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The map label for this gene is ddl

Identifier: 182677688

GI number: 182677688

Start: 777866

End: 778789

Strand: Direct

Name: ddl

Synonym: Bind_0695

Alternate gene names: 182677688

Gene position: 777866-778789 (Clockwise)

Preceding gene: 182677687

Following gene: 182677689

Centisome position: 18.65

GC content: 59.09

Gene sequence:

>924_bases
GTGGCGAAACATGTGGCCGTGCTGATGGGCGGTCTTTCGGCCGAGCGCGAGGTCTCGCTGTGCTCGGGGGCGGCTTGTGC
TGAGGCTCTGCGCGGCGAGGGGTTCGAGGTCACGGAAGTGGATGTCGATCGCCAGATCGGCGAAAGGCTCGCGGCCTTGC
GGCCTGATGTCGCTTTCAATGCTTTGCATGGCCGATTCGGCGAGGATGGCATCATCCAGGGCGTTCTGGAAATGCTGGCC
ATTCCCTATACGCATTCGGGCGTTCTCGCCTCGGCGCTCGCCATGCGCAAGGACCGTGCGCGCGACGTTCTGGCCGCTGC
GGGTGTCCCGATTGCCAAGGGCGTCACGGTTGACCGGCTGGAGGCGGCGCAACGCCATATTTTGCCACCGCCTTACGTGA
TCAAGCCGCTCGGCGAAGGCTCTTCCTTCGGCGTGTTCATCGTGCGGGAGGATCAGGCCTATCCGCCACAAGAGTTAACG
CGTTCCGACTGGGCTTTCGGTAACCGTGTTCTGGTTGAATCCTATATAGGAGGGCGCGAGCTGACCTGTGCCGTCATCGG
CGAAAAAGCTCATGATGTCATAGAGATAAAGGCCGTTGGCGGCGGCTGGTACGATTATGACGCCAAATATCGAAAAGGTG
GATCTATCCACATCCTTCCTGCCGAACTTAAACGAAATATTTACCAAAATGTTCAACTCTTATCGCTCAAGGCACATAAG
GCCCTCGGCTGCCGTGGCGTGAGCCGGACTGATTTCCGTTACGACGACCGTCCCGAAGGGACGGGCGAATTGATCGTCCT
GGAAGTCAACACTCAGCCCGGCATGACGGAGACCTCCTTGCTGCCAGAAATTGCGGCCTATGCCGGTCTCTCGTTTGGTG
AGCTTGTTCGATGGATGGTGGACGACGCCTCCTGCGATCGTTGA

Upstream 100 bases:

>100_bases
GCTGGCCGAAACGGGTGTGGAACTCGAATGGGAAATCCTGCGGGTTGGTCGGCCGCCCGTTGCTCATAAAACTTCACGCT
GACTGAAGGAGAAGCGGATC

Downstream 100 bases:

>100_bases
TTTTTTCGAAAGCGGGGCTTGGCCCCGCGGCCTTCGCCTATGCGGGGACATCGGCCAGCGCGAGGAGCCAGTGGGTTTCC
TTTGCACCGTCCGTCCGTCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAKHVAVLMGGLSAEREVSLCSGAACAEALRGEGFEVTEVDVDRQIGERLAALRPDVAFNALHGRFGEDGIIQGVLEMLA
IPYTHSGVLASALAMRKDRARDVLAAAGVPIAKGVTVDRLEAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELT
RSDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHK
ALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMVDDASCDR

Sequences:

>Translated_307_residues
MAKHVAVLMGGLSAEREVSLCSGAACAEALRGEGFEVTEVDVDRQIGERLAALRPDVAFNALHGRFGEDGIIQGVLEMLA
IPYTHSGVLASALAMRKDRARDVLAAAGVPIAKGVTVDRLEAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELT
RSDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHK
ALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMVDDASCDR
>Mature_306_residues
AKHVAVLMGGLSAEREVSLCSGAACAEALRGEGFEVTEVDVDRQIGERLAALRPDVAFNALHGRFGEDGIIQGVLEMLAI
PYTHSGVLASALAMRKDRARDVLAAAGVPIAKGVTVDRLEAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTR
SDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHKA
LGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMVDDASCDR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=40.9836065573771, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=30.5949008498584, Blast_Score=140, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BEII9 (B2IGG3)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831834.1
- ProteinModelPortal:   B2IGG3
- GeneID:   6198336
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0695
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33305; Mature: 33174

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHVAVLMGGLSAEREVSLCSGAACAEALRGEGFEVTEVDVDRQIGERLAALRPDVAFN
CCCCEEEECCCCCCCCHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
ALHGRFGEDGIIQGVLEMLAIPYTHSGVLASALAMRKDRARDVLAAAGVPIAKGVTVDRL
HHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHH
EAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTRSDWAFGNRVLVESYIGGRE
HHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEECCCCCE
LTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHK
EEEEEECCCCCCEEEEEEECCCEECCCCCCCCCCCEEEEHHHHHHHHHHCEEEEEEEHHH
ALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMV
HHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
DDASCDR
CCCCCCC
>Mature Secondary Structure 
AKHVAVLMGGLSAEREVSLCSGAACAEALRGEGFEVTEVDVDRQIGERLAALRPDVAFN
CCCEEEECCCCCCCCHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
ALHGRFGEDGIIQGVLEMLAIPYTHSGVLASALAMRKDRARDVLAAAGVPIAKGVTVDRL
HHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHH
EAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTRSDWAFGNRVLVESYIGGRE
HHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEECCCCCE
LTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHK
EEEEEECCCCCCEEEEEEECCCEECCCCCCCCCCCEEEEHHHHHHHHHHCEEEEEEEHHH
ALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMV
HHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
DDASCDR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA