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The map label for this gene is pyrG

Identifier: 182678434

GI number: 182678434

Start: 1660860

End: 1662488

Strand: Direct

Name: pyrG

Synonym: Bind_1452

Alternate gene names: 182678434

Gene position: 1660860-1662488 (Clockwise)

Preceding gene: 182678433

Following gene: 182678436

Centisome position: 39.83

GC content: 60.04

Gene sequence:

>1629_bases
ATGGCGCGGTACATTTTCATCACCGGCGGCGTGGTTTCCTCCCTTGGCAAAGGTCTGGCCTCGGCGGCGCTCGGCGCTCT
TCTCCAGGCGCGCGGCTATACCGTGCGGCTGCGCAAGCTCGACCCTTATCTGAACATCGATCCGGGGACGATGAGTCCTT
ATCAGCATGGGGAGGTCTTCGTCACCGATGACGGTGCCGAGACGGATCTCGACCTTGGCCATTACGAGCGTTTCACTGGC
CGCCCGGCCGTCCGCGACGACAATATCACCACCGGCCGCATCTATCGGGATATTATCGCCAAGGAACGGCGCGGCGATTA
TCTCGGCGCGACGGTCCAGGTCGTGCCGCATGTGACCAATGCCATCAAGGAATTCATCCTGACCGGCAACGAGGGGCTTG
ATTTCGTCCTGGTCGAGATTGGGGGCACTGTCGGCGATATCGAGGGCCTGCCGTTCTTCGAGGCGATTCGTCAGCTCGGC
AATGAGCTGCCGCGCGGCCATGCCATTTATATCCATTTGACACTGCTCCCCTACATCCCGAGCGCCGGGGAGCTCAAAAC
CAAGCCGACGCAGCATTCGGTCAAGGAATTGCGGTCGATCGGCATTCAGCCGCATATTCTGCTCTGCCGCACCGATCGTC
CGATTCCCTTTGAGGAGCGCCGCAAGCTCGGGCTTTTCTGCAATGTGCGGGAAAATGCGGTGATCGAGGCGCGCGACGTC
TCCTCGATCTATGACGTGCCGCATGCCTATCATGCGGCGGGGCTCGATCAGGAAGTGCTTTCGGCCTTTGGCATAGAGCC
CGCGCCCAAGCCGGATATGAGCCGCTGGAAGGCGGTGATGGAGCGGATCGACAATCCCGAAGGCGAGGTGACGATCGCCA
TCGTCGGCAAATATACGGGGTTGAAGGATGCTTATAAGTCGCTGATCGAGGCCTTGTCCCACGGTGGTATCGCCAATCGG
GTACGGGTCAATCTGGATTGGATCGAGTCGGAAATTTTCGAGGGGACCGATCCGGCGCCGCATCTCGAACATGTGCATGG
CATTCTGGTGCCGGGCGGTTTTGGCCAGCGCGGCGCCGAGGGAAAGATGCTCGCCGCGCGTTTTGCCCGCCAGAGGAAAG
TCCCCTTTTTCGGTATTTGTTTCGGCATGCAGATGGCAGTGATCGAGGCCGTGCGCTCGCTCGCCGATATTCCGCTCGCC
AATTCGACTGAATTCGGCCCGACCAATGAACCCGTCGTCGGCCTGATGACGGAATGGATGCGCGACAACGAGTTGCAGAT
GCGCGCGGCGGAAGGTGATCTTGGCGGCACGATGCGGCTCGGAGCCTATCCGGCCTTGCTCGCGGCCGGTTCCAAGATTG
CCGAAATCTATGGCGCTACGGAAATTTCCGAGCGTCATCGCCATCGCTACGAGGTCAATACGGCCTATCGTGAACAATTG
GCCGAAAAGGGCATGATCTTCTCCGGCCTGTCTCCAGACGGCTTGCTGCCGGAAGTGGTGGAATTTCAGGATCACCCCTG
GTTCATCGGGGTGCAATATCACCCCGAGCTGAAATCACGGCCTTTCGCGCCGCATCCCCTCTTTTCCAGTTTCATCGCGG
CGGCCATGGAGCAGAGCCGGCTGGTTTAG

Upstream 100 bases:

>100_bases
GCCAGCATTTGCGCGGGCCTTGAGCCTTGTTTGAAAAAGCGGGGACAGTCTTTCGAAAAGACTCCCGCTTCGGCTTCGAA
AAGGATAAGGCTGTCAAACC

Downstream 100 bases:

>100_bases
TGCCGATTGGATATTCCGATCTGCTCCTGTTGATTGCCAGCCGAACCTTTCTGATTGCGACAGGTCGTAATCAGAAAGAC
GGGGGATTAGATGCGGCAGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPAVRDDNITTGRIYRDIIAKERRGDYLGATVQVVPHVTNAIKEFILTGNEGLDFVLVEIGGTVGDIEGLPFFEAIRQLG
NELPRGHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPHILLCRTDRPIPFEERRKLGLFCNVRENAVIEARDV
SSIYDVPHAYHAAGLDQEVLSAFGIEPAPKPDMSRWKAVMERIDNPEGEVTIAIVGKYTGLKDAYKSLIEALSHGGIANR
VRVNLDWIESEIFEGTDPAPHLEHVHGILVPGGFGQRGAEGKMLAARFARQRKVPFFGICFGMQMAVIEAVRSLADIPLA
NSTEFGPTNEPVVGLMTEWMRDNELQMRAAEGDLGGTMRLGAYPALLAAGSKIAEIYGATEISERHRHRYEVNTAYREQL
AEKGMIFSGLSPDGLLPEVVEFQDHPWFIGVQYHPELKSRPFAPHPLFSSFIAAAMEQSRLV

