Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 182677727

GI number: 182677727

Start: 824069

End: 824443

Strand: Direct

Name: gcvH

Synonym: Bind_0734

Alternate gene names: 182677727

Gene position: 824069-824443 (Clockwise)

Preceding gene: 182677726

Following gene: 182677728

Centisome position: 19.76

GC content: 60.8

Gene sequence:

>375_bases
ATGTCGATCACCCGCTACACCAAGGACCATGAATATATCCGCGTCGAGGGAGAGACCGGCACGGTCGGCATTACCGATTA
CGCCCAGTCGCAATTGGGCGATGTCGTCTTCGTGGAACTGCCGGCCATCGGCAAGGCCCTGACCAAGGGCGGTGAGGCTG
CGGTCGTTGAAAGCGTGAAGGCCGCGAGCGAGATCTATGCCCCGGTGTCGGGCGAGGTCGTGGCGGTCAACGAGGCTTTG
GCCGAGGCACCGGGCACCGTCAATGAAGATGCAGCCGGCAAAGGCTGGTTCCTGCAAATCAAGCTCGCCGATGCCAAGGA
ACTCGACGGCCTGATGGACGAGGCCGGCTATCAGGATTTCCTGAAAACGCTTTGA

Upstream 100 bases:

>100_bases
CCAATGGGACTGCCTTACAGGTGATCGTCCGAGGAAAACCACTCGCCGCGACGATTACCTCCATGCCTTTTGTTCCGAAT
CATTATTACAGGGGCGCCTG

Downstream 100 bases:

>100_bases
GCCGATCAGATGGTTTCGATCCGATAGGAGAAAGAAATGCGCTATCTGCCTTTGACGCCGGAGGATCGGACCGAAATGCT
GGCGCGCGTCGGCGTCCCCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MSITRYTKDHEYIRVEGETGTVGITDYAQSQLGDVVFVELPAIGKALTKGGEAAVVESVKAASEIYAPVSGEVVAVNEAL
AEAPGTVNEDAAGKGWFLQIKLADAKELDGLMDEAGYQDFLKTL

Sequences:

>Translated_124_residues
MSITRYTKDHEYIRVEGETGTVGITDYAQSQLGDVVFVELPAIGKALTKGGEAAVVESVKAASEIYAPVSGEVVAVNEAL
AEAPGTVNEDAAGKGWFLQIKLADAKELDGLMDEAGYQDFLKTL
>Mature_123_residues
SITRYTKDHEYIRVEGETGTVGITDYAQSQLGDVVFVELPAIGKALTKGGEAAVVESVKAASEIYAPVSGEVVAVNEALA
EAPGTVNEDAAGKGWFLQIKLADAKELDGLMDEAGYQDFLKTL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=124, Percent_Identity=46.7741935483871, Blast_Score=133, Evalue=4e-32,
Organism=Homo sapiens, GI49574537, Length=124, Percent_Identity=46.7741935483871, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=51.2605042016807, Blast_Score=127, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=51.2820512820513, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=48.695652173913, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=44.6280991735537, Blast_Score=105, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=45.8333333333333, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BEII9 (B2IGK2)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831873.1
- GeneID:   6198582
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0734
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13124; Mature: 12993

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITRYTKDHEYIRVEGETGTVGITDYAQSQLGDVVFVELPAIGKALTKGGEAAVVESVK
CCCCCCCCCCCEEEEECCCCEEECHHHHHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHH
AASEIYAPVSGEVVAVNEALAEAPGTVNEDAAGKGWFLQIKLADAKELDGLMDEAGYQDF
HHHHHCCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHH
LKTL
HHCC
>Mature Secondary Structure 
SITRYTKDHEYIRVEGETGTVGITDYAQSQLGDVVFVELPAIGKALTKGGEAAVVESVK
CCCCCCCCCCEEEEECCCCEEECHHHHHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHH
AASEIYAPVSGEVVAVNEALAEAPGTVNEDAAGKGWFLQIKLADAKELDGLMDEAGYQDF
HHHHHCCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHH
LKTL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA