Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 182678271

GI number: 182678271

Start: 1495467

End: 1497059

Strand: Direct

Name: purH [H]

Synonym: Bind_1287

Alternate gene names: 182678271

Gene position: 1495467-1497059 (Clockwise)

Preceding gene: 182678270

Following gene: 182678272

Centisome position: 35.86

GC content: 59.95

Gene sequence:

>1593_bases
ATGGCTTCCGAGCTTCGCCGCGTCGAGCGGGCGCTGATTTCCGTTTCCGACAAGACGGGCCTCGTTGATTTCGCCCGTGC
CCTTGCCGCGCTTGGCATTGAACTGGTCTCGACCGGTGGCACCAGCAAAGCTCTGACCGAGGCGGGGCTGGCCGTTCGAG
ATGTTTCGGATCTCACCGGCTTTCCTGAATTGATGGATGGACGCCTCAAAACCCTGCATCCCAAGGTTCATGGCGGCCTT
CTGGCCATTCGCGAAAATCCCGAGCATGAGGCGGCCATGCTCGCGCATAATATCGCGCCGATCGATCTGTTGGTGGTTAA
TCTCTATCCGTTCGAGGCGACGATCGCCAAGGGCGCCCCCTTTGCCGATTGCATCGAAAATATTGATATTGGCGGTCCGG
CGATGATCCGGGCGGCCTCCAAGAATTTCAATGATGTCACGGTCGTCATCGAGCCTGAGGATTATGCCGCGATTCTAGCT
GAACTGCAGGGTCAAAATGGCGCGACCAGCCTCGAACTGCGCCGCAAACTCGCGCAAAAGGCTTTTGCCCGGACAGCGAC
TTATGACGCGACGATTTCCAACTGGTTCGCCAAGGAAATCGGTGAGACGACGCCGACTTATCGCGCTTTCGGCGGGAGGC
TGATCGAAGCCCTGCGCTATGGTGAAAACCCGCATCAGGCGGCAGCCTTTTACGCGCTGCCGCAGGGACGTGCCGGCGTC
ACCACGGCGCGGCAAGTTCAGGGCAAGGCGCTTTCTTATAATAACGTCAATGATACGGATGCGGCTTTCGAACTTGTGGC
CGAGTTCGATCCCAGCAAGCAGGCGGCGGTCGCCATTATCAAACATGCCAATCCCTGCGGCGTCGCCGAGGCTGCAAGCC
TAAAAGAGGCCTATTTGCTGGCGCTTCGTTGTGATCCGGTCTCGGCTTTCGGCGGCATCATTGCCCTTAACCGCAAGCTC
GATGCCGCCGCAGCCGCTGAGATCGTGAAAATCTTCACCGAAGTCATCATCGCCCCGGATGCCGATGAAGAAGCTATTGC
TCTTGTCGCCGCCAAGAAGAATCTGCGGCTTTTGCTCACCGGCGGGCTTCCAGATCCTCGCGCGGAAACCCTGACATTCC
GGCCCGTTGCTGGTGGTTTTCTCGTGCAGGGGCGAGACAGCGCGATCGTGGATGAGATGGAGCCGCGTGTCGTGACCAAA
AGAGCGCCGACCGAAGCGGAATGGCGCGATCTGAAATTTGCTTTCCGGGTCGCCAAACATGTGAAATCCAACGCCATCGT
TTACGCCAAGGATGGTGCGACGGTGGGGATTGGTGCGGGGCAGATGAGCCGTGTCGATTCCTCACGCATCGCCGCGATGA
AGGCGGAAGAGGCCGCGCGCGCGGCAGGCCTGCCCGAATCGCTCGCCATAGGGTCGGCGGTTGCTTCCGATGCCTTCTTT
CCCTTCCCCGATGGTCTGCTCGCTGCGGCGGCCGCGGGTGCGACAGCGGTCATTCAGCCGGGCGGTTCGGTGCGCGACAA
TGAAGTGATCGCTGCCGCTGACGAAGCAGGGCTCGCCATGGTTTTGACCGGTGTGCGGCATTTCCGGCATTAA

Upstream 100 bases:

>100_bases
TTGGCGCGAAAGTTATGAGTTTCGCCTGTTTCATCTGCCGCCATTTTGGGTTATGCGCGCCTTTTCCGCCTTTCTTTCCT
CCTTCCATCCAGGATCTGCC

Downstream 100 bases:

>100_bases
CCATAGGGAGAACCGTTGGTGGGCGGCCTGAGGCGCCATTAAGCTCCGGGCAAGGTTGCAAGGCTTAAAACGCTTTCTAT
TGATTGCGTAAACCTTGGAG

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MASELRRVERALISVSDKTGLVDFARALAALGIELVSTGGTSKALTEAGLAVRDVSDLTGFPELMDGRLKTLHPKVHGGL
LAIRENPEHEAAMLAHNIAPIDLLVVNLYPFEATIAKGAPFADCIENIDIGGPAMIRAASKNFNDVTVVIEPEDYAAILA
ELQGQNGATSLELRRKLAQKAFARTATYDATISNWFAKEIGETTPTYRAFGGRLIEALRYGENPHQAAAFYALPQGRAGV
TTARQVQGKALSYNNVNDTDAAFELVAEFDPSKQAAVAIIKHANPCGVAEAASLKEAYLLALRCDPVSAFGGIIALNRKL
DAAAAAEIVKIFTEVIIAPDADEEAIALVAAKKNLRLLLTGGLPDPRAETLTFRPVAGGFLVQGRDSAIVDEMEPRVVTK
RAPTEAEWRDLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAAMKAEEAARAAGLPESLAIGSAVASDAFF
PFPDGLLAAAAAGATAVIQPGGSVRDNEVIAAADEAGLAMVLTGVRHFRH

Sequences:

>Translated_530_residues
MASELRRVERALISVSDKTGLVDFARALAALGIELVSTGGTSKALTEAGLAVRDVSDLTGFPELMDGRLKTLHPKVHGGL
LAIRENPEHEAAMLAHNIAPIDLLVVNLYPFEATIAKGAPFADCIENIDIGGPAMIRAASKNFNDVTVVIEPEDYAAILA
ELQGQNGATSLELRRKLAQKAFARTATYDATISNWFAKEIGETTPTYRAFGGRLIEALRYGENPHQAAAFYALPQGRAGV
TTARQVQGKALSYNNVNDTDAAFELVAEFDPSKQAAVAIIKHANPCGVAEAASLKEAYLLALRCDPVSAFGGIIALNRKL
DAAAAAEIVKIFTEVIIAPDADEEAIALVAAKKNLRLLLTGGLPDPRAETLTFRPVAGGFLVQGRDSAIVDEMEPRVVTK
RAPTEAEWRDLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAAMKAEEAARAAGLPESLAIGSAVASDAFF
PFPDGLLAAAAAGATAVIQPGGSVRDNEVIAAADEAGLAMVLTGVRHFRH
>Mature_529_residues
ASELRRVERALISVSDKTGLVDFARALAALGIELVSTGGTSKALTEAGLAVRDVSDLTGFPELMDGRLKTLHPKVHGGLL
AIRENPEHEAAMLAHNIAPIDLLVVNLYPFEATIAKGAPFADCIENIDIGGPAMIRAASKNFNDVTVVIEPEDYAAILAE
LQGQNGATSLELRRKLAQKAFARTATYDATISNWFAKEIGETTPTYRAFGGRLIEALRYGENPHQAAAFYALPQGRAGVT
TARQVQGKALSYNNVNDTDAAFELVAEFDPSKQAAVAIIKHANPCGVAEAASLKEAYLLALRCDPVSAFGGIIALNRKLD
AAAAAEIVKIFTEVIIAPDADEEAIALVAAKKNLRLLLTGGLPDPRAETLTFRPVAGGFLVQGRDSAIVDEMEPRVVTKR
APTEAEWRDLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAAMKAEEAARAAGLPESLAIGSAVASDAFFP
FPDGLLAAAAAGATAVIQPGGSVRDNEVIAAADEAGLAMVLTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=473, Percent_Identity=41.4376321353066, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1790439, Length=546, Percent_Identity=55.1282051282051, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=34.1625207296849, Blast_Score=282, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=343, Percent_Identity=30.0291545189504, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=36.9978858350951, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=478, Percent_Identity=36.4016736401674, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=599, Percent_Identity=34.3906510851419, Blast_Score=284, Evalue=9e-77,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55881; Mature: 55750

