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The map label for this gene is lasT [C]

Identifier: 182678308

GI number: 182678308

Start: 1535462

End: 1536277

Strand: Direct

Name: lasT [C]

Synonym: Bind_1325

Alternate gene names: 182678308

Gene position: 1535462-1536277 (Clockwise)

Preceding gene: 182678305

Following gene: 182678309

Centisome position: 36.82

GC content: 63.73

Gene sequence:

>816_bases
ATGACCGGAGCCGGTACCGATCACAGTCGTGAGAATGTCGCTGGCGGCCCCGCCATCATCCTCGTGCGGCCACAGCTCGC
GGTCAATATCGGCATGTGCGCGCGAGCCATGGCGAATTTCGGCCTGTCCGACCTGCGTCTCGTTGCCCCGCGTGAAGGTT
GGCCGCGAGAAGGGCGTTTCAAGGACGAAGCGGAAGCCGCGGCGGCCGGCGCGGTGCATCTTCTGGCCTCGGCGAAACTT
TATGCCACCTTGCCGGAAGCGGTCGCCGATTTGAATTTTATCTGGGCGACCACGGCGCGCGAGCGCGGCCAGTGCAAGCG
CGTCGCGACACCTGCGCAGGCTATGCCCGAAAACGCGGCTCGGCTGGCACTGGGGGAGCGGCAGGGCATCTTGTTCGGGC
CTGAGCGCACGGGCCTTGCCAATGACGATATTGCGCTTGCCGACGCGATTCTCACCTTTCCGGTCAATCCCGCCTATGCC
TCGCTCAATCTGGCGCAGGCAGTCCTTTTGACCGGCTATGAATGGTTTCAGGCGGCCCATAATGCCGAGGCGCCGTTTCA
GCTGGCTGATTATTCGCCGCCGGCGCCCCGCGAGATGATCTTGTCCTTTTTCGACTATCTCGAAGGCGAACTCGACCGAT
CGGGCTTCTTCCGACCGGCCGGCAAGGCGCCCGTCATGCGGCGCAATTTGCGCAATATTTTCCACCGCATGCATTTGAGC
GAGCAGGATGTGCGGACCTTGCGCGGCATGGTCGTGCGGCTCGTCGAGGGTCCGCGCGCGCTCGTCACCCGTCGGGCCGA
CGATCATCAAGACTGA

Upstream 100 bases:

>100_bases
ATGCCAGGGCGTGAAAATTGGGTAGCGGGAACGCGATGATGATCCAAAGTGAGACAGGGGACGCTTTCCCCTGGTTGTGT
TGCGCTGGAGACCGGCCGCG

Downstream 100 bases:

>100_bases
CCGCGTTATACCAAAAATCAATAATTATCGCCTTTGATACGGGTGCCGTGCGTTGACTGCTCCGCAATGGAGATGTCGCC
GCCCGGATCAATTGGCTTTT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTGAGTDHSRENVAGGPAIILVRPQLAVNIGMCARAMANFGLSDLRLVAPREGWPREGRFKDEAEAAAAGAVHLLASAKL
YATLPEAVADLNFIWATTARERGQCKRVATPAQAMPENAARLALGERQGILFGPERTGLANDDIALADAILTFPVNPAYA
SLNLAQAVLLTGYEWFQAAHNAEAPFQLADYSPPAPREMILSFFDYLEGELDRSGFFRPAGKAPVMRRNLRNIFHRMHLS
EQDVRTLRGMVVRLVEGPRALVTRRADDHQD

Sequences:

>Translated_271_residues
MTGAGTDHSRENVAGGPAIILVRPQLAVNIGMCARAMANFGLSDLRLVAPREGWPREGRFKDEAEAAAAGAVHLLASAKL
YATLPEAVADLNFIWATTARERGQCKRVATPAQAMPENAARLALGERQGILFGPERTGLANDDIALADAILTFPVNPAYA
SLNLAQAVLLTGYEWFQAAHNAEAPFQLADYSPPAPREMILSFFDYLEGELDRSGFFRPAGKAPVMRRNLRNIFHRMHLS
EQDVRTLRGMVVRLVEGPRALVTRRADDHQD
>Mature_270_residues
TGAGTDHSRENVAGGPAIILVRPQLAVNIGMCARAMANFGLSDLRLVAPREGWPREGRFKDEAEAAAAGAVHLLASAKLY
ATLPEAVADLNFIWATTARERGQCKRVATPAQAMPENAARLALGERQGILFGPERTGLANDDIALADAILTFPVNPAYAS
LNLAQAVLLTGYEWFQAAHNAEAPFQLADYSPPAPREMILSFFDYLEGELDRSGFFRPAGKAPVMRRNLRNIFHRMHLSE
QDVRTLRGMVVRLVEGPRALVTRRADDHQD

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=37.1794871794872, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=31.5352697095436, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29612; Mature: 29481

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGTDHSRENVAGGPAIILVRPQLAVNIGMCARAMANFGLSDLRLVAPREGWPREGRF
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
KDEAEAAAAGAVHLLASAKLYATLPEAVADLNFIWATTARERGQCKRVATPAQAMPENAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCHHHCCHHHHCCCHHHCCCHHH
RLALGERQGILFGPERTGLANDDIALADAILTFPVNPAYASLNLAQAVLLTGYEWFQAAH
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NAEAPFQLADYSPPAPREMILSFFDYLEGELDRSGFFRPAGKAPVMRRNLRNIFHRMHLS
CCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
EQDVRTLRGMVVRLVEGPRALVTRRADDHQD
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
TGAGTDHSRENVAGGPAIILVRPQLAVNIGMCARAMANFGLSDLRLVAPREGWPREGRF
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
KDEAEAAAAGAVHLLASAKLYATLPEAVADLNFIWATTARERGQCKRVATPAQAMPENAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCHHHCCHHHHCCCHHHCCCHHH
RLALGERQGILFGPERTGLANDDIALADAILTFPVNPAYASLNLAQAVLLTGYEWFQAAH
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NAEAPFQLADYSPPAPREMILSFFDYLEGELDRSGFFRPAGKAPVMRRNLRNIFHRMHLS
CCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
EQDVRTLRGMVVRLVEGPRALVTRRADDHQD
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]