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The map label for this gene is nolF [H]

Identifier: 182678375

GI number: 182678375

Start: 1597231

End: 1598340

Strand: Direct

Name: nolF [H]

Synonym: Bind_1392

Alternate gene names: 182678375

Gene position: 1597231-1598340 (Clockwise)

Preceding gene: 182678374

Following gene: 182678376

Centisome position: 38.3

GC content: 57.84

Gene sequence:

>1110_bases
ATGCACATTCATTCTGTGATAACGAGGCTCCACCTCTCTCGTTATACCGCTTTGTCCTTGATGCTGGTGTCATTGCCGCT
CGCTGCCTGTCGGGAGGAAACCCAGACGGTGGCGGCCGCCCCACGTCCCGTTCGCACGATGACGATCGAGAAAAGCGAAT
TGGGCGAGGAAATTTCCCTGACGGGACATCTTCAAGCGGAAAACGAGGCGACTCTCGCCTTTCGTATCGGCGGCCGAATC
ATCGAAAGATCAGTCAATGTCGGCGACCATGTCACTGTCGATCAAGTTATGGCGAAACTCGATTCGCAGAATGAACTGAA
TAGCATGCGCTCGGCGCAAGCCAATTTGACCGCGGCTCAGGGCAGATTGAAGGAAGCCGGCAATAATTTCAGCCGTCAGC
AATCTTTGCTCGAAAGAGGTTTTACAACCAGGGTCCTGTTTGATCAGGCACAACAGGTTTACAATACGGCTTTGTCTCAG
GTCGATGATGCGCGGGCGCAATTGAAGATCGCCGAGGATCGGCTCAGCTATACGGAACTCAAGGCCGATGCGGCAGGGGC
CATTATCGCGCGCGGCGCGGAGGCCGGCGAGGTGGTGCAGGCCGGGCAAATGATCTTCAAGCTCGCGCGGCAAGGTGGCT
GGGATGCTGTCTTCAATGTCCCTGCTCAAACCTTACGCGCGGCGCCGTCTGATCCCAAAATCATTGTGTCCCTGACGGAT
GATCCAACAGTGACGGCAACGGCGCGTGTCCGGCAAGTTGATCCGCAAGCCGATCCCGTGACCAGGACCTTTCGAGTGAA
GGTCAGCGTGGATGATCCACCCCCCGCCATGCGGCTTGGTGCTACCGTCGTCGGGCGTATGCAATTGGAATCCTCGCCGG
GTATAGCGATCCCTGCGGCCGCTCTCACTGAAATTGATCGCCAACCGAGCGTGTGGATCGTTGATCCTGCGACCGAGACC
GTCTCCATGCGCCGTATTGGCGTGGTCCGTTTCGACCCAGGGACTGTCGTCGTGAGTGATGGATTGGCGCCGGGCGACAT
TGTCGTGACGGCCGGTGTCCAGGCCTTGCATCCGGGGCAAAAAGTTCGTTTGCTCGGGTCGTCGACATGA

Upstream 100 bases:

>100_bases
AAAGTCCGATGCTCTAGCCCAACGCCTTTGGTGTCATTGCAAGCGGTTCAGCTACAGGTATAGTAAATAACGCTTTTCGA
CTCGGGAGCCTATGGAAAGA

Downstream 100 bases:

>100_bases
GCGGGCTCAATCTTTCAGCTTGGGCGCTGAAACATCGTTCCTTCACGATCTATTGCATGATCGCGGTGACGATTGCCGGG
CTTGCGTCCTATTTCCAGCT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MHIHSVITRLHLSRYTALSLMLVSLPLAACREETQTVAAAPRPVRTMTIEKSELGEEISLTGHLQAENEATLAFRIGGRI
IERSVNVGDHVTVDQVMAKLDSQNELNSMRSAQANLTAAQGRLKEAGNNFSRQQSLLERGFTTRVLFDQAQQVYNTALSQ
VDDARAQLKIAEDRLSYTELKADAAGAIIARGAEAGEVVQAGQMIFKLARQGGWDAVFNVPAQTLRAAPSDPKIIVSLTD
DPTVTATARVRQVDPQADPVTRTFRVKVSVDDPPPAMRLGATVVGRMQLESSPGIAIPAAALTEIDRQPSVWIVDPATET
VSMRRIGVVRFDPGTVVVSDGLAPGDIVVTAGVQALHPGQKVRLLGSST

