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The map label for this gene is mraW

Identifier: 182677677

GI number: 182677677

Start: 764320

End: 765387

Strand: Direct

Name: mraW

Synonym: Bind_0684

Alternate gene names: 182677677

Gene position: 764320-765387 (Clockwise)

Preceding gene: 182677676

Following gene: 182677678

Centisome position: 18.33

GC content: 63.95

Gene sequence:

>1068_bases
ATGAAATCGGGCCGCGGCGATGAGTCTCCTCTCGCCGCAGGCGGACCGGCCCGACACGTTCCCGTGCTTCGTGAGGAAGT
CGTCGCAGCCCTCGATTGCAAAAAAGGCGGTTTCTATCTCGATGCCACTTTCGGGGCAGGGGGATATACGCGCGCTTTGC
TCGCCGAGCCTGAAACGCACGTGCTGGCGATTGATCGCGATCCCGAAGCGATTGCGGCAGGCCGGGCTCTGGAGGCGGAA
AGCGCCGGCAGGCTTATCCTCGCGCATGGCCGTTTCTCGACGCTCGCCGATATCGCTGCCGCCGAGGGCATTCCTGCATT
CGACGGCATCGTGTTCGATATCGGCGTTTCCTCGATGCAGCTCGATCAGCCGGAACGGGGGTTTTCCTTTCGTGGCGAGG
GGCCGCTCGACATGCGGATGGAAAGCCGTGGGCCAAGCGCTGCCGATCTCGTCAACACGGCCGATGAGCAAAGGCTCGCG
GATATATTCTATTATTTTGGTGAGGAGCGGGCGTCCCGCCGCATCGCCCGGGCGATCGTCACCGACCGCGCGCATACGCC
CTTCACCACGACACGCCAGCTTGCCGAACTGATCGCCAGGGTCAATCCGGGTAAGCCCGGAGACATCCATCCGGCGACCC
GCGTCTTCCAGGCCTTGCGCATTGCCGTCAATGAAGAGCTTCTCGAACTCGTGCGCGCCTTGGCCGCCGCCGAAGCTCTG
TTGCGTGAGGGTGGCAGGCTCGTCGTGGTGACTTTCCATTCTTTGGAGGATCGGATCGTCAAACAATTTCTGGCTGCCCG
TTCCGGCCGTGGCCAGGCCGTATCACGGCCGCTGCCGGGTGAGCCCGCGCTCGCGCCGCCCACCTTCATTCTGCCCGGCA
AGCAGCCGGTCCTGCCTTCACCGGAAGAAATCGCCGTCAATCCGCGTGCCCGTTCGGCGAAACTGCGGTTCGGCCTGCGC
ACGGCCGCGCCGGCGCGGGGCCTTGAGTCTGGTCTGTTGCCGCTCGCGACTCTGCCGGAAACCAGCCATCCAAAATCCGC
TTCCCATTCCAAATCAAGGAGGCGCTGA

Upstream 100 bases:

>100_bases
TGGAAGAGGCCAGAAACCGGGTGCGCGATCTCCGCAAGCAGCTCAGTTCCAGACCCGTGGCGCCGGATGCGCAGCCGCCA
CGACCACATGGAGCACGGGA

Downstream 100 bases:

>100_bases
ATGATCCGTGTCCTCAATCTTTTGACGGTTCTGGCTCTGCTCGGTTCCGCCATTTACGCCTATTCGATCAAATATGAGAC
CGTGCTTCGGGCTGAAACGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKSGRGDESPLAAGGPARHVPVLREEVVAALDCKKGGFYLDATFGAGGYTRALLAEPETHVLAIDRDPEAIAAGRALEAE
SAGRLILAHGRFSTLADIAAAEGIPAFDGIVFDIGVSSMQLDQPERGFSFRGEGPLDMRMESRGPSAADLVNTADEQRLA
DIFYYFGEERASRRIARAIVTDRAHTPFTTTRQLAELIARVNPGKPGDIHPATRVFQALRIAVNEELLELVRALAAAEAL
LREGGRLVVVTFHSLEDRIVKQFLAARSGRGQAVSRPLPGEPALAPPTFILPGKQPVLPSPEEIAVNPRARSAKLRFGLR
TAAPARGLESGLLPLATLPETSHPKSASHSKSRRR

Sequences:

>Translated_355_residues
MKSGRGDESPLAAGGPARHVPVLREEVVAALDCKKGGFYLDATFGAGGYTRALLAEPETHVLAIDRDPEAIAAGRALEAE
SAGRLILAHGRFSTLADIAAAEGIPAFDGIVFDIGVSSMQLDQPERGFSFRGEGPLDMRMESRGPSAADLVNTADEQRLA
DIFYYFGEERASRRIARAIVTDRAHTPFTTTRQLAELIARVNPGKPGDIHPATRVFQALRIAVNEELLELVRALAAAEAL
LREGGRLVVVTFHSLEDRIVKQFLAARSGRGQAVSRPLPGEPALAPPTFILPGKQPVLPSPEEIAVNPRARSAKLRFGLR
TAAPARGLESGLLPLATLPETSHPKSASHSKSRRR
>Mature_355_residues
MKSGRGDESPLAAGGPARHVPVLREEVVAALDCKKGGFYLDATFGAGGYTRALLAEPETHVLAIDRDPEAIAAGRALEAE
SAGRLILAHGRFSTLADIAAAEGIPAFDGIVFDIGVSSMQLDQPERGFSFRGEGPLDMRMESRGPSAADLVNTADEQRLA
DIFYYFGEERASRRIARAIVTDRAHTPFTTTRQLAELIARVNPGKPGDIHPATRVFQALRIAVNEELLELVRALAAAEAL
LREGGRLVVVTFHSLEDRIVKQFLAARSGRGQAVSRPLPGEPALAPPTFILPGKQPVLPSPEEIAVNPRARSAKLRFGLR
TAAPARGLESGLLPLATLPETSHPKSASHSKSRRR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=42.433234421365, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=44.5026178010471, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=46.5189873417722, Blast_Score=242, Evalue=3e-65,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=41.0876132930514, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=41.0876132930514, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BEII9 (B2IGF2)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831823.1
- ProteinModelPortal:   B2IGF2
- GeneID:   6201760
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0684
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 38043; Mature: 38043

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 92-92 BINDING 113-113 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSGRGDESPLAAGGPARHVPVLREEVVAALDCKKGGFYLDATFGAGGYTRALLAEPETH
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEECCCCE
VLAIDRDPEAIAAGRALEAESAGRLILAHGRFSTLADIAAAEGIPAFDGIVFDIGVSSMQ
EEEECCCCHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHCCEEEECCCCCEE
LDQPERGFSFRGEGPLDMRMESRGPSAADLVNTADEQRLADIFYYFGEERASRRIARAIV
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDRAHTPFTTTRQLAELIARVNPGKPGDIHPATRVFQALRIAVNEELLELVRALAAAEAL
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREGGRLVVVTFHSLEDRIVKQFLAARSGRGQAVSRPLPGEPALAPPTFILPGKQPVLPS
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCC
PEEIAVNPRARSAKLRFGLRTAAPARGLESGLLPLATLPETSHPKSASHSKSRRR
CHHEEECCCCCCCEEEECCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MKSGRGDESPLAAGGPARHVPVLREEVVAALDCKKGGFYLDATFGAGGYTRALLAEPETH
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEECCCCE
VLAIDRDPEAIAAGRALEAESAGRLILAHGRFSTLADIAAAEGIPAFDGIVFDIGVSSMQ
EEEECCCCHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHCCEEEECCCCCEE
LDQPERGFSFRGEGPLDMRMESRGPSAADLVNTADEQRLADIFYYFGEERASRRIARAIV
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDRAHTPFTTTRQLAELIARVNPGKPGDIHPATRVFQALRIAVNEELLELVRALAAAEAL
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREGGRLVVVTFHSLEDRIVKQFLAARSGRGQAVSRPLPGEPALAPPTFILPGKQPVLPS
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCC
PEEIAVNPRARSAKLRFGLRTAAPARGLESGLLPLATLPETSHPKSASHSKSRRR
CHHEEECCCCCCCEEEECCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA