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The map label for this gene is glyA

Identifier: 182678210

GI number: 182678210

Start: 1421919

End: 1423220

Strand: Direct

Name: glyA

Synonym: Bind_1225

Alternate gene names: 182678210

Gene position: 1421919-1423220 (Clockwise)

Preceding gene: 182678207

Following gene: 182678211

Centisome position: 34.1

GC content: 58.99

Gene sequence:

>1302_bases
ATGAATGCCAAAGTCGAAGTCGGTCAGCCGGCGGCCAATTCCTTTTTCGCCGCGAATCTCGCCGATGCCGATCCTGAAAT
CGCCAAGGCGATCGAACTCGAACTCGGCCGCCAGCGGCATGAAATCGAATTGATCGCGTCGGAAAATATCGTCTCCAAGG
CCGTGCTCGAGGCGCAAGGCTCGATCATGACGAATAAATATGCCGAAGGCTATCCGGGCAAACGCTATTATGGCGGCTGT
CAATTCGTTGATATTGCCGAAAATCTGGCGATCGAACGGGTCCGCAAATTGTTCGACTGCCAATTCGCCAATGTGCAGCC
CAATTCCGGCAGTCAGGCGAATCAGGCGGTCTTTCTCGCCCTTTTGCAGCCGGGCGACGTTTTCATGGGCCTCGATCTCG
CGGCCGGAGGCCATTTGACTCATGGCTCGCCGGTTAATTTGTCTGGTAAATGGTTCAAGGCGGTCTCCTATGGCGTGCGT
CAGTCAGACCATTTGATCGATATGGACGCGGTCGAGGCTCTCGCCAAGGAACATAAGCCGAAACTGATCATCGCCGGCGG
CTCTGCCTATCCCCGCCATTGGGATTTCGCCCGTTTTCGCGCGATCGCGGATTCGGTCGGCGCCTATTTCTTCGTCGATA
TGGCGCATTTCGCCGGTCTCGTCGCCGGTGGCGCGCATCCCTCTCCCTTTCCGCATGCGCATGTCGTCACCTCGACCACG
CACAAAACCTTGCGCGGCCCGCGCGGTGGCCTTGTCCTCACCAATGACGCCGACATTGCGAAGAAGATCAATTCCGCCGT
TTTCCCCGGTCTTCAGGGTGGCCCTTTGATGCATGTCATCGCCGCCAAGGCGGTTGCTTTTGGCGAGGCCCTGCGGCCCG
ATTTCCGTTTGTATGCGCAGCAGGTGGTGGTGAATGCGGGCACGCTCGCCTCGCGGCTGGTCGAAAAAGGATTCGCGATT
TCGTCCGGTGGCACGGACAATCATTTGATGCTCGTCGATCTCCGGCCAAAGCAATTGACCGGCAAGGCGGCGGAAGCAGC
CCTTGGGCGCGCCTCGATCACCTGCAACAAAAACGGCGTGCCTTTCGATACGGCGAGCCCTTTCGTGACCTCAGGGATCA
GGCTCGGCTCGCCGGCGGCGACCTCTCGTGGTTTCGGCACCAAGGAATTCCAGGACGTGGCGGATCTCATCGCCGAAACC
CTGGATGGGCTCGCCAAGAATGGCGAGGAAGGCAATGCTGCCGTTGAGGCCAGTGTCAAGGAGCGGGCTATTGCCCTGAC
CCAGCGCTTCCCCATCTATTGA

Upstream 100 bases:

>100_bases
CATATCCCTGGCCGGTGCCGGCGTTCTCGAAATTTTCGGACGTCCTTCCTATTTTGCAATGAATAGCGCCGCCAAACCTT
GGATGACCGGGAGTATCGCC

Downstream 100 bases:

>100_bases
GCGTAGCAACGCTTATTCTTATACCAACGGTCATGATCAATGACCGTTGGACCACTCTTGAATTTTCGCCATCGCTTTGA
AAAAGCGAAAATTCAAGAAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNAKVEVGQPAANSFFAANLADADPEIAKAIELELGRQRHEIELIASENIVSKAVLEAQGSIMTNKYAEGYPGKRYYGGC
QFVDIAENLAIERVRKLFDCQFANVQPNSGSQANQAVFLALLQPGDVFMGLDLAAGGHLTHGSPVNLSGKWFKAVSYGVR
QSDHLIDMDAVEALAKEHKPKLIIAGGSAYPRHWDFARFRAIADSVGAYFFVDMAHFAGLVAGGAHPSPFPHAHVVTSTT
HKTLRGPRGGLVLTNDADIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPDFRLYAQQVVVNAGTLASRLVEKGFAI
SSGGTDNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGVPFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVADLIAET
LDGLAKNGEEGNAAVEASVKERAIALTQRFPIY

Sequences:

>Translated_433_residues
MNAKVEVGQPAANSFFAANLADADPEIAKAIELELGRQRHEIELIASENIVSKAVLEAQGSIMTNKYAEGYPGKRYYGGC
QFVDIAENLAIERVRKLFDCQFANVQPNSGSQANQAVFLALLQPGDVFMGLDLAAGGHLTHGSPVNLSGKWFKAVSYGVR
QSDHLIDMDAVEALAKEHKPKLIIAGGSAYPRHWDFARFRAIADSVGAYFFVDMAHFAGLVAGGAHPSPFPHAHVVTSTT
HKTLRGPRGGLVLTNDADIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPDFRLYAQQVVVNAGTLASRLVEKGFAI
SSGGTDNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGVPFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVADLIAET
LDGLAKNGEEGNAAVEASVKERAIALTQRFPIY
>Mature_433_residues
MNAKVEVGQPAANSFFAANLADADPEIAKAIELELGRQRHEIELIASENIVSKAVLEAQGSIMTNKYAEGYPGKRYYGGC
QFVDIAENLAIERVRKLFDCQFANVQPNSGSQANQAVFLALLQPGDVFMGLDLAAGGHLTHGSPVNLSGKWFKAVSYGVR
QSDHLIDMDAVEALAKEHKPKLIIAGGSAYPRHWDFARFRAIADSVGAYFFVDMAHFAGLVAGGAHPSPFPHAHVVTSTT
HKTLRGPRGGLVLTNDADIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPDFRLYAQQVVVNAGTLASRLVEKGFAI
SSGGTDNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGVPFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVADLIAET
LDGLAKNGEEGNAAVEASVKERAIALTQRFPIY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=49.009900990099, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=49.009900990099, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=49.009900990099, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=49.009900990099, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=48.2673267326733, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI22547186, Length=434, Percent_Identity=46.7741935483871, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI22547189, Length=420, Percent_Identity=44.5238095238095, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=58.5131894484413, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144729, Length=420, Percent_Identity=48.5714285714286, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144732, Length=420, Percent_Identity=48.5714285714286, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319739, Length=419, Percent_Identity=43.9140811455847, Blast_Score=352, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=415, Percent_Identity=41.6867469879518, Blast_Score=324, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640005, Length=413, Percent_Identity=46.4891041162228, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI221329721, Length=413, Percent_Identity=46.4891041162228, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_BEII9 (B2IJJ3)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832356.1
- ProteinModelPortal:   B2IJJ3
- GeneID:   6198213
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1225
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45992; Mature: 45992

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKVEVGQPAANSFFAANLADADPEIAKAIELELGRQRHEIELIASENIVSKAVLEAQG
CCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCC
SIMTNKYAEGYPGKRYYGGCQFVDIAENLAIERVRKLFDCQFANVQPNSGSQANQAVFLA
CEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCEEEEE
LLQPGDVFMGLDLAAGGHLTHGSPVNLSGKWFKAVSYGVRQSDHLIDMDAVEALAKEHKP
EECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECHHHHHHHHHHCCC
KLIIAGGSAYPRHWDFARFRAIADSVGAYFFVDMAHFAGLVAGGAHPSPFPHAHVVTSTT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
HKTLRGPRGGLVLTNDADIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPDFRLYAQ
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
QVVVNAGTLASRLVEKGFAISSGGTDNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGV
HHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCHHHCCHHHHHHCCCEEEEECCCCC
PFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVADLIAETLDGLAKNGEEGNAAVEASVK
CCCCCCCHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
ERAIALTQRFPIY
HHHHHHHHCCCCC
>Mature Secondary Structure
MNAKVEVGQPAANSFFAANLADADPEIAKAIELELGRQRHEIELIASENIVSKAVLEAQG
CCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCC
SIMTNKYAEGYPGKRYYGGCQFVDIAENLAIERVRKLFDCQFANVQPNSGSQANQAVFLA
CEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCEEEEE
LLQPGDVFMGLDLAAGGHLTHGSPVNLSGKWFKAVSYGVRQSDHLIDMDAVEALAKEHKP
EECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECHHHHHHHHHHCCC
KLIIAGGSAYPRHWDFARFRAIADSVGAYFFVDMAHFAGLVAGGAHPSPFPHAHVVTSTT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
HKTLRGPRGGLVLTNDADIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPDFRLYAQ
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
QVVVNAGTLASRLVEKGFAISSGGTDNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGV
HHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCHHHCCHHHHHHCCCEEEEECCCCC
PFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVADLIAETLDGLAKNGEEGNAAVEASVK
CCCCCCCHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
ERAIALTQRFPIY
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA