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The map label for this gene is sbp [H]

Identifier: 182678547

GI number: 182678547

Start: 1775447

End: 1776481

Strand: Reverse

Name: sbp [H]

Synonym: Bind_1572

Alternate gene names: 182678547

Gene position: 1776481-1775447 (Counterclockwise)

Preceding gene: 182678548

Following gene: 182678546

Centisome position: 42.6

GC content: 58.84

Gene sequence:

>1035_bases
ATGTTCCAATCCCTGACCCATCGGCGCCGTCAATCATTGAGAGCCGCGTTCATCCTCCTCGGCTCCTTCTCCGCAGCGCT
CTCAGCGCAAGCGGAAACCGCTTTGCTGAATGTGTCTTATGATCCAACGCGTGAGCTCTACCGCGCCATCAACGAGGCTT
TTGTTCAGGATTGGAAGGCCAAGACGGGCGAGACTGTCACGGTTCGCGCCTCGCATGGGGGCTCCGGCGCCCAGGCTCGC
GCCGTTATCGACGGACTCGATGCTGATGTCGTCACGCTGGCGCTTGCCGCTGATATCGACGCCATCGCCGCCAAGAGCGG
TAAAATTCCCGCCGATTGGCAGAAACGTCTACCGCATAATTCCACGCCCTACACATCGACGATCGTCTTTGTCGTCCGCA
AGGGCAATCCCAAGGGGATCAAAGATTGGAATGATCTCGTCAAGCCGGGCATCTCTGTCATTACGCCCAATCCCAAAACC
TCAGGCGGCGCCCGCTGGAACTATCTGGCGGCCTGGGGCTATGCCAATAAGATTTTCGGAGGGGATGACGCCAAGATCCG
CGACTTTATCGGCGCGCTTTATCGCAACACGCCGGTGCTCGACACCGGCGCGCGCGGCTCGACGATCAGCTTCGCCCAGC
GCGGTCTTGGGGATGTCCTGATCTCCTGGGAGAATGACGCTTTTCTAGCCAGTGAGGAATTCGGCAAGGATAAATTCGAT
GTTGTCGTGCCGTCGGTCTCCGTCCTCGCCGAACCTCCCGTTGCCCTGGTCGATGGCAATGTCGACCACAAGGGAACCCG
CAAGGTGGCGGAAGGCTATCTCGATTTTCTCTATCAGCCGAAGGCGCAGGCCATCATCGCCAAGAATTACTATCACCCGA
TCAATCCAGCAGCTGCCGCGCCGGAAGATCTCGCTCGCCTCGCCAAACCGGCGACGCTCGTGACGGTCGATCAGGATTTC
GGCGGTTGGACCAAGGTCCAATCGACCCATTTCGCGGATGGCGGCGTGTTCGATCAGATCTACACCAAGCATTGA

Upstream 100 bases:

>100_bases
ATAATTCATTGTTCTTCAATAATAAAATACAAAAAGACGGCAAGCTTGACAGATTTCTATATTTTTATAGATTTACTGGA
TTAATCAACCTGGGTGACTC

Downstream 100 bases:

>100_bases
GACTTGAACGGAAAGAACGCTGTGAAGCATGATGGAGGTGTCCCCCATCATGCTTCACGAGAAGATCATTCCATAAGCCG
AAGAAAATAGAGCAATATTG

Product: sulfate ABC transporter periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MFQSLTHRRRQSLRAAFILLGSFSAALSAQAETALLNVSYDPTRELYRAINEAFVQDWKAKTGETVTVRASHGGSGAQAR
AVIDGLDADVVTLALAADIDAIAAKSGKIPADWQKRLPHNSTPYTSTIVFVVRKGNPKGIKDWNDLVKPGISVITPNPKT
SGGARWNYLAAWGYANKIFGGDDAKIRDFIGALYRNTPVLDTGARGSTISFAQRGLGDVLISWENDAFLASEEFGKDKFD
VVVPSVSVLAEPPVALVDGNVDHKGTRKVAEGYLDFLYQPKAQAIIAKNYYHPINPAAAAPEDLARLAKPATLVTVDQDF
GGWTKVQSTHFADGGVFDQIYTKH

