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The map label for this gene is ispDF [H]

Identifier: 182678492

GI number: 182678492

Start: 1715484

End: 1716710

Strand: Reverse

Name: ispDF [H]

Synonym: Bind_1516

Alternate gene names: 182678492

Gene position: 1716710-1715484 (Counterclockwise)

Preceding gene: 182678503

Following gene: 182678491

Centisome position: 41.17

GC content: 62.51

Gene sequence:

>1227_bases
ATGCATCGAAACAATGAAGTGGCGGTCCTTGATGTGGCGATCCTGGTTGTCGCGGCAGGGCGCGGTGTGCGGGCTGGCGG
CGGCTTGCCCAAGCAATATCGCGAGGTCGCTGGCCGCAGCGTCTTGGCCCACACAATCCAAGCCTTGGCCGAGGCTTTGC
CGGAGGCGCTCATTCTGCCGGTCATCCATGCCGATGATGAAGCGCTTTTCGCCGCAGCGCGGGCCGAGGTTGCAAATGTC
GATTCGCGCCAGCTCGCCGCTCCCGTCTTTGGCGGTGCGACGCGGCAGCAAAGCGTCAAGGCCGGGCTGGAAGCTTTGGC
AGCACGCGATAAGGCACCCGACATCGTTCTAATTCATGATGCGGCACGGCCTTTTTCGAGCCCGGCCCTCATTCGCCGCG
CGGTTGAGGCCGCGCAAAGCCGAGGCGCGGCCATTCCGGGTGTTCCGCTGACCGATACGGTGAAACAGATCGATCTCCAG
GGTGAGGACGATTCGCTGATTATCGCCACACCCGCCCGCCAGAGCCTGCGGGCGGTGCAGACACCCCAAGCTTTTCGTTT
CGACCTGATTCTGTCAGGCCATCGCCAAGCCGCCGAAGCTGGCGCCGATGATTTGACGGATGATGCGGCCATCATCGAAT
GGATGGGACAGCAGGTCCATATTTTCGAAGGGGATCCCGACAATATGAAGATCACCACTCCGCAGGATTTCGTCGCGGCT
GAAGCCCGCCTGCTCCGCGATCTGCCCGATATTCGGACCGGCCAGGGCTATGACGTCCATGCGCTTGGCGCAGGCGATCA
TGTCTGGCTCGGCGGGGTGAAAATTCCCCATGATCGCGGCCTGATCGGCCATTCTGACGCCGATGTCCTCTCCCATGCGA
TCACCGATGCTTTGTTCGGTGCCTTAGCGGAAGGGGATATCGGCAGTCATTTCCCCCCGTCCGATCCGCAATGGAAAGGC
GCCGATTCGGCGATCTTCCTGCGCGAGGCGGCCAATCGCGTGCGCGAGCGCGGCGGCATGATTGCCCATATCGATGCAAC
AGTAGTCTGCGAAAGGCCGAAAGTCGGCCCGCATCGCGACGCCATCCGCGAACGTCTCGCCGAAATCACCGGCTGCCCGA
TCGGGCGCGTTGCCGTCAAAGCGACGACCTCGGAAAAGCTTGGTTTCACCGGCCGCGAGGAAGGCATCGCGGCTTTGGCG
CTCGCTACCGTCAGGCTGCCGCTTTAA

Upstream 100 bases:

>100_bases
GACAAGGATCGGTCGGCCTCTCATTTTTTGCTTGAAACCTGTGCCGTTCCTGTCCTTGGGTTCGCACTCTTCTCACATTT
CTAGGCGATCAACGATCCTC

Downstream 100 bases:

>100_bases
ACGGTAGCTTGTCCCATACCATGCTTTCCGAATAGGCTAAGATTGATGAAACACGCAATCGGATAGCATGGTACAAATGC
ACGAAAATAACGATCTTTCA

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHRNNEVAVLDVAILVVAAGRGVRAGGGLPKQYREVAGRSVLAHTIQALAEALPEALILPVIHADDEALFAAARAEVANV
DSRQLAAPVFGGATRQQSVKAGLEALAARDKAPDIVLIHDAARPFSSPALIRRAVEAAQSRGAAIPGVPLTDTVKQIDLQ
GEDDSLIIATPARQSLRAVQTPQAFRFDLILSGHRQAAEAGADDLTDDAAIIEWMGQQVHIFEGDPDNMKITTPQDFVAA
EARLLRDLPDIRTGQGYDVHALGAGDHVWLGGVKIPHDRGLIGHSDADVLSHAITDALFGALAEGDIGSHFPPSDPQWKG
ADSAIFLREAANRVRERGGMIAHIDATVVCERPKVGPHRDAIRERLAEITGCPIGRVAVKATTSEKLGFTGREEGIAALA
LATVRLPL

Sequences:

>Translated_408_residues
MHRNNEVAVLDVAILVVAAGRGVRAGGGLPKQYREVAGRSVLAHTIQALAEALPEALILPVIHADDEALFAAARAEVANV
DSRQLAAPVFGGATRQQSVKAGLEALAARDKAPDIVLIHDAARPFSSPALIRRAVEAAQSRGAAIPGVPLTDTVKQIDLQ
GEDDSLIIATPARQSLRAVQTPQAFRFDLILSGHRQAAEAGADDLTDDAAIIEWMGQQVHIFEGDPDNMKITTPQDFVAA
EARLLRDLPDIRTGQGYDVHALGAGDHVWLGGVKIPHDRGLIGHSDADVLSHAITDALFGALAEGDIGSHFPPSDPQWKG
ADSAIFLREAANRVRERGGMIAHIDATVVCERPKVGPHRDAIRERLAEITGCPIGRVAVKATTSEKLGFTGREEGIAALA
LATVRLPL
>Mature_408_residues
MHRNNEVAVLDVAILVVAAGRGVRAGGGLPKQYREVAGRSVLAHTIQALAEALPEALILPVIHADDEALFAAARAEVANV
DSRQLAAPVFGGATRQQSVKAGLEALAARDKAPDIVLIHDAARPFSSPALIRRAVEAAQSRGAAIPGVPLTDTVKQIDLQ
GEDDSLIIATPARQSLRAVQTPQAFRFDLILSGHRQAAEAGADDLTDDAAIIEWMGQQVHIFEGDPDNMKITTPQDFVAA
EARLLRDLPDIRTGQGYDVHALGAGDHVWLGGVKIPHDRGLIGHSDADVLSHAITDALFGALAEGDIGSHFPPSDPQWKG
ADSAIFLREAANRVRERGGMIAHIDATVVCERPKVGPHRDAIRERLAEITGCPIGRVAVKATTSEKLGFTGREEGIAALA
LATVRLPL

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=51.2987012987013, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789104, Length=244, Percent_Identity=33.1967213114754, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 43203; Mature: 43203

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRNNEVAVLDVAILVVAAGRGVRAGGGLPKQYREVAGRSVLAHTIQALAEALPEALILP
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
VIHADDEALFAAARAEVANVDSRQLAAPVFGGATRQQSVKAGLEALAARDKAPDIVLIHD
EEECCCHHHHHHHHHHHHCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
AARPFSSPALIRRAVEAAQSRGAAIPGVPLTDTVKQIDLQGEDDSLIIATPARQSLRAVQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCCEEEECCCHHHHHHHC
TPQAFRFDLILSGHRQAAEAGADDLTDDAAIIEWMGQQVHIFEGDPDNMKITTPQDFVAA
CCCCEEEEEEECCCHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEECCHHHHHH
EARLLRDLPDIRTGQGYDVHALGAGDHVWLGGVKIPHDRGLIGHSDADVLSHAITDALFG
HHHHHHHCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHH
ALAEGDIGSHFPPSDPQWKGADSAIFLREAANRVRERGGMIAHIDATVVCERPKVGPHRD
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECEEEEECCCCCCCCHH
AIRERLAEITGCPIGRVAVKATTSEKLGFTGREEGIAALALATVRLPL
HHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCHHHHHHEEHHHHCCCC
>Mature Secondary Structure
MHRNNEVAVLDVAILVVAAGRGVRAGGGLPKQYREVAGRSVLAHTIQALAEALPEALILP
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
VIHADDEALFAAARAEVANVDSRQLAAPVFGGATRQQSVKAGLEALAARDKAPDIVLIHD
EEECCCHHHHHHHHHHHHCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
AARPFSSPALIRRAVEAAQSRGAAIPGVPLTDTVKQIDLQGEDDSLIIATPARQSLRAVQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCCEEEECCCHHHHHHHC
TPQAFRFDLILSGHRQAAEAGADDLTDDAAIIEWMGQQVHIFEGDPDNMKITTPQDFVAA
CCCCEEEEEEECCCHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEECCHHHHHH
EARLLRDLPDIRTGQGYDVHALGAGDHVWLGGVKIPHDRGLIGHSDADVLSHAITDALFG
HHHHHHHCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHH
ALAEGDIGSHFPPSDPQWKGADSAIFLREAANRVRERGGMIAHIDATVVCERPKVGPHRD
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECEEEEECCCCCCCCHH
AIRERLAEITGCPIGRVAVKATTSEKLGFTGREEGIAALALATVRLPL
HHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCHHHHHHEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA