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The map label for this gene is nemA [H]

Identifier: 182677897

GI number: 182677897

Start: 1033780

End: 1034877

Strand: Direct

Name: nemA [H]

Synonym: Bind_0905

Alternate gene names: 182677897

Gene position: 1033780-1034877 (Clockwise)

Preceding gene: 182677896

Following gene: 182677899

Centisome position: 24.79

GC content: 58.01

Gene sequence:

>1098_bases
ATGAGTCTAGGTGAAAATCTGTTCGAACCGGGCCGGATCGGTGATCTCGTTCTTCCCAATCGTCTTGTCATGGCGCCGCT
TGGGCGGGCGCGGTCCGATCTTGCAACGCGTGCGCCTTTGGAAAGGGTCGCGACTTATTACGGGCAGCGTGCCAGCGCCG
GATTGATTGTTTCCGAGGCGACACATGTCTCTCCGTTCAGTGTCAGCCGCCCGGGTGGGTCGGCGATTCATACACAGGCG
CAGATCGCGGGCTGGAAACAGGTCACCAAAACTGTGCATCGGCAGGGCGGGCGGATTTTCCAGCAATTGTTTCATCTCGG
CCGCAAGGCGCATGTGGAACGCTTGCCAGATAATCGGATACCGATTGCGCCTTCCGCCATTGCGGCCATTGGTGACGTGC
CCGTAGCGGGGGGCCTGGCGCCTTTTCCTGTTCCGCGTGCATTGGAAACGGGAGAGATTCAAGGGATTATTGATGAATTC
CACTTGGCGGCAGTCAATTCCCGCGCCGCCGATTTCGACGGCGTCGAGATCCATGCGGCCAATGGTTATCTGATCGATCA
ATTCCTGCGCGATCGATCCAATCGGCGACAGGATCTCTATGGGGGTTCCATCGAGAACCGCGCGCGCTTTCTGCTTGCGA
TCGTCGATGCGGCGGTGAATGTTTTTGGAGCCAGCCGGGTCGGCGTGAGAATTTCTCCGCATTTCACGCAAGATGGAATT
GACGATTCAAATCCTGCCTCGCTCTATGGTTATGTCGCGCAAGAACTCGATCGCCGCAGGATCGCCTATCTGCATGTGAT
CGAGCCGGATACGATCGCGCCGCCGGAGCGGATCGCACCCCATTTACGCACGCATTATTCCGGTGCCTTGATCCTGTGCG
GCGAGTTTACGCATGAGAGTGGTGAGCGCGCGTTAACCGAAAGACGCGCGGATTTCATCGCCTTTGGCCGGTTGTACATC
GCTAATCCAGATCTCGTCGCCCGTTTTCGTTTGCAGGCGCCGCTCAATGCGCCGGACCCCGCGACTTTCTATAGTGGCGG
CGATGAAGGTTATATTGATTATCCGAGCCTGACGCCGGCGCTCGCCGAAGTGAGCTGA

Upstream 100 bases:

>100_bases
GCCTATGATCGCAGCTTCGTAGTGGGTGAGGCTGGCGCGTCGCGCAGTCCTTGATCTGTATGCATGAAGGACATGTTCAA
AACAGAGAGGAGGATGGTGC

Downstream 100 bases:

>100_bases
CTCTATCAACTGCGGCGGGGCGATCCAGACGCCCCGCTCTGTTCTTGTCTCTCCAGCTCTCCCGTGGAAGTCGTGGAGTT
GCTCTGGTGAAATTTAAGCA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSLGENLFEPGRIGDLVLPNRLVMAPLGRARSDLATRAPLERVATYYGQRASAGLIVSEATHVSPFSVSRPGGSAIHTQA
QIAGWKQVTKTVHRQGGRIFQQLFHLGRKAHVERLPDNRIPIAPSAIAAIGDVPVAGGLAPFPVPRALETGEIQGIIDEF
HLAAVNSRAADFDGVEIHAANGYLIDQFLRDRSNRRQDLYGGSIENRARFLLAIVDAAVNVFGASRVGVRISPHFTQDGI
DDSNPASLYGYVAQELDRRRIAYLHVIEPDTIAPPERIAPHLRTHYSGALILCGEFTHESGERALTERRADFIAFGRLYI
ANPDLVARFRLQAPLNAPDPATFYSGGDEGYIDYPSLTPALAEVS

Sequences:

