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The map label for this gene is vanX [H]

Identifier: 182678191

GI number: 182678191

Start: 1400426

End: 1401109

Strand: Direct

Name: vanX [H]

Synonym: Bind_1206

Alternate gene names: 182678191

Gene position: 1400426-1401109 (Clockwise)

Preceding gene: 182678184

Following gene: 182678195

Centisome position: 33.58

GC content: 57.6

Gene sequence:

>684_bases
ATGGGGAAACGACTTTTGTCCGCTTTCGCAATGATCATGCTCTCTTGCGCGGGGGTGTCCTCCGCCGTCGCCAAGGGGGT
TGAGAAAGAAACGCTTCCTCCGCCGGATTTCGTGCGATTGCAGGATGTTGCGCCTTCCATCGCGCAGGATATGCGCTATG
CGGGCTCCAATAATTTTACAGGCAGGCCCGTGCCGGGTTATGCCGCCGGCACATGTTGGCTGCGGCGCGAAGCAGCCTTG
GCCCTTGCCGCCGTGCAGAAAGATGCTGAAGCAGAGGGACTCGGGCTCGTCGTTTTTGATTGTTTTCGGCCGCAAAGGGC
GAGCAATGCTTTTCTCGCCTGGGCGCAAGACCCCACGGATCAGGCGATGAAGGCAGCCTATTATCCGGGAATCGACAAGC
GTGTATTGTTCGATCAAGGCTATATCGCCGCGAAATCCACGCATTCGACCGGGCTTGCTGTCGATCTCGGCTTTATCGGC
CGCGATTTCGGCACCCCTTTCGATCTCTTCGACGCGGCCTCGGCCACGGACTTTCAGGCCATCGACGCTGAAGCCAAGGC
CAATCGTGACAAGCTTCTGAGCCTCATGCGCAAGCATGGGTTCGAGAACCTGCCCAATGAATGGTGGCATTTCAATCTGC
CTGGCGCCGCCAAGGCTGAGTTTTTTGATTTCGAAGTCCGATAG

Upstream 100 bases:

>100_bases
TGTGGCCTTTCGCGATGGGCGGTGCAAGAGAGGGAATGCGGTGCAGGTTGGGTATCGCAGCAGGTTATCCTGCGTCTCGC
CTGCGATCTGGGAATTGGGG

Downstream 100 bases:

>100_bases
GCTGTCAAGGATCGACTTGTTTCTCCGTTTCGATCCTGCCTCCGAGACGCCAGGCGCCAATAAGGCTGCTCAGCACGAGA
ATTCCCCATAAAGACCATAA

Product: peptidase M15D vanX D-ala-D-ala dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MGKRLLSAFAMIMLSCAGVSSAVAKGVEKETLPPPDFVRLQDVAPSIAQDMRYAGSNNFTGRPVPGYAAGTCWLRREAAL
ALAAVQKDAEAEGLGLVVFDCFRPQRASNAFLAWAQDPTDQAMKAAYYPGIDKRVLFDQGYIAAKSTHSTGLAVDLGFIG
RDFGTPFDLFDAASATDFQAIDAEAKANRDKLLSLMRKHGFENLPNEWWHFNLPGAAKAEFFDFEVR

Sequences:

>Translated_227_residues
MGKRLLSAFAMIMLSCAGVSSAVAKGVEKETLPPPDFVRLQDVAPSIAQDMRYAGSNNFTGRPVPGYAAGTCWLRREAAL
ALAAVQKDAEAEGLGLVVFDCFRPQRASNAFLAWAQDPTDQAMKAAYYPGIDKRVLFDQGYIAAKSTHSTGLAVDLGFIG
RDFGTPFDLFDAASATDFQAIDAEAKANRDKLLSLMRKHGFENLPNEWWHFNLPGAAKAEFFDFEVR
>Mature_226_residues
GKRLLSAFAMIMLSCAGVSSAVAKGVEKETLPPPDFVRLQDVAPSIAQDMRYAGSNNFTGRPVPGYAAGTCWLRREAALA
LAAVQKDAEAEGLGLVVFDCFRPQRASNAFLAWAQDPTDQAMKAAYYPGIDKRVLFDQGYIAAKSTHSTGLAVDLGFIGR
DFGTPFDLFDAASATDFQAIDAEAKANRDKLLSLMRKHGFENLPNEWWHFNLPGAAKAEFFDFEVR

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=188, Percent_Identity=31.3829787234043, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 24764; Mature: 24633

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLSAFAMIMLSCAGVSSAVAKGVEKETLPPPDFVRLQDVAPSIAQDMRYAGSNNFT
CCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC
GRPVPGYAAGTCWLRREAALALAAVQKDAEAEGLGLVVFDCFRPQRASNAFLAWAQDPTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHH
QAMKAAYYPGIDKRVLFDQGYIAAKSTHSTGLAVDLGFIGRDFGTPFDLFDAASATDFQA
HHHHHHHCCCCCCEEEECCCEEEECCCCCCCEEEEECHHHHCCCCCHHHHHCCCCCCCHH
IDAEAKANRDKLLSLMRKHGFENLPNEWWHFNLPGAAKAEFFDFEVR
HCCHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
GKRLLSAFAMIMLSCAGVSSAVAKGVEKETLPPPDFVRLQDVAPSIAQDMRYAGSNNFT
CHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC
GRPVPGYAAGTCWLRREAALALAAVQKDAEAEGLGLVVFDCFRPQRASNAFLAWAQDPTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHH
QAMKAAYYPGIDKRVLFDQGYIAAKSTHSTGLAVDLGFIGRDFGTPFDLFDAASATDFQA
HHHHHHHCCCCCCEEEECCCEEEECCCCCCCEEEEECHHHHCCCCCHHHHHCCCCCCCHH
IDAEAKANRDKLLSLMRKHGFENLPNEWWHFNLPGAAKAEFFDFEVR
HCCHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]