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The map label for this gene is leuB2 [H]

Identifier: 182678167

GI number: 182678167

Start: 1370623

End: 1371732

Strand: Direct

Name: leuB2 [H]

Synonym: Bind_1182

Alternate gene names: 182678167

Gene position: 1370623-1371732 (Clockwise)

Preceding gene: 182678166

Following gene: 182678168

Centisome position: 32.87

GC content: 58.38

Gene sequence:

>1110_bases
ATGGCATATAAGCTTTTCGTTCTGCCGGGTGATGGAATCGGCCCAGAGGTCATGGCCGAGGTCGAAAAGATCGCGAGCTT
TTTCACGCAAGAGGGCAGTGCGGCCTTCGAGATCGAGAAAGGACTCGTCGGTGGCTCCGCCTATGACGCCCATGGCGTGG
CGATCAGCGATGCCGACATGGCTCGCGCCGATGCGGCTGACGCTGTTCTGTTAGCCGCGGTGGGAGGCCCCAAATGGGAT
GCGGTGCCTTATGAGGTCCGCCCCGAAGCTGGTCTCTTGCGTCTGCGCAAGGATCTGAAGCTTTTCGCTAATCTGCGCCC
AGCCGTGTGCTATCCAGCGCTCGCCGACGCGTCATCCTTGAAGCGCGAGCTCGTCGATGGGCTCGACCTGATGATCGTGC
GTGAATTGACGGGTGGTGTCTATTTCGGTGAGCCGAAGCAGATCATCGATCTCGGCAATGGCCAGCAACGCGCGATCGAT
ACGCAGGTCTATGAGACCTATGAGATCGAGCGGATCGGCCGCATCGCTTTCGAGCTTGCCCGCAAACGGCGTAAGAAAGT
GACCTCGTCCGAAAAGCGCAATGTCATGAGAACCGGTGTGCTCTGGCATCAGATCATCAACCGTCTGCATGATCAGGAAT
TTCCCGATGTCACGCTCGAACATCAGCTCGCTGATGCACTCGGCATGCAGCTCGTGCGGGCGCCGAAGCAATTCGATGTC
ATTGTGACGGATAATCTGTTTGGCGACATGTTGTCCGATGTCGCGGCCATGTTGACGGGGTCGCTCGGCATGCTGCCTTC
GGCCTCGCTTGGCGAGGTTGATAGCCAGACCGGCAAGCGCAAGGCTCTTTATGAACCTGTGCATGGTTCGGCGCCCGATA
TTGCGGGCAAGGGAATTGCCAATCCAATCGCCATGATCGCCTCCTTCGGCATGGCCTTGCGTTATTCCTTCGATCTTGGC
GCTCTGGCGGACAGAATTGACAATGCGATCGCCGCGACACTCGCCAAGGGTCTGCGTACCGCCGATATCAAGGGAAGCAG
CACGGAGCCTACGGTTTCGACGACGCAAATGGGCGATGCCATTCTGAAGGAATTGCAGGGCGCCGCGTAA

Upstream 100 bases:

>100_bases
AAAATAGGTTTAAAGGAGCGGCCACCTTGACCGAGGTGACCGCTCCTGCCTTTTTGCAGACCTGACATTCCAACGGATAG
GCCCTCAGGGAAATAACAGC

Downstream 100 bases:

>100_bases
GCGAAACATCCGTTTGTATCAATGCGGATGCATGAGAACAGCGGGTTCCAAAAGGACCCGCTGATACACTCACGATCACA
TCAGAAGTGACACCGAGCGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH 2; Beta-IPM dehydrogenase 2; IMDH 2 [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAYKLFVLPGDGIGPEVMAEVEKIASFFTQEGSAAFEIEKGLVGGSAYDAHGVAISDADMARADAADAVLLAAVGGPKWD
AVPYEVRPEAGLLRLRKDLKLFANLRPAVCYPALADASSLKRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQQRAID
TQVYETYEIERIGRIAFELARKRRKKVTSSEKRNVMRTGVLWHQIINRLHDQEFPDVTLEHQLADALGMQLVRAPKQFDV
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGEVDSQTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFGMALRYSFDLG
ALADRIDNAIAATLAKGLRTADIKGSSTEPTVSTTQMGDAILKELQGAA

Sequences:

>Translated_369_residues
MAYKLFVLPGDGIGPEVMAEVEKIASFFTQEGSAAFEIEKGLVGGSAYDAHGVAISDADMARADAADAVLLAAVGGPKWD
AVPYEVRPEAGLLRLRKDLKLFANLRPAVCYPALADASSLKRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQQRAID
TQVYETYEIERIGRIAFELARKRRKKVTSSEKRNVMRTGVLWHQIINRLHDQEFPDVTLEHQLADALGMQLVRAPKQFDV
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGEVDSQTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFGMALRYSFDLG
ALADRIDNAIAATLAKGLRTADIKGSSTEPTVSTTQMGDAILKELQGAA
>Mature_368_residues
AYKLFVLPGDGIGPEVMAEVEKIASFFTQEGSAAFEIEKGLVGGSAYDAHGVAISDADMARADAADAVLLAAVGGPKWDA
VPYEVRPEAGLLRLRKDLKLFANLRPAVCYPALADASSLKRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQQRAIDT
QVYETYEIERIGRIAFELARKRRKKVTSSEKRNVMRTGVLWHQIINRLHDQEFPDVTLEHQLADALGMQLVRAPKQFDVI
VTDNLFGDMLSDVAAMLTGSLGMLPSASLGEVDSQTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFGMALRYSFDLGA
LADRIDNAIAATLAKGLRTADIKGSSTEPTVSTTQMGDAILKELQGAA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=348, Percent_Identity=33.6206896551724, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI28178821, Length=378, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178816, Length=360, Percent_Identity=28.6111111111111, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=29.2613636363636, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI28178838, Length=337, Percent_Identity=29.080118694362, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178819, Length=202, Percent_Identity=32.6732673267327, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=49.5890410958904, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1788101, Length=370, Percent_Identity=33.5135135135135, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787381, Length=264, Percent_Identity=27.6515151515151, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=33.4277620396601, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=378, Percent_Identity=30.1587301587302, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=364, Percent_Identity=31.8681318681319, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=369, Percent_Identity=30.3523035230352, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=41.4634146341463, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=32.1621621621622, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=33.0623306233062, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=370, Percent_Identity=29.4594594594595, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643270, Length=351, Percent_Identity=36.1823361823362, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24643268, Length=351, Percent_Identity=36.1823361823362, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24661184, Length=376, Percent_Identity=29.7872340425532, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078633, Length=372, Percent_Identity=27.6881720430108, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650122, Length=372, Percent_Identity=27.6881720430108, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078637, Length=376, Percent_Identity=27.9255319148936, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078635, Length=376, Percent_Identity=27.9255319148936, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=372, Percent_Identity=28.2258064516129, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=27.7620396600567, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=27.7620396600567, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130355, Length=223, Percent_Identity=25.5605381165919, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39649; Mature: 39518

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKLFVLPGDGIGPEVMAEVEKIASFFTQEGSAAFEIEKGLVGGSAYDAHGVAISDADM
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHH
ARADAADAVLLAAVGGPKWDAVPYEVRPEAGLLRLRKDLKLFANLRPAVCYPALADASSL
HHHCHHCEEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHH
KRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQQRAIDTQVYETYEIERIGRIAFELA
HHHHHCCHHHHHHHHHHCCEEECCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKRRKKVTSSEKRNVMRTGVLWHQIINRLHDQEFPDVTLEHQLADALGMQLVRAPKQFDV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGEVDSQTGKRKALYEPVHGSAPDIAGKGIA
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCCCCC
NPIAMIASFGMALRYSFDLGALADRIDNAIAATLAKGLRTADIKGSSTEPTVSTTQMGDA
HHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHH
ILKELQGAA
HHHHHCCCC
>Mature Secondary Structure 
AYKLFVLPGDGIGPEVMAEVEKIASFFTQEGSAAFEIEKGLVGGSAYDAHGVAISDADM
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHH
ARADAADAVLLAAVGGPKWDAVPYEVRPEAGLLRLRKDLKLFANLRPAVCYPALADASSL
HHHCHHCEEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHH
KRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQQRAIDTQVYETYEIERIGRIAFELA
HHHHHCCHHHHHHHHHHCCEEECCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKRRKKVTSSEKRNVMRTGVLWHQIINRLHDQEFPDVTLEHQLADALGMQLVRAPKQFDV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGEVDSQTGKRKALYEPVHGSAPDIAGKGIA
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCCCCC
NPIAMIASFGMALRYSFDLGALADRIDNAIAATLAKGLRTADIKGSSTEPTVSTTQMGDA
HHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHH
ILKELQGAA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]