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASELRRVERALISVSDKTGLVDFARALAALGIELVSTGGTSKALTEAGLAVRDVSDLTG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHEEECCCCHHHHHHCCCCEEEHHHHCC
FPELMDGRLKTLHPKVHGGLLAIRENPEHEAAMLAHNIAPIDLLVVNLYPFEATIAKGAP
CHHHHCCCHHHCCCHHCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCHHHHCCCCC
FADCIENIDIGGPAMIRAASKNFNDVTVVIEPEDYAAILAELQGQNGATSLELRRKLAQK
HHHHHHCCCCCCHHEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
AFARTATYDATISNWFAKEIGETTPTYRAFGGRLIEALRYGENPHQAAAFYALPQGRAGV
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCC
TTARQVQGKALSYNNVNDTDAAFELVAEFDPSKQAAVAIIKHANPCGVAEAASLKEAYLL
CHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHEEEEEECCCCCCCHHHHHHCEEEEE
ALRCDPVSAFGGIIALNRKLDAAAAAEIVKIFTEVIIAPDADEEAIALVAAKKNLRLLLT
EEECCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCEEEEEE
GGLPDPRAETLTFRPVAGGFLVQGRDSAIVDEMEPRVVTKRAPTEAEWRDLKFAFRVAKH
CCCCCCCCCEEEEEECCCCEEEECCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHH
VKSNAIVYAKDGATVGIGAGQMSRVDSSRIAAMKAEEAARAAGLPESLAIGSAVASDAFF
HCCCEEEEEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
PFPDGLLAAAAAGATAVIQPGGSVRDNEVIAAADEAGLAMVLTGVRHFRH
CCCCHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ASELRRVERALISVSDKTGLVDFARALAALGIELVSTGGTSKALTEAGLAVRDVSDLTG
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHEEECCCCHHHHHHCCCCEEEHHHHCC
FPELMDGRLKTLHPKVHGGLLAIRENPEHEAAMLAHNIAPIDLLVVNLYPFEATIAKGAP
CHHHHCCCHHHCCCHHCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCHHHHCCCCC
FADCIENIDIGGPAMIRAASKNFNDVTVVIEPEDYAAILAELQGQNGATSLELRRKLAQK
HHHHHHCCCCCCHHEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
AFARTATYDATISNWFAKEIGETTPTYRAFGGRLIEALRYGENPHQAAAFYALPQGRAGV
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCC
TTARQVQGKALSYNNVNDTDAAFELVAEFDPSKQAAVAIIKHANPCGVAEAASLKEAYLL
CHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHEEEEEECCCCCCCHHHHHHCEEEEE
ALRCDPVSAFGGIIALNRKLDAAAAAEIVKIFTEVIIAPDADEEAIALVAAKKNLRLLLT
EEECCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCEEEEEE
GGLPDPRAETLTFRPVAGGFLVQGRDSAIVDEMEPRVVTKRAPTEAEWRDLKFAFRVAKH
CCCCCCCCCEEEEEECCCCEEEECCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHH
VKSNAIVYAKDGATVGIGAGQMSRVDSSRIAAMKAEEAARAAGLPESLAIGSAVASDAFF
HCCCEEEEEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
PFPDGLLAAAAAGATAVIQPGGSVRDNEVIAAADEAGLAMVLTGVRHFRH
CCCCHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]