| Definition | Beijerinckia indica subsp. indica ATCC 9039 chromosome, complete genome. |
|---|---|
| Accession | NC_010581 |
| Length | 4,170,153 |
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The map label for this gene is hdhA [C]
Identifier: 182678437
GI number: 182678437
Start: 1664272
End: 1665018
Strand: Direct
Name: hdhA [C]
Synonym: Bind_1456
Alternate gene names: 182678437
Gene position: 1664272-1665018 (Clockwise)
Preceding gene: 182678436
Following gene: 182678439
Centisome position: 39.91
GC content: 59.57
Gene sequence:
>747_bases ATGACTCAACTGCATGGCAAGAAAGCCTTGGTCACGGGCGCTTCCCGTGGCATGGGCCGCGCGACGGCACTCGCTCTGGC AGCTGCCGGCGCGGAGGTGCTGGTCCACTACAGTTTCAATGAGAAGGCAGCAAACACCGTCGTCGAGGAGATCATAGGTA ACGGCGGAAAAGCCCATGCCGTTGCAGCTGACTTGCGCGACATTCAAGGGACCTTCGACCTATCCGAGCGCGTCCGTGAG ATTACTTCCGGAAAACTCGACATTCTCGTCGCCAATGCGGGTATCGCACGTCCAGCCCGTCTCGAAGATACGACAGTTGC AGACTTCGACGACATGTTTGCCGTCAATGTCAAAGCCCCTTTCTTTCTGGTGCAACAGTTGTCGCCTATCATGGGCGCTG GCAGCAGCATTATTCTCACCTCGTCGTTGGCGACCCGCACGACCGTCGGCGACCTCTCAGCCTATGCCGCCTCGAAGGGA GCGATCTCGACATTGGTGACCCATTTCGCAGCAGAGCTGGGTTCGCGTGGTATCCGCGTCAACGCGATCGCACCGGGTGT CGTCGCCACCGACATGTCGAGTTTCGTCGAAACGACCGAGGGTCAGGAATTCACCCTGGGTATCCAGGCGATCAAGCGCG TGGCGACGCCGGAGGATATTGCCGGGGTGATCGTGTTCCTTGCAGGTGATGATGCCCGCTGGGTTACGGGGGACGTCATT CGGGCCGATGGCGGTTCCAAGCTGTGA
Upstream 100 bases:
>100_bases AAGCGAAGACCACCCGGTCCTTCTACACGATGATGGACGGCAGCTTCTACGTCATGTCCGGCACGATCTCAGAGTAAGAA ACAACGCAAGGAAGAACACC
Downstream 100 bases:
>100_bases CGTCGGCGATGGATTCGCTGAGTATAATGGGCCGCATCGATGTTGACGCCGTGAGCGCTTAGGGGATGCGCCACCTGATT GCAATAAGGGCAGGGATCGT
Product: short-chain dehydrogenase/reductase SDR
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: ORFC [H]
Number of amino acids: Translated: 248; Mature: 247
Protein sequence:
>248_residues MTQLHGKKALVTGASRGMGRATALALAAAGAEVLVHYSFNEKAANTVVEEIIGNGGKAHAVAADLRDIQGTFDLSERVRE ITSGKLDILVANAGIARPARLEDTTVADFDDMFAVNVKAPFFLVQQLSPIMGAGSSIILTSSLATRTTVGDLSAYAASKG AISTLVTHFAAELGSRGIRVNAIAPGVVATDMSSFVETTEGQEFTLGIQAIKRVATPEDIAGVIVFLAGDDARWVTGDVI RADGGSKL
Sequences:
>Translated_248_residues MTQLHGKKALVTGASRGMGRATALALAAAGAEVLVHYSFNEKAANTVVEEIIGNGGKAHAVAADLRDIQGTFDLSERVRE ITSGKLDILVANAGIARPARLEDTTVADFDDMFAVNVKAPFFLVQQLSPIMGAGSSIILTSSLATRTTVGDLSAYAASKG AISTLVTHFAAELGSRGIRVNAIAPGVVATDMSSFVETTEGQEFTLGIQAIKRVATPEDIAGVIVFLAGDDARWVTGDVI RADGGSKL >Mature_247_residues TQLHGKKALVTGASRGMGRATALALAAAGAEVLVHYSFNEKAANTVVEEIIGNGGKAHAVAADLRDIQGTFDLSERVREI TSGKLDILVANAGIARPARLEDTTVADFDDMFAVNVKAPFFLVQQLSPIMGAGSSIILTSSLATRTTVGDLSAYAASKGA ISTLVTHFAAELGSRGIRVNAIAPGVVATDMSSFVETTEGQEFTLGIQAIKRVATPEDIAGVIVFLAGDDARWVTGDVIR ADGGSKL
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=33.0708661417323, Blast_Score=105, Evalue=3e-23, Organism=Homo sapiens, GI15277342, Length=254, Percent_Identity=32.6771653543307, Blast_Score=102, Evalue=2e-22, Organism=Homo sapiens, GI5031737, Length=248, Percent_Identity=31.8548387096774, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=33.6, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI66933014, Length=258, Percent_Identity=29.8449612403101, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI33667109, Length=199, Percent_Identity=32.1608040201005, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI126723750, Length=256, Percent_Identity=30.46875, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=29.8387096774194, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI19923817, Length=257, Percent_Identity=25.6809338521401, Blast_Score=75, Evalue=7e-14, Organism=Homo sapiens, GI31542939, Length=240, Percent_Identity=32.5, Blast_Score=74, Evalue=9e-14, Organism=Homo sapiens, GI109715829, Length=256, Percent_Identity=28.515625, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI20149619, Length=245, Percent_Identity=30.2040816326531, Blast_Score=68, Evalue=6e-12, Organism=Homo