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The map label for this gene is wrbA3 [H]

Identifier: 182677761

GI number: 182677761

Start: 862471

End: 863097

Strand: Direct

Name: wrbA3 [H]

Synonym: Bind_0768

Alternate gene names: 182677761

Gene position: 862471-863097 (Clockwise)

Preceding gene: 182677753

Following gene: 182677764

Centisome position: 20.68

GC content: 62.36

Gene sequence:

>627_bases
ATGGCGGAAACCAATGTCCTGATCGCCTTTTATTCGCGCAGTGGCGTGACCGAGGCTCTGGCGAAAGCCATAGCCGAGGG
AGCGGAGGCTTCGGGCGCGGTTGTTCGCCTGCGGCGGGCGCGCGAACTCGTATCCCCTGAAGTCATGGCGCTCGCACCCG
GCTGGAAGGAAGCCGCCGAGGCCATGAATGCGCGATATGAGGCTCCGACGAGTGCCGATGCCGAATGGGCTGATGCCTTG
GTGCTGGGGACGCCGACACGGTTCGGCGCGATGTCCTCGGAGCTCAAGGCCTATATTGACGGGCTGGGCGGGCTTTGGGT
GAAAGGGAAGCTGGTTGGCAAGGCGGGCTCGGTCTTCTGTTCCACTTCGACCCCGCATGGCGGCAATGAATCGACCTTGC
TGTCCATGTATAATCCGCTGGCGCATCTCGGCTTCATTATCGTTCCGACAGGCTATGCCGATCCCAGCCTCTACAAGGCG
GGCACGCCTTATGGCTCTAGCGCCATCTCCTTTAGCAAGGCGGAGCCACCGACGGCGGATGATCTCGAAGTGGCCCGTTT
TCAGGGCCGCCGAGTCACGGAAGTCGCGGCGGCCTTGAAGGCCTTTGCTTTGGCCAAGGCGGCATAA

Upstream 100 bases:

>100_bases
GCAACCGACCTTGGGCTTGAACGCAGGAACCAAGCTGTTTTGCGGGCGGTTTCTCCTCGCAATTCATGGCCTGCAAATTG
TCGCAGGGGAGATCACTCTC

Downstream 100 bases:

>100_bases
TCATCAACATCATCAACCTGGTGCTCAAAGGTCTTCCAGCCCCAAAGCGGCGGTGAATTCCCGCCATTCGCGCTCGCCGA
GCCCGCTCGATGTTTGCGTA

Product: flavoprotein WrbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MAETNVLIAFYSRSGVTEALAKAIAEGAEASGAVVRLRRARELVSPEVMALAPGWKEAAEAMNARYEAPTSADAEWADAL
VLGTPTRFGAMSSELKAYIDGLGGLWVKGKLVGKAGSVFCSTSTPHGGNESTLLSMYNPLAHLGFIIVPTGYADPSLYKA
GTPYGSSAISFSKAEPPTADDLEVARFQGRRVTEVAAALKAFALAKAA

Sequences:

>Translated_208_residues
MAETNVLIAFYSRSGVTEALAKAIAEGAEASGAVVRLRRARELVSPEVMALAPGWKEAAEAMNARYEAPTSADAEWADAL
VLGTPTRFGAMSSELKAYIDGLGGLWVKGKLVGKAGSVFCSTSTPHGGNESTLLSMYNPLAHLGFIIVPTGYADPSLYKA
GTPYGSSAISFSKAEPPTADDLEVARFQGRRVTEVAAALKAFALAKAA
>Mature_207_residues
AETNVLIAFYSRSGVTEALAKAIAEGAEASGAVVRLRRARELVSPEVMALAPGWKEAAEAMNARYEAPTSADAEWADALV
LGTPTRFGAMSSELKAYIDGLGGLWVKGKLVGKAGSVFCSTSTPHGGNESTLLSMYNPLAHLGFIIVPTGYADPSLYKAG
TPYGSSAISFSKAEPPTADDLEVARFQGRRVTEVAAALKAFALAKAA

Specific function: Seems To Enhance The Formation And/Or Stability Of Noncovalent Complexes Between The Trp Repressor Protein And Operator-Bearing DNA. However, Wrba Does Not Specifically Influence The Affinity Or Mode Of Binding Of Trpr To Its Operator. It Seems Possible T

COG id: COG0655

COG function: function code R; Multimeric flavodoxin WrbA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787239, Length=205, Percent_Identity=40, Blast_Score=135, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320235, Length=202, Percent_Identity=35.1485148514851, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319849, Length=210, Percent_Identity=32.8571428571429, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319526, Length=205, Percent_Identity=28.2926829268293, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008254
- InterPro:   IPR010089
- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: NA

Molecular weight: Translated: 21673; Mature: 21542

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETNVLIAFYSRSGVTEALAKAIAEGAEASGAVVRLRRARELVSPEVMALAPGWKEAAE
CCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHH
AMNARYEAPTSADAEWADALVLGTPTRFGAMSSELKAYIDGLGGLWVKGKLVGKAGSVFC
HHHCCCCCCCCCCCHHHCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
STSTPHGGNESTLLSMYNPLAHLGFIIVPTGYADPSLYKAGTPYGSSAISFSKAEPPTAD
ECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCCCCCCCEECCCCCCCCCC
DLEVARFQGRRVTEVAAALKAFALAKAA
CHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AETNVLIAFYSRSGVTEALAKAIAEGAEASGAVVRLRRARELVSPEVMALAPGWKEAAE
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHH
AMNARYEAPTSADAEWADALVLGTPTRFGAMSSELKAYIDGLGGLWVKGKLVGKAGSVFC
HHHCCCCCCCCCCCHHHCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
STSTPHGGNESTLLSMYNPLAHLGFIIVPTGYADPSLYKAGTPYGSSAISFSKAEPPTAD
ECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCCCCCCCEECCCCCCCCCC
DLEVARFQGRRVTEVAAALKAFALAKAA
CHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]