Sequences:

>Translated_344_residues
MFQSLTHRRRQSLRAAFILLGSFSAALSAQAETALLNVSYDPTRELYRAINEAFVQDWKAKTGETVTVRASHGGSGAQAR
AVIDGLDADVVTLALAADIDAIAAKSGKIPADWQKRLPHNSTPYTSTIVFVVRKGNPKGIKDWNDLVKPGISVITPNPKT
SGGARWNYLAAWGYANKIFGGDDAKIRDFIGALYRNTPVLDTGARGSTISFAQRGLGDVLISWENDAFLASEEFGKDKFD
VVVPSVSVLAEPPVALVDGNVDHKGTRKVAEGYLDFLYQPKAQAIIAKNYYHPINPAAAAPEDLARLAKPATLVTVDQDF
GGWTKVQSTHFADGGVFDQIYTKH
>Mature_344_residues
MFQSLTHRRRQSLRAAFILLGSFSAALSAQAETALLNVSYDPTRELYRAINEAFVQDWKAKTGETVTVRASHGGSGAQAR
AVIDGLDADVVTLALAADIDAIAAKSGKIPADWQKRLPHNSTPYTSTIVFVVRKGNPKGIKDWNDLVKPGISVITPNPKT
SGGARWNYLAAWGYANKIFGGDDAKIRDFIGALYRNTPVLDTGARGSTISFAQRGLGDVLISWENDAFLASEEFGKDKFD
VVVPSVSVLAEPPVALVDGNVDHKGTRKVAEGYLDFLYQPKAQAIIAKNYYHPINPAAAAPEDLARLAKPATLVTVDQDF
GGWTKVQSTHFADGGVFDQIYTKH

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=317, Percent_Identity=62.1451104100946, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=49.0322580645161, Blast_Score=297, Evalue=6e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37218; Mature: 37218

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00757 PROK_SULFATE_BIND_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQSLTHRRRQSLRAAFILLGSFSAALSAQAETALLNVSYDPTRELYRAINEAFVQDWKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
KTGETVTVRASHGGSGAQARAVIDGLDADVVTLALAADIDAIAAKSGKIPADWQKRLPHN
CCCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHCCCCCCCHHHHHCCCC
STPYTSTIVFVVRKGNPKGIKDWNDLVKPGISVITPNPKTSGGARWNYLAAWGYANKIFG
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCCCCCC
GDDAKIRDFIGALYRNTPVLDTGARGSTISFAQRGLGDVLISWENDAFLASEEFGKDKFD
CCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHCCCCEEEEEECCCCEEEHHHCCCCCEE
VVVPSVSVLAEPPVALVDGNVDHKGTRKVAEGYLDFLYQPKAQAIIAKNYYHPINPAAAA
EEECCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCC
PEDLARLAKPATLVTVDQDFGGWTKVQSTHFADGGVFDQIYTKH
HHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MFQSLTHRRRQSLRAAFILLGSFSAALSAQAETALLNVSYDPTRELYRAINEAFVQDWKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
KTGETVTVRASHGGSGAQARAVIDGLDADVVTLALAADIDAIAAKSGKIPADWQKRLPHN
CCCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHCCCCCCCHHHHHCCCC
STPYTSTIVFVVRKGNPKGIKDWNDLVKPGISVITPNPKTSGGARWNYLAAWGYANKIFG
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCCCCCC
GDDAKIRDFIGALYRNTPVLDTGARGSTISFAQRGLGDVLISWENDAFLASEEFGKDKFD
CCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHCCCCEEEEEECCCCEEEHHHCCCCCEE
VVVPSVSVLAEPPVALVDGNVDHKGTRKVAEGYLDFLYQPKAQAIIAKNYYHPINPAAAA
EEECCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCC
PEDLARLAKPATLVTVDQDFGGWTKVQSTHFADGGVFDQIYTKH
HHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]