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The map label for this gene is cobB1 [H]

Identifier: 182678528

GI number: 182678528

Start: 1754022

End: 1754783

Strand: Reverse

Name: cobB1 [H]

Synonym: Bind_1553

Alternate gene names: 182678528

Gene position: 1754783-1754022 (Counterclockwise)

Preceding gene: 182678532

Following gene: 182678527

Centisome position: 42.08

GC content: 57.61

Gene sequence:

>762_bases
ATGGTCATCCTGAATCAGATTGGCGAAGCGCGGACGGCCTTGCTCGAATTGATCGAACAGTCCGACAATATCGTCGCCTT
TACCGGCGCCGGTATTTCAACCGAATGCGGCGTCCCGGATTTTCGTTCCAAAGATTCTCCGTGGATGCGTTATAAGCCGA
TCGAATTCAATCTCTTCCTGTCCGATGTCTTGATGCGTGAGGAAGCCTGGCGGCGGAAATTCGCCCTCGACGATATCTAT
TCCATGGCGCAGCCCGGGCGCGGCCATTATGCCCTCGCCAATCTGGTCCAACAGGGCAAAATCTCAGCCATCATCACGCA
GAATATCGATAATCTCCACCAGATGTCTGGCGTCGACGACGAGCACATCATCGAATTGCACGGCAATGGCACTTATGCCA
CCTGCCTGTCCTGCGGGCTTCGCCATGAACTGGCCAACGTGCGGCACGATTTTGAGACGACCGGGGCGGCGCCGGAGTGC
AGGTCCTGTGGCGGTCCGGTCAAATCCGCGACCATTTCCTTCGGCCAATCCATGCCTGAGGAGGCCATGCAGCGCGCCCA
TGACGTGACTCGGGCCTGTGATTTGTTTCTGGCCATCGGTTCCTCACTCGTCGTCTACCCGGCCGCCGCCTTTCCACTCC
TCGCGGCGCAAAACAACGCGCGTCTCGTGATCTTGAATGGGGAAGCGACACCGCTCGATTCCGAGGCGGATCTGATCCTG
CGCGGCGATATTGGTGATATTCTCGAACCCTTCTCGAGCTAA

Upstream 100 bases:

>100_bases
ATGAACTTTTTCCAAAACCAGTCCCGGCGATGCCACTGGACCGTGACAAAAAGAGAGAGTTCCGGTCCCTGAACCGTGAA
GCAGGCGTGGAGAAGGTGGG

Downstream 100 bases:

>100_bases
GCCGCGTCTTCGCTTCCCCATTCATTTTACCCGGGTCGAACAAGAGCGGTTCTGAATCACTTTCTCCCGAATCGGGAGAA
TTCAGGCTTTTCCTTCCGCC

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 1 [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MVILNQIGEARTALLELIEQSDNIVAFTGAGISTECGVPDFRSKDSPWMRYKPIEFNLFLSDVLMREEAWRRKFALDDIY
SMAQPGRGHYALANLVQQGKISAIITQNIDNLHQMSGVDDEHIIELHGNGTYATCLSCGLRHELANVRHDFETTGAAPEC
RSCGGPVKSATISFGQSMPEEAMQRAHDVTRACDLFLAIGSSLVVYPAAAFPLLAAQNNARLVILNGEATPLDSEADLIL
RGDIGDILEPFSS

Sequences:

>Translated_253_residues
MVILNQIGEARTALLELIEQSDNIVAFTGAGISTECGVPDFRSKDSPWMRYKPIEFNLFLSDVLMREEAWRRKFALDDIY
SMAQPGRGHYALANLVQQGKISAIITQNIDNLHQMSGVDDEHIIELHGNGTYATCLSCGLRHELANVRHDFETTGAAPEC
RSCGGPVKSATISFGQSMPEEAMQRAHDVTRACDLFLAIGSSLVVYPAAAFPLLAAQNNARLVILNGEATPLDSEADLIL
RGDIGDILEPFSS
>Mature_253_residues
MVILNQIGEARTALLELIEQSDNIVAFTGAGISTECGVPDFRSKDSPWMRYKPIEFNLFLSDVLMREEAWRRKFALDDIY
SMAQPGRGHYALANLVQQGKISAIITQNIDNLHQMSGVDDEHIIELHGNGTYATCLSCGLRHELANVRHDFETTGAAPEC
RSCGGPVKSATISFGQSMPEEAMQRAHDVTRACDLFLAIGSSLVVYPAAAFPLLAAQNNARLVILNGEATPLDSEADLIL
RGDIGDILEPFSS

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=269, Percent_Identity=31.2267657992565, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI300797577, Length=243, Percent_Identity=30.4526748971193, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=28.6919831223629, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI6912664, Length=255, Percent_Identity=27.4509803921569, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI300797597, Length=235, Percent_Identity=30.6382978723404, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI7657575, Length=229, Percent_Identity=30.5676855895196, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI13787215, Length=238, Percent_Identity=28.1512605042017, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI13775602, Length=220, Percent_Identity=29.5454545454545, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI6912660, Length=221, Percent_Identity=30.7692307692308, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI13775600, Length=220, Percent_Identity=29.5454545454545, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI63054862, Length=216, Percent_Identity=31.0185185185185, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI7706712, Length=249, Percent_Identity=29.3172690763052, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI300797705, Length=194, Percent_Identity=29.3814432989691, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI215982798, Length=164, Percent_Identity=30.4878048780488, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990482, Length=268, Percent_Identity=28.3582089552239, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17541892, Length=240, Percent_Identity=30.4166666666667, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=265, Percent_Identity=29.811320754717, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=270, Percent_Identity=28.1481481481481, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17505510, Length=232, Percent_Identity=26.2931034482759, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6325242, Length=222, Percent_Identity=29.2792792792793, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320163, Length=277, Percent_Identity=24.187725631769, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324504, Length=273, Percent_Identity=23.0769230769231, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571445, Length=268, Percent_Identity=28.7313432835821, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24645650, Length=254, Percent_Identity=27.9527559055118, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17137536, Length=228, Percent_Identity=28.0701754385965, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648389, Length=207, Percent_Identity=29.4685990338164, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571443, Length=220, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571441, Length=220, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27609; Mature: 27609

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILNQIGEARTALLELIEQSDNIVAFTGAGISTECGVPDFRSKDSPWMRYKPIEFNLFL
CEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCEEEHHHH
SDVLMREEAWRRKFALDDIYSMAQPGRGHYALANLVQQGKISAIITQNIDNLHQMSGVDD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEHHCHHHHHHHCCCCC
EHIIELHGNGTYATCLSCGLRHELANVRHDFETTGAAPECRSCGGPVKSATISFGQSMPE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCCCCCH
EAMQRAHDVTRACDLFLAIGSSLVVYPAAAFPLLAAQNNARLVILNGEATPLDSEADLIL
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHEEECCCCEEEEECCCCCCCCCCCCEEE
RGDIGDILEPFSS
ECCHHHHHHHCCC
>Mature Secondary Structure
MVILNQIGEARTALLELIEQSDNIVAFTGAGISTECGVPDFRSKDSPWMRYKPIEFNLFL
CEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCEEEHHHH
SDVLMREEAWRRKFALDDIYSMAQPGRGHYALANLVQQGKISAIITQNIDNLHQMSGVDD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEHHCHHHHHHHCCCCC
EHIIELHGNGTYATCLSCGLRHELANVRHDFETTGAAPECRSCGGPVKSATISFGQSMPE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCCCCCH
EAMQRAHDVTRACDLFLAIGSSLVVYPAAAFPLLAAQNNARLVILNGEATPLDSEADLIL
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHEEECCCCEEEEECCCCCCCCCCCCEEE
RGDIGDILEPFSS
ECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]