>Translated_365_residues
MSLGENLFEPGRIGDLVLPNRLVMAPLGRARSDLATRAPLERVATYYGQRASAGLIVSEATHVSPFSVSRPGGSAIHTQA
QIAGWKQVTKTVHRQGGRIFQQLFHLGRKAHVERLPDNRIPIAPSAIAAIGDVPVAGGLAPFPVPRALETGEIQGIIDEF
HLAAVNSRAADFDGVEIHAANGYLIDQFLRDRSNRRQDLYGGSIENRARFLLAIVDAAVNVFGASRVGVRISPHFTQDGI
DDSNPASLYGYVAQELDRRRIAYLHVIEPDTIAPPERIAPHLRTHYSGALILCGEFTHESGERALTERRADFIAFGRLYI
ANPDLVARFRLQAPLNAPDPATFYSGGDEGYIDYPSLTPALAEVS
>Mature_364_residues
SLGENLFEPGRIGDLVLPNRLVMAPLGRARSDLATRAPLERVATYYGQRASAGLIVSEATHVSPFSVSRPGGSAIHTQAQ
IAGWKQVTKTVHRQGGRIFQQLFHLGRKAHVERLPDNRIPIAPSAIAAIGDVPVAGGLAPFPVPRALETGEIQGIIDEFH
LAAVNSRAADFDGVEIHAANGYLIDQFLRDRSNRRQDLYGGSIENRARFLLAIVDAAVNVFGASRVGVRISPHFTQDGID
DSNPASLYGYVAQELDRRRIAYLHVIEPDTIAPPERIAPHLRTHYSGALILCGEFTHESGERALTERRADFIAFGRLYIA
NPDLVARFRLQAPLNAPDPATFYSGGDEGYIDYPSLTPALAEVS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=42.8176795580111, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1789463, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=264, Percent_Identity=29.9242424242424, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=185, Percent_Identity=31.8918918918919, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=242, Percent_Identity=28.099173553719, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=171, Percent_Identity=35.0877192982456, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=231, Percent_Identity=28.1385281385281, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=229, Percent_Identity=28.82096069869, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=35.3562005277045, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=37.1428571428571, Blast_Score=199, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39738; Mature: 39607

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGENLFEPGRIGDLVLPNRLVMAPLGRARSDLATRAPLERVATYYGQRASAGLIVSEA
CCCCCCCCCCCCCCCEECCCCEEECCCCCHHHHHHHHCHHHHHHHHHCCCCCCCEEEECC
THVSPFSVSRPGGSAIHTQAQIAGWKQVTKTVHRQGGRIFQQLFHLGRKAHVERLPDNRI
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCC
PIAPSAIAAIGDVPVAGGLAPFPVPRALETGEIQGIIDEFHLAAVNSRAADFDGVEIHAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEC
NGYLIDQFLRDRSNRRQDLYGGSIENRARFLLAIVDAAVNVFGASRVGVRISPHFTQDGI
CCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
DDSNPASLYGYVAQELDRRRIAYLHVIEPDTIAPPERIAPHLRTHYSGALILCGEFTHES
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
GERALTERRADFIAFGRLYIANPDLVARFRLQAPLNAPDPATFYSGGDEGYIDYPSLTPA
HHHHHHHHHHHHEEECEEEEECCCCEEEEEEECCCCCCCCCCEECCCCCCEEECCCCCCH
LAEVS
HHCCC
>Mature Secondary Structure 
SLGENLFEPGRIGDLVLPNRLVMAPLGRARSDLATRAPLERVATYYGQRASAGLIVSEA
CCCCCCCCCCCCCCEECCCCEEECCCCCHHHHHHHHCHHHHHHHHHCCCCCCCEEEECC
THVSPFSVSRPGGSAIHTQAQIAGWKQVTKTVHRQGGRIFQQLFHLGRKAHVERLPDNRI
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCC
PIAPSAIAAIGDVPVAGGLAPFPVPRALETGEIQGIIDEFHLAAVNSRAADFDGVEIHAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEC
NGYLIDQFLRDRSNRRQDLYGGSIENRARFLLAIVDAAVNVFGASRVGVRISPHFTQDGI
CCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
DDSNPASLYGYVAQELDRRRIAYLHVIEPDTIAPPERIAPHLRTHYSGALILCGEFTHES
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
GERALTERRADFIAFGRLYIANPDLVARFRLQAPLNAPDPATFYSGGDEGYIDYPSLTPA
HHHHHHHHHHHHEEECEEEEECCCCEEEEEEECCCCCCCCCCEECCCCCCEEECCCCCCH
LAEVS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]