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The map label for this gene is tuf [H]

Identifier: 182678335

GI number: 182678335

Start: 1567399

End: 1568589

Strand: Direct

Name: tuf [H]

Synonym: Bind_1352

Alternate gene names: 182678335

Gene position: 1567399-1568589 (Clockwise)

Preceding gene: 182678334

Following gene: 182678336

Centisome position: 37.59

GC content: 60.45

Gene sequence:

>1191_bases
ATGGGCAAGGAAAAATTTGAGCGGACGAAGCCGCATTGCAACATTGGAACGATTGGCCATGTTGACCATGGCAAGACGTC
ACTGACGGCGGCGATCACCAAGGTTCTGGCGGAAGCGGGCGGCGCGACCTTTACGGCCTATGATCAGATCGACAAGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATCTCGACGGCGCATGTGGAATATGAGACGCCGAAGCGGCATTACGCGCATGTG
GACTGCCCCGGCCACGCCGACTATGTGAAAAACATGATCACCGGCGCGGCGCAGATGGACGGGGCGATCCTGGTGGTTTC
GGCGGCCGATGGCCCGATGCCGCAGACGCGCGAGCATATTCTTCTGGCGCGCCAGGTCGGTGTTCCGGCGCTGGTCGTGT
TCCTGAACAAGGTCGACATGGTCGATGACGCGGAACTGCTCGAACTCGTCGAGCTGGAAGTGCGCGAGCTTCTGTCGAAA
TATGACTTCCCCGGCGATGACATTCCGATCACCAAGGGTTCGGCCCTGTGCGCCTTGGAAAACCGCAATCCGGAAATCGG
TCATGATGCGGTTCTCGCTCTGATGCAGACGGTGGATGATTATATTCCGCAGCCGGAACGTCCGATCGATCTGCCGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCAGGCCGCGGTACGGTTGTGACAGGCCGCGTCGAGCGTGGCGTCATCAAG
GTCGGTGAGGAAATCGAGATCGTCGGTCTGCGGCCGACGGTGAAGACGACGGTGACCGGCGTCGAAATGTTCCGCAAGCT
TCTGGACCAGGGTCAGGCGGGCGACAATATCGGCGCTCTGCTGCGTGGCACCAAGCGCGAGGATGTGGAGCGTGGCCAGA
TCCTGTGCAAGCCGGGTTCCGTGAAGCCGCACACCAAGTTCAAGGCGGAAGCCTATATTCTGACCAAGGATGAGGGTGGC
CGGCATACGCCGTTCTTCACCAACTATCGTCCGCAGTTCTATTTCCGGACGACCGATGTGACGGGCGTCGTGACGCTGCC
GGAAGGCACGGAAATGGTGATGCCTGGCGATAATGTGACGATGGATGTGGAGCTGATCGTTCCGATCGCGATGGAAGAGA
AGCTGCGCTTCGCCATCCGCGAAGGTGGCCGCACGGTCGGCGCCGGCGTCGTCGCCTCCATTACCGAGTAA

Upstream 100 bases:

>100_bases
ATTATGAGCAGGTCCCCGCGAATGAAGCGGCGAAAGTTCAAGCGAAATACGCTTGATTGGTCGCACGGGGCTAGCAAAGA
GATCCTAGAGGAGCCGGACG

Downstream 100 bases:

>100_bases
AAGCTTGAAGCGGCCGGGCTCGGTCCGGCCGCTTGGTCTCTCCACTTCCGGCATTTGTTGGAAGTGGATAGGTCTCCGAT
CTGCTGCCTAGAGCAGTGGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGKEKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTAHVEYETPKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDAELLELVELEVRELLSK
YDFPGDDIPITKGSALCALENRNPEIGHDAVLALMQTVDDYIPQPERPIDLPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGLRPTVKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQILCKPGSVKPHTKFKAEAYILTKDEGG
RHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVELIVPIAMEEKLRFAIREGGRTVGAGVVASITE

Sequences:

>Translated_396_residues
MGKEKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTAHVEYETPKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDAELLELVELEVRELLSK
YDFPGDDIPITKGSALCALENRNPEIGHDAVLALMQTVDDYIPQPERPIDLPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGLRPTVKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQILCKPGSVKPHTKFKAEAYILTKDEGG
RHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVELIVPIAMEEKLRFAIREGGRTVGAGVVASITE
>Mature_395_residues
GKEKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTAHVEYETPKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDAELLELVELEVRELLSKY
DFPGDDIPITKGSALCALENRNPEIGHDAVLALMQTVDDYIPQPERPIDLPFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGLRPTVKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQILCKPGSVKPHTKFKAEAYILTKDEGGR
HTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVELIVPIAMEEKLRFAIREGGRTVGAGVVASITE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=57.3979591836735, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=31.5789473684211, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=31.5789473684211, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=30, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI4503475, Length=456, Percent_Identity=28.2894736842105, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=320, Percent_Identity=30, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194097354, Length=320, Percent_Identity=30, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018520, Length=320, Percent_Identity=30, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI46094014, Length=320, Percent_Identity=29.6875, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=27.9874213836478, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=26.9662921348315, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=306, Percent_Identity=26.797385620915, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=76.1421319796954, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=76.1421319796954, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=31.758530183727, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1789108, Length=266, Percent_Identity=30.0751879699248, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.8847117794486, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=42.8940568475452, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=30.3769401330377, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=30.3769401330377, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=28.604118993135, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.0182232346241, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=28.0182232346241, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.5486725663717, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.1879194630872, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=40.2597402597403, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=26.4957264957265, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=67.9292929292929, Blast_Score=551, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=30.5122494432071, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=30.5122494432071, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=27.0655270655271, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=27.9761904761905, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=26.8115942028986, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=24.6861924686192, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=60.613810741688, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=60.613810741688, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=458, Percent_Identity=29.2576419213974, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=458, Percent_Identity=29.2576419213974, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=458, Percent_Identity=29.2576419213974, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=458, Percent_Identity=29.2576419213974, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=28.8840262582057, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=28.8840262582057, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.6831683168317, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.2325581395349, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.3768545994065, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=289, Percent_Identity=26.9896193771626, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=289, Percent_Identity=26.9896193771626, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43228; Mature: 43097

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARG
CCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETPKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKVDMVDDAELLELVELEVRELLSKYDFPGDDIPITKGSALCALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEC
NRNPEIGHDAVLALMQTVDDYIPQPERPIDLPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGLRPTVKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQILCKPGS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHCCCCCEEEECCCC
VKPHTKFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVT
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEE
MDVELIVPIAMEEKLRFAIREGGRTVGAGVVASITE
EEEEEEEEEHHHHHHHHHHHHCCCEECCCEEEEECC
>Mature Secondary Structure 
GKEKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARG
CCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETPKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKVDMVDDAELLELVELEVRELLSKYDFPGDDIPITKGSALCALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEC
NRNPEIGHDAVLALMQTVDDYIPQPERPIDLPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGLRPTVKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQILCKPGS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHCCCCCEEEECCCC
VKPHTKFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVT
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEE
MDVELIVPIAMEEKLRFAIREGGRTVGAGVVASITE
EEEEEEEEEHHHHHHHHHHHHCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA