Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 182678107

GI number: 182678107

Start: 1299137

End: 1300066

Strand: Direct

Name: metF [H]

Synonym: Bind_1122

Alternate gene names: 182678107

Gene position: 1299137-1300066 (Clockwise)

Preceding gene: 182678106

Following gene: 182678108

Centisome position: 31.15

GC content: 58.28

Gene sequence:

>930_bases
ATGAGCGATACTATGACGCTTCAATCAATCGATTCATCGCCTCGCGCCAGTCGCGGAACTTGTGAAGGCATGAGCGTCTC
CTTCGAATTTTTTCCGCCCAAAACCGAGGCGATGGAAGCGACCCTGTGGGAATCGATCAATCGCCTGGCGCCTTTGCAGC
CGAGCTTTGTCTCGGTGACCTATGGCGCTGGTGGCTCGACGCGCGAGCGGACCCATGCGACCGTTGCTCGCATCGTGAAG
GAAACGCCGATAAAGCCGGCGGCGCATCTGACCTGTGTGGCGGCAAGTTGCGCGGAAGTCGATGCGGTCGCCAAGGCCTA
TTGGGAGGCAGGTGTCCGTCATATCGTGGCTTTGCGTGGTGATCCCCTGGAAGGTATTGGGACGGCCTTTCATCCACATC
CGCAAGGCTATGCGCATTCGACGGATCTCGTGGCAGGCCTGCGCAAGATCGCCGATTTCGAAATTTCGGTCTCCGCCTAT
CCCGAGAAACATCCCGAAAGCGCGTCGATCGAATCCGATCTCGACGTTCTGCAGGCCAAGATCGATGCCGGCGCGACCCG
CGCCATCACCCAATTCTTTTTCGAGAATGACACCTACCTGCGCTATCTCGACAAGGTGCGGGCGCGCGGGATCACCATTC
CGATCCTGCCGGGCATTGTGCCGGTACAGAATTTCAAACAGACCGCGAATTTCGCCAAAAAGGCCGGCGCGAGTATTCCG
CCTTGGCTCGCTGAACGGTTCGAGGGGCTTGACGATGATCCCCAGACGCGCCGATTGATTGCTGCTACGGTCGCCGCCGA
ACAAGTGCTCGATCTCGTCGATCGCGGTGTGCGGGATTTCCATTTTTATACGATGAATCGCGCCGATCTCGTTTATGCGA
TCTGCCACCTTCTGGGGCTGCGGCCGAAACTCGAGAAAGAAGCTGCCTGA

Upstream 100 bases:

>100_bases
AGGGTGGCAAGCTGACCGTCTCGCTCTGGCTCGGCCACCATCGATCCGCCCGCCCCAATGACCGGCAGGGTTCCAAAACC
TCGCCCGCACGAGAAATAGC

Downstream 100 bases:

>100_bases
ATGAGCCAGTCCCCTTTGAATGGTCCCGCGACGACCAAAGCGCTTCTGGAAGCGGCCGCCGAGCGCGTTCTTGTGCTTGA
TGGTGCCATGGGCACGATGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSDTMTLQSIDSSPRASRGTCEGMSVSFEFFPPKTEAMEATLWESINRLAPLQPSFVSVTYGAGGSTRERTHATVARIVK
ETPIKPAAHLTCVAASCAEVDAVAKAYWEAGVRHIVALRGDPLEGIGTAFHPHPQGYAHSTDLVAGLRKIADFEISVSAY
PEKHPESASIESDLDVLQAKIDAGATRAITQFFFENDTYLRYLDKVRARGITIPILPGIVPVQNFKQTANFAKKAGASIP
PWLAERFEGLDDDPQTRRLIAATVAAEQVLDLVDRGVRDFHFYTMNRADLVYAICHLLGLRPKLEKEAA

Sequences:

>Translated_309_residues
MSDTMTLQSIDSSPRASRGTCEGMSVSFEFFPPKTEAMEATLWESINRLAPLQPSFVSVTYGAGGSTRERTHATVARIVK
ETPIKPAAHLTCVAASCAEVDAVAKAYWEAGVRHIVALRGDPLEGIGTAFHPHPQGYAHSTDLVAGLRKIADFEISVSAY
PEKHPESASIESDLDVLQAKIDAGATRAITQFFFENDTYLRYLDKVRARGITIPILPGIVPVQNFKQTANFAKKAGASIP
PWLAERFEGLDDDPQTRRLIAATVAAEQVLDLVDRGVRDFHFYTMNRADLVYAICHLLGLRPKLEKEAA
>Mature_308_residues
SDTMTLQSIDSSPRASRGTCEGMSVSFEFFPPKTEAMEATLWESINRLAPLQPSFVSVTYGAGGSTRERTHATVARIVKE
TPIKPAAHLTCVAASCAEVDAVAKAYWEAGVRHIVALRGDPLEGIGTAFHPHPQGYAHSTDLVAGLRKIADFEISVSAYP
EKHPESASIESDLDVLQAKIDAGATRAITQFFFENDTYLRYLDKVRARGITIPILPGIVPVQNFKQTANFAKKAGASIPP
WLAERFEGLDDDPQTRRLIAATVAAEQVLDLVDRGVRDFHFYTMNRADLVYAICHLLGLRPKLEKEAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=32.7402135231317, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1790377, Length=281, Percent_Identity=53.0249110320285, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25147436, Length=289, Percent_Identity=31.4878892733564, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=289, Percent_Identity=31.4878892733564, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=296, Percent_Identity=34.1216216216216, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=292, Percent_Identity=29.4520547945205, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24662658, Length=276, Percent_Identity=25.3623188405797, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33812; Mature: 33681

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTMTLQSIDSSPRASRGTCEGMSVSFEFFPPKTEAMEATLWESINRLAPLQPSFVSVT
CCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
YGAGGSTRERTHATVARIVKETPIKPAAHLTCVAASCAEVDAVAKAYWEAGVRHIVALRG
ECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DPLEGIGTAFHPHPQGYAHSTDLVAGLRKIADFEISVSAYPEKHPESASIESDLDVLQAK
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHH
IDAGATRAITQFFFENDTYLRYLDKVRARGITIPILPGIVPVQNFKQTANFAKKAGASIP
HCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PWLAERFEGLDDDPQTRRLIAATVAAEQVLDLVDRGVRDFHFYTMNRADLVYAICHLLGL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHCC
RPKLEKEAA
CCCHHHCCC
>Mature Secondary Structure 
SDTMTLQSIDSSPRASRGTCEGMSVSFEFFPPKTEAMEATLWESINRLAPLQPSFVSVT
CCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
YGAGGSTRERTHATVARIVKETPIKPAAHLTCVAASCAEVDAVAKAYWEAGVRHIVALRG
ECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DPLEGIGTAFHPHPQGYAHSTDLVAGLRKIADFEISVSAYPEKHPESASIESDLDVLQAK
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHH
IDAGATRAITQFFFENDTYLRYLDKVRARGITIPILPGIVPVQNFKQTANFAKKAGASIP
HCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PWLAERFEGLDDDPQTRRLIAATVAAEQVLDLVDRGVRDFHFYTMNRADLVYAICHLLGL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHCC
RPKLEKEAA
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]