sapiens, GI142976729, Length=192, Percent_Identity=30.2083333333333, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI209862901, Length=201, Percent_Identity=29.3532338308458, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI19923983, Length=201, Percent_Identity=29.3532338308458, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI225579078, Length=257, Percent_Identity=29.9610894941634, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI4503301, Length=204, Percent_Identity=27.9411764705882, Blast_Score=65, Evalue=4e-11, Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=39.5918367346939, Blast_Score=127, Evalue=5e-31, Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=36.4, Blast_Score=119, Evalue=1e-28, Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=37.5, Blast_Score=115, Evalue=2e-27, Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=32.6530612244898, Blast_Score=111, Evalue=3e-26, Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=34.3629343629344, Blast_Score=110, Evalue=1e-25, Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=34.2741935483871, Blast_Score=107, Evalue=8e-25, Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=30.327868852459, Blast_Score=105, Evalue=2e-24, Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-24, Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=32.6530612244898, Blast_Score=101, Evalue=4e-23, Organism=Escherichia coli, GI87082160, Length=254, Percent_Identity=33.4645669291339, Blast_Score=100, Evalue=8e-23, Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=29.9595141700405, Blast_Score=94, Evalue=8e-21, Organism=Escherichia coli, GI1787526, Length=254, Percent_Identity=31.8897637795276, Blast_Score=81, Evalue=6e-17, Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=28.515625, Blast_Score=75, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=32.2709163346614, Blast_Score=107, Evalue=7e-24, Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=33.0769230769231, Blast_Score=106, Evalue=9e-24, Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=32.9317269076305, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=31.203007518797, Blast_Score=97, Evalue=6e-21, Organism=Caenorhabditis elegans, GI193204405, Length=266, Percent_Identity=34.2105263157895, Blast_Score=94, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=34.0823970037453, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17563726, Length=269, Percent_Identity=31.2267657992565, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=31.9702602230483, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=29.8387096774194, Blast_Score=90, Evalue=9e-19, Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=30.6818181818182, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=29.9242424242424, Blast_Score=88, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17508895, Length=194, Percent_Identity=32.9896907216495, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=33.4586466165414, Blast_Score=87, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=32.3308270676692, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=28.968253968254, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI17562904, Length=279, Percent_Identity=25.4480286738351, Blast_Score=84, Evalue=7e-17, Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=28.125, Blast_Score=84, Evalue=8e-17, Organism=Caenorhabditis elegans, GI17560220, Length=278, Percent_Identity=33.0935251798561, Blast_Score=84, Evalue=8e-17, Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17560332, Length=274, Percent_Identity=31.7518248175182, Blast_Score=81, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=30.8270676691729, Blast_Score=81, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17564282, Length=195, Percent_Identity=28.7179487179487, Blast_Score=76, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17544670, Length=280, Percent_Identity=28.2142857142857, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=29.2134831460674, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI71997099, Length=209, Percent_Identity=29.6650717703349, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17568967, Length=245, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=9e-13, Organism=Caenorhabditis elegans, GI71982365, Length=191, Percent_Identity=30.3664921465969, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17565030, Length=258, Percent_Identity=29.0697674418605, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17508651, Length=252, Percent_Identity=27.3809523809524, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17536025, Length=257, Percent_Identity=26.4591439688716, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17538182, Length=254, Percent_Identity=28.740157480315, Blast_Score=68, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17555612, Length=209, Percent_Identity=30.1435406698565, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI193203117, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=3e-11, Organism=Caenorhabditis elegans, GI193203115, Length=194, Percent_Identity=29.8969072164948, Blast_Score=65, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=24.7058823529412, Blast_Score=64, Evalue=8e-11, Organism=Saccharomyces cerevisiae, GI6322227, Length=206, Percent_Identity=29.126213592233, Blast_Score=69, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6323882, Length=256, Percent_Identity=30.078125, Blast_Score=66, Evalue=4e-12, Organism=Saccharomyces cerevisiae, GI6322861, Length=186, Percent_Identity=31.7204301075269, Blast_Score=65, Evalue=1e-11, Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=34.8, Blast_Score=112, Evalue=3e-25, Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=35.4330708661417, Blast_Score=110, Evalue=6e-25, Organism=Drosophila melanogaster, GI21357041, Length=259, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=7e-23, Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=3e-22, Organism=Drosophila melanogaster, GI28571526, Length=260, Percent_Identity=29.6153846153846, Blast_Score=100, Evalue=2e-21, Organism=Drosophila melanogaster, GI24644337, Length=217, Percent_Identity=30.8755760368664, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=30.8, Blast_Score=90, Evalue=1e-18, Organism=Drosophila melanogaster, GI24640492, Length=200, Percent_Identity=28.5, Blast_Score=84, Evalue=8e-17, Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=29.3650793650794, Blast_Score=81, Evalue=8e-16, Organism=Drosophila melanogaster, GI21357673, Length=199, Percent_Identity=29.6482412060301, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI17737361, Length=257, Percent_Identity=26.4591439688716, Blast_Score=65, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 25687; Mature: 25555
Theoretical pI: Translated: 5.32; Mature: 5.32
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTQLHGKKALVTGASRGMGRATALALAAAGAEVLVHYSFNEKAANTVVEEIIGNGGKAHA CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHH VAADLRDIQGTFDLSERVREITSGKLDILVANAGIARPARLEDTTVADFDDMFAVNVKAP HHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEECCCCCEEEEECCCH FFLVQQLSPIMGAGSSIILTSSLATRTTVGDLSAYAASKGAISTLVTHFAAELGSRGIRV HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE NAIAPGVVATDMSSFVETTEGQEFTLGIQAIKRVATPEDIAGVIVFLAGDDARWVTGDVI EEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCEE RADGGSKL ECCCCCCC >Mature Secondary Structure TQLHGKKALVTGASRGMGRATALALAAAGAEVLVHYSFNEKAANTVVEEIIGNGGKAHA CCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHH VAADLRDIQGTFDLSERVREITSGKLDILVANAGIARPARLEDTTVADFDDMFAVNVKAP HHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEECCCCCEEEEECCCH FFLVQQLSPIMGAGSSIILTSSLATRTTVGDLSAYAASKGAISTLVTHFAAELGSRGIRV HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE NAIAPGVVATDMSSFVETTEGQEFTLGIQAIKRVATPEDIAGVIVFLAGDDARWVTGDVI EEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCEE RADGGSKL ECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8868443 [H]