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The map label for this gene is ntrX [H]

Identifier: 182678497

GI number: 182678497

Start: 1723334

End: 1724701

Strand: Direct

Name: ntrX [H]

Synonym: Bind_1521

Alternate gene names: 182678497

Gene position: 1723334-1724701 (Clockwise)

Preceding gene: 182678496

Following gene: 182678498

Centisome position: 41.33

GC content: 59.94

Gene sequence:

>1368_bases
ATGGGCAGCGACATTCTCATCGTAGACGACGAAACGGACATTCGCGAAATCGTTTCCGGCATCCTTTCCGACGAAGGCTA
CGGAACCCGTACCGCGAAAAACTCCGACGAGGCCCTGAAGGAAATCGAGGCCCGCCAGCCGGGTCTCATCTTTCTCGACA
TCTGGCTGCAGGGGTCGCGACTCGATGGATTGCAATTGCTGCAGGTCATCAAGGACCTGAATCCGGCGCTGCCGATCGTC
ATGATCTCTGGCCATGGCAATATCGAGACGGCGGTTTCGGCCATCAAGCTCGGCGCCTATGATTTCATCGAAAAGCCGTT
CAAGGCTGATCGCCTGGTCCTTGTTGCCGAACATGCGCTTGAAGCCACCCGCTTGAAACGCGAGGTGAATGACCTGCGCA
CGCGCAGCGGTGTCACCAATAAGATCATTGGCAAGTCGAGCACCATCAATCAACTGCGGCAGACGATCGACCGCGTCGCC
GCTTCGAATTCCCGTATTCTCATTACCGGCGCGCCGGGGGTGGGCAAGGAATTGGCCGCTCGTTCGATTCACGCGGCGTC
GAGCCGCGCCAATGGCCCCTTCATCGTGCTCAATGCGGCGACAATCGCGCCCGAGACGATGGAAATCGAATTGTTTGGCA
CCGAGGGCGGCGATGGACATGGCCGCAAGATCGGTGCTTTGGAGGAGGCCCACGGCGGCACCCTTTATCTCGACGAGATC
GCCGACATGCCGCGCGAGACGCAGGGCAAGATTCTGCGTGTGCTGGTCGAACAGAATTTCCAAAGGGTCAATGGCACCAC
GCGGGTGCATGTCGATGTCCGCATCATTTCCTCGAGCGCGCGCGATCTCACGGCCGCGATAGCTGATGGCGTCCTGCGCG
AGGATCTGTTCCACCGCTTGTCCGTGGTGCCGGTCCGGGTGCCTTCGCTTGCCGAGCGCCGGGAGGACATCGCCGAGCTC
ATCGACTATTTCATGGATCAGGTGTCCACCATGACCGGCATGCCGCGCCGTGCCATTGCCGACGACGCCATGGCCGTCCT
GCAATCGCATGACTGGCCCGGTAATATCCGGCAATTGCGCAATAATGTGGAACGGCTGATGATCCTCGCCGCGGGTGATC
CCTCTATTCCCGTTACGGCCGATATGCTGCCTTCGGAAATCGGAGCGCTCGTGCCCGCCATGCCGAATGGTTCGGGCGGC
GAGAAGCTTCTGGGGCTTCCCCTGCGCGAAGCGCGGGAAATGTTCGAGCGCGAATATCTCGTCGCTCAGATCAATCGGTT
CGGCGGCAATATTTCGCGTACCGCCGAATTCATCGGCATGGAACGCTCCGCTTTGCACCGCAAGCTGAAATCCCTGCGGA
TCGATTAG

Upstream 100 bases:

>100_bases
CATAGCGTGCTGTAGACTCTGATCGGCTCTTTCTAGCGCATCCTCGCCAGCGCATCCCCGAACCAATCTTTCCAGACCCC
GAGCAACCAGAAATGGGCAC

Downstream 100 bases:

>100_bases
TATCCATCCTGTAAGGACGTCTGCGCGCGAGGAAACAGGCGGAGGAGGTGTCATGAACCTGCAAACGCTTGCGATGGAAC
CACCACCCTCGCGCCGAGCC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MGSDILIVDDETDIREIVSGILSDEGYGTRTAKNSDEALKEIEARQPGLIFLDIWLQGSRLDGLQLLQVIKDLNPALPIV
MISGHGNIETAVSAIKLGAYDFIEKPFKADRLVLVAEHALEATRLKREVNDLRTRSGVTNKIIGKSSTINQLRQTIDRVA
ASNSRILITGAPGVGKELAARSIHAASSRANGPFIVLNAATIAPETMEIELFGTEGGDGHGRKIGALEEAHGGTLYLDEI
ADMPRETQGKILRVLVEQNFQRVNGTTRVHVDVRIISSSARDLTAAIADGVLREDLFHRLSVVPVRVPSLAERREDIAEL
IDYFMDQVSTMTGMPRRAIADDAMAVLQSHDWPGNIRQLRNNVERLMILAAGDPSIPVTADMLPSEIGALVPAMPNGSGG
EKLLGLPLREAREMFEREYLVAQINRFGGNISRTAEFIGMERSALHRKLKSLRID

Sequences:

>Translated_455_residues
MGSDILIVDDETDIREIVSGILSDEGYGTRTAKNSDEALKEIEARQPGLIFLDIWLQGSRLDGLQLLQVIKDLNPALPIV
MISGHGNIETAVSAIKLGAYDFIEKPFKADRLVLVAEHALEATRLKREVNDLRTRSGVTNKIIGKSSTINQLRQTIDRVA
ASNSRILITGAPGVGKELAARSIHAASSRANGPFIVLNAATIAPETMEIELFGTEGGDGHGRKIGALEEAHGGTLYLDEI
ADMPRETQGKILRVLVEQNFQRVNGTTRVHVDVRIISSSARDLTAAIADGVLREDLFHRLSVVPVRVPSLAERREDIAEL
IDYFMDQVSTMTGMPRRAIADDAMAVLQSHDWPGNIRQLRNNVERLMILAAGDPSIPVTADMLPSEIGALVPAMPNGSGG
EKLLGLPLREAREMFEREYLVAQINRFGGNISRTAEFIGMERSALHRKLKSLRID
>Mature_454_residues
GSDILIVDDETDIREIVSGILSDEGYGTRTAKNSDEALKEIEARQPGLIFLDIWLQGSRLDGLQLLQVIKDLNPALPIVM
ISGHGNIETAVSAIKLGAYDFIEKPFKADRLVLVAEHALEATRLKREVNDLRTRSGVTNKIIGKSSTINQLRQTIDRVAA
SNSRILITGAPGVGKELAARSIHAASSRANGPFIVLNAATIAPETMEIELFGTEGGDGHGRKIGALEEAHGGTLYLDEIA
DMPRETQGKILRVLVEQNFQRVNGTTRVHVDVRIISSSARDLTAAIADGVLREDLFHRLSVVPVRVPSLAERREDIAELI
DYFMDQVSTMTGMPRRAIADDAMAVLQSHDWPGNIRQLRNNVERLMILAAGDPSIPVTADMLPSEIGALVPAMPNGSGGE
KLLGLPLREAREMFEREYLVAQINRFGGNISRTAEFIGMERSALHRKLKSLRID

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=35.4700854700855, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=35.0762527233115, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=36.3636363636364, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=34.2105263157895, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1786524, Length=340, Percent_Identity=36.4705882352941, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789233, Length=231, Percent_Identity=40.6926406926407, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=36.5030674846626, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=41.7021276595745, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=32.9341317365269, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=34.0836012861736, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=32.9230769230769, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=31.5589353612167, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=28.8590604026846, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49765; Mature: 49634

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDILIVDDETDIREIVSGILSDEGYGTRTAKNSDEALKEIEARQPGLIFLDIWLQGSR
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLQVIKDLNPALPIVMISGHGNIETAVSAIKLGAYDFIEKPFKADRLVLVAEHAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
EATRLKREVNDLRTRSGVTNKIIGKSSTINQLRQTIDRVAASNSRILITGAPGVGKELAA
HHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RSIHAASSRANGPFIVLNAATIAPETMEIELFGTEGGDGHGRKIGALEEAHGGTLYLDEI
HHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCEECCHHHCCCCEEEEHHH
ADMPRETQGKILRVLVEQNFQRVNGTTRVHVDVRIISSSARDLTAAIADGVLREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
SVVPVRVPSLAERREDIAELIDYFMDQVSTMTGMPRRAIADDAMAVLQSHDWPGNIRQLR
HCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
NNVERLMILAAGDPSIPVTADMLPSEIGALVPAMPNGSGGEKLLGLPLREAREMFEREYL
HHHHHEEEEECCCCCCCEEHHHCHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
VAQINRFGGNISRTAEFIGMERSALHRKLKSLRID
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GSDILIVDDETDIREIVSGILSDEGYGTRTAKNSDEALKEIEARQPGLIFLDIWLQGSR
CCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLQVIKDLNPALPIVMISGHGNIETAVSAIKLGAYDFIEKPFKADRLVLVAEHAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
EATRLKREVNDLRTRSGVTNKIIGKSSTINQLRQTIDRVAASNSRILITGAPGVGKELAA
HHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RSIHAASSRANGPFIVLNAATIAPETMEIELFGTEGGDGHGRKIGALEEAHGGTLYLDEI
HHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCEECCHHHCCCCEEEEHHH
ADMPRETQGKILRVLVEQNFQRVNGTTRVHVDVRIISSSARDLTAAIADGVLREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
SVVPVRVPSLAERREDIAELIDYFMDQVSTMTGMPRRAIADDAMAVLQSHDWPGNIRQLR
HCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
NNVERLMILAAGDPSIPVTADMLPSEIGALVPAMPNGSGGEKLLGLPLREAREMFEREYL
HHHHHEEEEECCCCCCCEEHHHCHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
VAQINRFGGNISRTAEFIGMERSALHRKLKSLRID
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]