Sequences:

>Translated_369_residues
MHIHSVITRLHLSRYTALSLMLVSLPLAACREETQTVAAAPRPVRTMTIEKSELGEEISLTGHLQAENEATLAFRIGGRI
IERSVNVGDHVTVDQVMAKLDSQNELNSMRSAQANLTAAQGRLKEAGNNFSRQQSLLERGFTTRVLFDQAQQVYNTALSQ
VDDARAQLKIAEDRLSYTELKADAAGAIIARGAEAGEVVQAGQMIFKLARQGGWDAVFNVPAQTLRAAPSDPKIIVSLTD
DPTVTATARVRQVDPQADPVTRTFRVKVSVDDPPPAMRLGATVVGRMQLESSPGIAIPAAALTEIDRQPSVWIVDPATET
VSMRRIGVVRFDPGTVVVSDGLAPGDIVVTAGVQALHPGQKVRLLGSST
>Mature_369_residues
MHIHSVITRLHLSRYTALSLMLVSLPLAACREETQTVAAAPRPVRTMTIEKSELGEEISLTGHLQAENEATLAFRIGGRI
IERSVNVGDHVTVDQVMAKLDSQNELNSMRSAQANLTAAQGRLKEAGNNFSRQQSLLERGFTTRVLFDQAQQVYNTALSQ
VDDARAQLKIAEDRLSYTELKADAAGAIIARGAEAGEVVQAGQMIFKLARQGGWDAVFNVPAQTLRAAPSDPKIIVSLTD
DPTVTATARVRQVDPQADPVTRTFRVKVSVDDPPPAMRLGATVVGRMQLESSPGIAIPAAALTEIDRQPSVWIVDPATET
VSMRRIGVVRFDPGTVVVSDGLAPGDIVVTAGVQALHPGQKVRLLGSST

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=380, Percent_Identity=23.4210526315789, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082036, Length=332, Percent_Identity=22.5903614457831, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39635; Mature: 39635

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHSVITRLHLSRYTALSLMLVSLPLAACREETQTVAAAPRPVRTMTIEKSELGEEISL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCEEEE
TGHLQAENEATLAFRIGGRIIERSVNVGDHVTVDQVMAKLDSQNELNSMRSAQANLTAAQ
EEEEECCCCEEEEEEECCEEEECCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCHHHHH
GRLKEAGNNFSRQQSLLERGFTTRVLFDQAQQVYNTALSQVDDARAQLKIAEDRLSYTEL
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHH
KADAAGAIIARGAEAGEVVQAGQMIFKLARQGGWDAVFNVPAQTLRAAPSDPKIIVSLTD
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEEECC
DPTVTATARVRQVDPQADPVTRTFRVKVSVDDPPPAMRLGATVVGRMQLESSPGIAIPAA
CCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCHHHHCHHEEEEEEECCCCCEEECHH
ALTEIDRQPSVWIVDPATETVSMRRIGVVRFDPGTVVVSDGLAPGDIVVTAGVQALHPGQ
HHHHCCCCCCEEEECCCCHHEEEHEEEEEEECCCEEEEECCCCCCCEEEECCCHHCCCCC
KVRLLGSST
EEEEEECCC
>Mature Secondary Structure
MHIHSVITRLHLSRYTALSLMLVSLPLAACREETQTVAAAPRPVRTMTIEKSELGEEISL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCEEEE
TGHLQAENEATLAFRIGGRIIERSVNVGDHVTVDQVMAKLDSQNELNSMRSAQANLTAAQ
EEEEECCCCEEEEEEECCEEEECCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCHHHHH
GRLKEAGNNFSRQQSLLERGFTTRVLFDQAQQVYNTALSQVDDARAQLKIAEDRLSYTEL
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHH
KADAAGAIIARGAEAGEVVQAGQMIFKLARQGGWDAVFNVPAQTLRAAPSDPKIIVSLTD
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEEECC
DPTVTATARVRQVDPQADPVTRTFRVKVSVDDPPPAMRLGATVVGRMQLESSPGIAIPAA
CCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCHHHHCHHEEEEEEECCCCCEEECHH
ALTEIDRQPSVWIVDPATETVSMRRIGVVRFDPGTVVVSDGLAPGDIVVTAGVQALHPGQ
HHHHCCCCCCEEEECCCCHHEEEHEEEEEEECCCEEEEECCCCCCCEEEECCCHHCCCCC
KVRLLGSST
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]