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The map label for this gene is lipA

Identifier: 182678485

GI number: 182678485

Start: 1710367

End: 1711344

Strand: Direct

Name: lipA

Synonym: Bind_1509

Alternate gene names: 182678485

Gene position: 1710367-1711344 (Clockwise)

Preceding gene: 182678484

Following gene: 182678488

Centisome position: 41.01

GC content: 60.94

Gene sequence:

>978_bases
ATGCCGCCTTTAGCCGATGCTTCTACCGAGACTTTGTCCCCGGCCGAGCAAGCGGCGGTGCGCCATCCGGAAAAAGCGCA
TCGTCCCGACCAGCCCATTGCCCGCAAGCCCGATTGGATCCGCGTCAAGGCACCAGGCTCGCCGGAATGGGTCGAAACGC
AGAAAATCGTCAAGGAGCATAAGCTCGTCACGGTCTGCGAGGAAGCCGGCTGCCCGAATATCGGCGAATGCTGGGCGAAA
AAGCACGCGACCTTCATGATCATGGGTGACACGTGCACGCGGGCTTGCGCCTTCTGCAATGTCAAGACGGGCCTGCCGAA
CGGGCTTGATCCGCATGAGCCGGCCCATATCGCCGATGCGGTGCAGAAGCTTGGGCTCTCGCATGTGGTCATTACCTCGG
TCGATCGCGATGATCTCGCCGATGGCGGCGCCGAGCATTTCGCGCAAGTCATTCGCGCGATCCGCGAGGCGAGCCCCAAA
ACCACGATCGAAGTGCTGACCCCGGATTTCCTGCGCAAGGATGGAGCGCTCGAAATTGTGGTCGCGGCGCGGCCCGATGT
CTTCAACCATAATCTCGAGACGGTGCCCGCGAAATATCTGAGCGTGCGGCCCGGCGCGCGCTATTTCCATTCGATCCGCC
TGCTGCAGCGCGTGAAGGAACTCGATCCGACCATCTTCACCAAATCCGGCATTATGGTCGGCCTTGGCGAGACCCGTAAC
GAAGTGCTCCAATTGATGGATGATCTGCGCACGGCGGATGTGGATTTCCTGACGATCGGCCAATATCTCCAGCCGACCCG
CAAACATCATGCGGTTGAGGCTTTCATCACGCCGGATGAATTTGCGGCCTATGCCGAAGTCGCCAATGCCAAGGGCTTTC
TGCTCGTCTCAGCCTCGCCCTTGACCCGTTCCTCGCATCATGCTGGGGAGGATTTTGCGCGGTTGAAGGCGGCGCGGCTC
GAGCGGCTGGGGCGGTGA

Upstream 100 bases:

>100_bases
GTGCTTGATGCTTACGGCCGAGTGATCCATATGTAGAAAATCAAAGGGGCGCGAAAAGCGCCCCTTGTTCAATGAATAAC
GAAATAAGGGAACGTCGCCG

Downstream 100 bases:

>100_bases
GTAAGGCTTGCCATCCGCGCCGGGGTCAAGGCTTTTTGCCAAGGCGCGGATCATAAAGCCATTTGCGGACCTTGGTGGCC
GTAATCCGCGCAAGGGCTGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPPLADASTETLSPAEQAAVRHPEKAHRPDQPIARKPDWIRVKAPGSPEWVETQKIVKEHKLVTVCEEAGCPNIGECWAK
KHATFMIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPK
TTIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRN
EVLQLMDDLRTADVDFLTIGQYLQPTRKHHAVEAFITPDEFAAYAEVANAKGFLLVSASPLTRSSHHAGEDFARLKAARL
ERLGR

Sequences:

>Translated_325_residues
MPPLADASTETLSPAEQAAVRHPEKAHRPDQPIARKPDWIRVKAPGSPEWVETQKIVKEHKLVTVCEEAGCPNIGECWAK
KHATFMIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPK
TTIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRN
EVLQLMDDLRTADVDFLTIGQYLQPTRKHHAVEAFITPDEFAAYAEVANAKGFLLVSASPLTRSSHHAGEDFARLKAARL
ERLGR
>Mature_324_residues
PPLADASTETLSPAEQAAVRHPEKAHRPDQPIARKPDWIRVKAPGSPEWVETQKIVKEHKLVTVCEEAGCPNIGECWAKK
HATFMIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKT
TIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRNE
VLQLMDDLRTADVDFLTIGQYLQPTRKHHAVEAFITPDEFAAYAEVANAKGFLLVSASPLTRSSHHAGEDFARLKAARLE
RLGR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=46.6898954703833, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=47.1544715447154, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=51.0714285714286, Blast_Score=287, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=42.7083333333333, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=43.5810810810811, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=44.2105263157895, Blast_Score=250, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BEII9 (B2IB58)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832631.1
- GeneID:   6201039
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1509
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35805; Mature: 35674

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLADASTETLSPAEQAAVRHPEKAHRPDQPIARKPDWIRVKAPGSPEWVETQKIVKEH
CCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
KLVTVCEEAGCPNIGECWAKKHATFMIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADA
HHHEEHHHCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKTTIEVLTPDFLRKDGALEIV
HHHCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEE
VAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRN
EEECCCHHCCCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHH
EVLQLMDDLRTADVDFLTIGQYLQPTRKHHAVEAFITPDEFAAYAEVANAKGFLLVSASP
HHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCEEEEECHHHHHHHHHHHCCCEEEEEECCC
LTRSSHHAGEDFARLKAARLERLGR
CCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPLADASTETLSPAEQAAVRHPEKAHRPDQPIARKPDWIRVKAPGSPEWVETQKIVKEH
CCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
KLVTVCEEAGCPNIGECWAKKHATFMIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADA
HHHEEHHHCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKTTIEVLTPDFLRKDGALEIV
HHHCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEE
VAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRN
EEECCCHHCCCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHH
EVLQLMDDLRTADVDFLTIGQYLQPTRKHHAVEAFITPDEFAAYAEVANAKGFLLVSASP
HHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCEEEEECHHHHHHHHHHHCCCEEEEEECCC
LTRSSHHAGEDFARLKAARLERLGR
CCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA