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The map label for this gene is purD [H]

Identifier: 182677999

GI number: 182677999

Start: 1163288

End: 1164553

Strand: Direct

Name: purD [H]

Synonym: Bind_1013

Alternate gene names: 182677999

Gene position: 1163288-1164553 (Clockwise)

Preceding gene: 182677998

Following gene: 182678000

Centisome position: 27.9

GC content: 60.98

Gene sequence:

>1266_bases
ATGAACGTCCTTCTCATCGGCTCCGGCGGGCGCGAACATGCGATCGCCAAAACTCTCGCCAAAAGCCCTTTGCTCTCGAC
CCTCTTCGTTGCGCCGGGCAATCCCGGCATGATGGAGATCGCCGAACCCGTCGTGCTCAAAATCGAGAATCATCAGGCGG
TCATCGATTTCTGCCGCCTCCAGGCGATCGGCCTTGTCGTGATCGGCCCCGAGGTGCCGCTCGTCGCGGGGCTTACCGAT
CCGCTCGTTGCGGCGGGCATTAAAGTTTTTGGTCCGAGCCAAGCGGCCGCTCAGCTCGAAGGATCAAAAAATTTCACGAA
ACAATTCTGCTTGGAGCATGGGATTCCGACGGCGCAAGCCCAAAGCTTCGATGAGGCGGCGCCGGCCAAAGCCTATGCCG
CGACAAGACCCCTGCCGATCGTCGTTAAGGCGGATGGCCTCGCTGCCGGTAAGGGCGTCGTCGTGGCGGCGACCCATGCG
GAGGCGGAGGCTGCGATCGATCAGATTTTTGCGGGCGGAATCGGTGGCAATGGCTTGCTCATCGAGGATTTTCTGGAAGG
CGAGGAAGCCTCCTTCTTCGCGCTCTGTGATGGCACCAGCGCTTTACCCTTTGCCTCGGCGCAGGATCATAAACGGGTGG
GCGAGGGTGATACGGGCGCCAATACCGGTGGCATGGGGGCCTATTCGCCAGCGCCTGTCATGGATGCGGCCATGACCGAA
AGGGTCATCGATGAGATCATCACACCGACGGTCAAAGGCATGGCGGCACGCGGGACGCCCTTCAAGGGCATGTTATTCGC
GGGGCTGATGATCGGGCCCGCTGGGCCGAAGCTGATCGAATATAATGTCCGTTTCGGTGATCCGGAAACGCAGGTCATGC
TGCCAAGATTGCAAGACGATCTTCTCGCCCTGCTTCTTGCCTGCGCGGAGGGTCACCTGCCGCAAAAGCCTCTGCATTTT
GCCGATGAAGTCGCACTGACCGTCGTGCTCGCCGCCAAGGGCTATCCCGAAGCGCCGCAAAAGGGCAGCGAGATTCGGGG
CGTGGAAAAGGCGGCCAGTCTTCCTGGTGTCAGCCTAACTCATGCCGGCACGCGGCGCGAAGGTACGAAACTCCTGGCTG
ATGGAGGCCGCGTCTTGAATGTCACGGGCCTTGGCAAGGATGTGGCGGAAGCGCGGGAGCGCGCCTATGCGGCTGTCGAT
CTCATCGATTGGCCTGAAGGATTTTGCCGCCACGACATCGGATGGCGCGCGCTGCAAAGGAACTGA

Upstream 100 bases:

>100_bases
CGGCGCACTGGTTTTTCAAATTCTGTGGATTGTGCGCCATTATAATGTCATGGCTTGAAAGCTCTTTTTCGTCGTCCTCG
ATCTTCGAATATCCAGGCTC

Downstream 100 bases:

>100_bases
CATAAATCCGCTTAAGGGTGCTGGACAACGGGACGTCCAGTCCACTATAAGGCCCCAGGTTCGAAGCAACGTCTTGAGAC
AGAAGATTCGTTCCTGCCCA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNVLLIGSGGREHAIAKTLAKSPLLSTLFVAPGNPGMMEIAEPVVLKIENHQAVIDFCRLQAIGLVVIGPEVPLVAGLTD
PLVAAGIKVFGPSQAAAQLEGSKNFTKQFCLEHGIPTAQAQSFDEAAPAKAYAATRPLPIVVKADGLAAGKGVVVAATHA
EAEAAIDQIFAGGIGGNGLLIEDFLEGEEASFFALCDGTSALPFASAQDHKRVGEGDTGANTGGMGAYSPAPVMDAAMTE
RVIDEIITPTVKGMAARGTPFKGMLFAGLMIGPAGPKLIEYNVRFGDPETQVMLPRLQDDLLALLLACAEGHLPQKPLHF
ADEVALTVVLAAKGYPEAPQKGSEIRGVEKAASLPGVSLTHAGTRREGTKLLADGGRVLNVTGLGKDVAEARERAYAAVD
LIDWPEGFCRHDIGWRALQRN

Sequences:

>Translated_421_residues
MNVLLIGSGGREHAIAKTLAKSPLLSTLFVAPGNPGMMEIAEPVVLKIENHQAVIDFCRLQAIGLVVIGPEVPLVAGLTD
PLVAAGIKVFGPSQAAAQLEGSKNFTKQFCLEHGIPTAQAQSFDEAAPAKAYAATRPLPIVVKADGLAAGKGVVVAATHA
EAEAAIDQIFAGGIGGNGLLIEDFLEGEEASFFALCDGTSALPFASAQDHKRVGEGDTGANTGGMGAYSPAPVMDAAMTE
RVIDEIITPTVKGMAARGTPFKGMLFAGLMIGPAGPKLIEYNVRFGDPETQVMLPRLQDDLLALLLACAEGHLPQKPLHF
ADEVALTVVLAAKGYPEAPQKGSEIRGVEKAASLPGVSLTHAGTRREGTKLLADGGRVLNVTGLGKDVAEARERAYAAVD
LIDWPEGFCRHDIGWRALQRN
>Mature_421_residues
MNVLLIGSGGREHAIAKTLAKSPLLSTLFVAPGNPGMMEIAEPVVLKIENHQAVIDFCRLQAIGLVVIGPEVPLVAGLTD
PLVAAGIKVFGPSQAAAQLEGSKNFTKQFCLEHGIPTAQAQSFDEAAPAKAYAATRPLPIVVKADGLAAGKGVVVAATHA
EAEAAIDQIFAGGIGGNGLLIEDFLEGEEASFFALCDGTSALPFASAQDHKRVGEGDTGANTGGMGAYSPAPVMDAAMTE
RVIDEIITPTVKGMAARGTPFKGMLFAGLMIGPAGPKLIEYNVRFGDPETQVMLPRLQDDLLALLLACAEGHLPQKPLHF
ADEVALTVVLAAKGYPEAPQKGSEIRGVEKAASLPGVSLTHAGTRREGTKLLADGGRVLNVTGLGKDVAEARERAYAAVD
LIDWPEGFCRHDIGWRALQRN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=44.2080378250591, Blast_Score=337, Evalue=8e-93,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=44.2080378250591, Blast_Score=337, Evalue=8e-93,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=44.2080378250591, Blast_Score=337, Evalue=8e-93,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=44.1037735849057, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=51.9906323185012, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=40.6175771971496, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=41.0835214446953, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI62471619, Length=405, Percent_Identity=43.9506172839506, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24582400, Length=405, Percent_Identity=43.9506172839506, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43873; Mature: 43873

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLLIGSGGREHAIAKTLAKSPLLSTLFVAPGNPGMMEIAEPVVLKIENHQAVIDFCRL
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCEEEEECCCHHHHHHHHH
QAIGLVVIGPEVPLVAGLTDPLVAAGIKVFGPSQAAAQLEGSKNFTKQFCLEHGIPTAQA
HHEEEEEECCCCCEEECCCCHHHHCCEEEECCCHHHHHCCCCCHHHHHHHHHCCCCCHHH
QSFDEAAPAKAYAATRPLPIVVKADGLAAGKGVVVAATHAEAEAAIDQIFAGGIGGNGLL
CCCCCCCCCHHHHCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCE
IEDFLEGEEASFFALCDGTSALPFASAQDHKRVGEGDTGANTGGMGAYSPAPVMDAAMTE
EHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RVIDEIITPTVKGMAARGTPFKGMLFAGLMIGPAGPKLIEYNVRFGDPETQVMLPRLQDD
HHHHHHHCHHHHCCCCCCCCCCHHHHHHEEECCCCCCEEEEEEEECCCCCEEECHHHHHH
LLALLLACAEGHLPQKPLHFADEVALTVVLAAKGYPEAPQKGSEIRGVEKAASLPGVSLT
HHHHHHHHHCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHCCHHHHHCCCCCEEE
HAGTRREGTKLLADGGRVLNVTGLGKDVAEARERAYAAVDLIDWPEGFCRHDIGWRALQR
CCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHEEEEECCCCCHHHHCCCCCHHHCC
N
C
>Mature Secondary Structure
MNVLLIGSGGREHAIAKTLAKSPLLSTLFVAPGNPGMMEIAEPVVLKIENHQAVIDFCRL
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCEEEEECCCHHHHHHHHH
QAIGLVVIGPEVPLVAGLTDPLVAAGIKVFGPSQAAAQLEGSKNFTKQFCLEHGIPTAQA
HHEEEEEECCCCCEEECCCCHHHHCCEEEECCCHHHHHCCCCCHHHHHHHHHCCCCCHHH
QSFDEAAPAKAYAATRPLPIVVKADGLAAGKGVVVAATHAEAEAAIDQIFAGGIGGNGLL
CCCCCCCCCHHHHCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCE
IEDFLEGEEASFFALCDGTSALPFASAQDHKRVGEGDTGANTGGMGAYSPAPVMDAAMTE
EHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RVIDEIITPTVKGMAARGTPFKGMLFAGLMIGPAGPKLIEYNVRFGDPETQVMLPRLQDD
HHHHHHHCHHHHCCCCCCCCCCHHHHHHEEECCCCCCEEEEEEEECCCCCEEECHHHHHH
LLALLLACAEGHLPQKPLHFADEVALTVVLAAKGYPEAPQKGSEIRGVEKAASLPGVSLT
HHHHHHHHHCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHCCHHHHHCCCCCEEE
HAGTRREGTKLLADGGRVLNVTGLGKDVAEARERAYAAVDLIDWPEGFCRHDIGWRALQR
CCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHEEEEECCCCCHHHHCCCCCHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]