Sequences:

>Translated_542_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPAVRDDNITTGRIYRDIIAKERRGDYLGATVQVVPHVTNAIKEFILTGNEGLDFVLVEIGGTVGDIEGLPFFEAIRQLG
NELPRGHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPHILLCRTDRPIPFEERRKLGLFCNVRENAVIEARDV
SSIYDVPHAYHAAGLDQEVLSAFGIEPAPKPDMSRWKAVMERIDNPEGEVTIAIVGKYTGLKDAYKSLIEALSHGGIANR
VRVNLDWIESEIFEGTDPAPHLEHVHGILVPGGFGQRGAEGKMLAARFARQRKVPFFGICFGMQMAVIEAVRSLADIPLA
NSTEFGPTNEPVVGLMTEWMRDNELQMRAAEGDLGGTMRLGAYPALLAAGSKIAEIYGATEISERHRHRYEVNTAYREQL
AEKGMIFSGLSPDGLLPEVVEFQDHPWFIGVQYHPELKSRPFAPHPLFSSFIAAAMEQSRLV
>Mature_541_residues
ARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGR
PAVRDDNITTGRIYRDIIAKERRGDYLGATVQVVPHVTNAIKEFILTGNEGLDFVLVEIGGTVGDIEGLPFFEAIRQLGN
ELPRGHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPHILLCRTDRPIPFEERRKLGLFCNVRENAVIEARDVS
SIYDVPHAYHAAGLDQEVLSAFGIEPAPKPDMSRWKAVMERIDNPEGEVTIAIVGKYTGLKDAYKSLIEALSHGGIANRV
RVNLDWIESEIFEGTDPAPHLEHVHGILVPGGFGQRGAEGKMLAARFARQRKVPFFGICFGMQMAVIEAVRSLADIPLAN
STEFGPTNEPVVGLMTEWMRDNELQMRAAEGDLGGTMRLGAYPALLAAGSKIAEIYGATEISERHRHRYEVNTAYREQLA
EKGMIFSGLSPDGLLPEVVEFQDHPWFIGVQYHPELKSRPFAPHPLFSSFIAAAMEQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.165170556553, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=42.9338103756708, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=42.9338103756708, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=42.9338103756708, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=56.8773234200744, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25148299, Length=615, Percent_Identity=36.5853658536585, Blast_Score=393, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319432, Length=568, Percent_Identity=41.1971830985916, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40.5263157894737, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=562, Percent_Identity=43.0604982206406, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=42.2924901185771, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_BEII9 (B2IKQ2)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832580.1
- ProteinModelPortal:   B2IKQ2
- GeneID:   6200399
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1452
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59844; Mature: 59713

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 514-514 ACT_SITE 516-516

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNIDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPAVRDDNITTGRIYRDIIAKERRGDYLGATVQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEFILTGNEGLDFVLVEIGGTVGDIEGLPFFEAIRQLGNELPRGHAIYIHLTLLPYIP
HHHHHHCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPHILLCRTDRPIPFEERRKLGLFCNVRENAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCEEEECCCCCEEEECCH
SSIYDVPHAYHAAGLDQEVLSAFGIEPAPKPDMSRWKAVMERIDNPEGEVTIAIVGKYTG
HHHHCCCHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALSHGGIANRVRVNLDWIESEIFEGTDPAPHLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHCCEEEECCCCCCCCCC
GKMLAARFARQRKVPFFGICFGMQMAVIEAVRSLADIPLANSTEFGPTNEPVVGLMTEWM
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RDNELQMRAAEGDLGGTMRLGAYPALLAAGSKIAEIYGATEISERHRHRYEVNTAYREQL
CCCCEEEEECCCCCCCEEECCCCHHHHHCCCHHHHHHCCHHHHHHHHHHEECCHHHHHHH
AEKGMIFSGLSPDGLLPEVVEFQDHPWFIGVQYHPELKSRPFAPHPLFSSFIAAAMEQSR
HHCCCEEECCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNIDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPAVRDDNITTGRIYRDIIAKERRGDYLGATVQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEFILTGNEGLDFVLVEIGGTVGDIEGLPFFEAIRQLGNELPRGHAIYIHLTLLPYIP
HHHHHHCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPHILLCRTDRPIPFEERRKLGLFCNVRENAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCEEEECCCCCEEEECCH
SSIYDVPHAYHAAGLDQEVLSAFGIEPAPKPDMSRWKAVMERIDNPEGEVTIAIVGKYTG
HHHHCCCHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALSHGGIANRVRVNLDWIESEIFEGTDPAPHLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHCCEEEECCCCCCCCCC
GKMLAARFARQRKVPFFGICFGMQMAVIEAVRSLADIPLANSTEFGPTNEPVVGLMTEWM
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RDNELQMRAAEGDLGGTMRLGAYPALLAAGSKIAEIYGATEISERHRHRYEVNTAYREQL
CCCCEEEEECCCCCCCEEECCCCHHHHHCCCHHHHHHCCHHHHHHHHHHEECCHHHHHHH
AEKGMIFSGLSPDGLLPEVVEFQDHPWFIGVQYHPELKSRPFAPHPLFSSFIAAAMEQSR
HHCCCEEECCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA