKTVRRLLLELDAWIDSSLYDAKIRSRETYLAYSAFMERFHVSGWRRILVE
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEE
CSCEGLTLSIAGGLFALFLAIPAFQETSEDWLKKQDLAITFLDRSGAIVGKRGIKHDDSV
ECCCCEEEEHHHHHHHHHHHHCCHHCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCC
AFEELPPTLIHAVLATEDRRFFDHFGIDIVGTFRALTVNARASNVVQGGSSITQQLAKNL
CHHHCCHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHH
FLSNQRTLERKIKEAYLALWLETHLSKREILRLYLDRVYMGGGTFGVQAAAQFYFGKSVR
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
DLTLSESAMLAGLFKAPTKYAPHVNLPAARARANDVLTNMVNAGYLGEGQIHAALRNPAT
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
PVDRRRDNSPDWYLDYAYDEVKKLVAAGKLGEERVLTVRTALDSNLQKQAETTIEEQLRQ
CCHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
RGPDYHVKQSAMVVIEPLGALRAIVGGRDYGASQFNRATDALRQPGSSFKPFVYLTALMS
CCCCCEECCCCEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GKFKPSTIVLDAPICIGNWCPRNYGGTYAGHLPLVNALARSLNTVAVRLATAVGDGSPKA
CCCCCCEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRAKIIDTARRMGLTTPLTDTVSLPIGAAEVTVFDMASAYTTFANGGLRAPAFAAMEIAN
CCHHHHHHHHHCCCCCCCCCCEECCCCCHHEEEHHHHHHHHHHCCCCCCCCHHHHHHHHC
SRGEIIYRHDSDAPAPVRIVNEDTVIDMDSMLLQVVQAGTGRKAQLEGIDVAGKTGTTNG
CCCCEEEEECCCCCCCEEEECCCCEECHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCC
YKDAWFIGFTGNYVGAIWFGNDDDSPMEKMTGGSLPAATWHEIMAYAHQGIDLKPLPGRP
CCCEEEEEEECCEEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
QPVTPAETVAKSGETASADKTQIRPALLSPNGIEVLKAIADTLSQGDKQRLAAGPTIR
CCCCHHHHHHCCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure 
GTSWFSWLSKTVRRLLLELDAWIDSSLYDAKIRSRETYLAYSAFMERFHVSGWRRILVE
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEE
CSCEGLTLSIAGGLFALFLAIPAFQETSEDWLKKQDLAITFLDRSGAIVGKRGIKHDDSV
ECCCCEEEEHHHHHHHHHHHHCCHHCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCC
AFEELPPTLIHAVLATEDRRFFDHFGIDIVGTFRALTVNARASNVVQGGSSITQQLAKNL
CHHHCCHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHH
FLSNQRTLERKIKEAYLALWLETHLSKREILRLYLDRVYMGGGTFGVQAAAQFYFGKSVR
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
DLTLSESAMLAGLFKAPTKYAPHVNLPAARARANDVLTNMVNAGYLGEGQIHAALRNPAT
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
PVDRRRDNSPDWYLDYAYDEVKKLVAAGKLGEERVLTVRTALDSNLQKQAETTIEEQLRQ
CCHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
RGPDYHVKQSAMVVIEPLGALRAIVGGRDYGASQFNRATDALRQPGSSFKPFVYLTALMS
CCCCCEECCCCEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GKFKPSTIVLDAPICIGNWCPRNYGGTYAGHLPLVNALARSLNTVAVRLATAVGDGSPKA
CCCCCCEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRAKIIDTARRMGLTTPLTDTVSLPIGAAEVTVFDMASAYTTFANGGLRAPAFAAMEIAN
CCHHHHHHHHHCCCCCCCCCCEECCCCCHHEEEHHHHHHHHHHCCCCCCCCHHHHHHHHC
SRGEIIYRHDSDAPAPVRIVNEDTVIDMDSMLLQVVQAGTGRKAQLEGIDVAGKTGTTNG
CCCCEEEEECCCCCCCEEEECCCCEECHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCC
YKDAWFIGFTGNYVGAIWFGNDDDSPMEKMTGGSLPAATWHEIMAYAHQGIDLKPLPGRP
CCCEEEEEEECCEEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
QPVTPAETVAKSGETASADKTQIRPALLSPNGIEVLKAIADTLSQGDKQRLAAGPTIR
CCCCHHHHHHCCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]