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The map label for this gene is ybjS [C]

Identifier: 182677030

GI number: 182677030

Start: 30519

End: 31511

Strand: Direct

Name: ybjS [C]

Synonym: Bind_0028

Alternate gene names: 182677030

Gene position: 30519-31511 (Clockwise)

Preceding gene: 182677029

Following gene: 182677031

Centisome position: 0.73

GC content: 59.42

Gene sequence:

>993_bases
ATGAAAATTCTCGTCACAGGAGGCACTGGCCTTCTCGGCGGCCGCTTGATCCCGAGACTCGTGAAGGACGGTTACCAAAT
ATTCGCCCTCACACGTTCCGCATCGTCTCACGACAAACTAAAGGCATTGGGTGCATCTCCAGTCGACGCTGATCTGGAAA
GCAGCGCGCCTCTGGCATTGCCAGCGATCGATGCCGTTGTGCACGCAGCCGCCCTGTTTCGCTTTGCGGGGCCACGCGAG
CCCTTTTTTCGCACCAATGTCGACGGCACCGTCGCGCTTCTGAAGGCCACCGAGAAAGCCGGGGCGCAGACCTTCGTCTA
TATCAGTGCGGCGGGAATCATCATGGATGATGCCGGCACGCCTATCCGCGGTGCCGATGAAAGCGCCCCCATATTCCCGA
ACCACTTCTCTGCCTATCTGGCGAGCAAGGCACGGGCCGAGCCGTTGGTGCTGGCGGCCAACAAGCCTGGCTTTCGCACG
ATCGCGCTTCGCCCACCGGCAATATGGGGGCCTGGTGATTCCTTCAGCCGTGAGCTTCCCCGTGTAATAAAGTCCGGGCA
GTTCGCCTTCATCGATCGCGGCGACTACGCCTTTGCAACCTGCCATGTGGACAATGTGGTTGAAGCCGTACAATGCGCCC
TTGAGTGCGGAGAGGGCGGTCGTGCCTTCTTCATCAACGATCAGGAAAGGCTGACCTTCCGGCAGTTCATCGCGTCGCTC
GCGAGCTTGCAAGGTTTATCGATCGATAAGCTGCGGTCGATGCCTTACTGGCTCGCATCTGCTGTTGGCCGAATGCTGGA
CGCTATCTGGGCCATGACGCGGAGAGAAGGCAATCCACCGATCTCGCGCTCGATGATACGCATGATCGGGCGGGAGTTCA
GCATCAACGATGCTGCTGCACGCCGTGAGTTGGGCTATGTCGGAAGGACTTCGCGCGCTGATGGCTTGCGAAGCTATGGG
ACTCCCCTTCGATCGGCGGATCGATTTCCGTGA

Upstream 100 bases:

>100_bases
AGCTCCGCAAGACGGCGCCGCCCGCTTCTGCGGTCGTCGAAAACATCCGCGAGAGCGCCATGCAAGCCATCCTGAACATT
GAATCAAAAGGTCAATCGCA

Downstream 100 bases:

>100_bases
GCCGTACCCTGAACTGCGCCCTCGAGAGCCGCATGATCGCGGGCTTCATGCCGAAAGATAACCGACGGATGAATCAATCC
CGCCTCTCGTCATAGCGGCG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKILVTGGTGLLGGRLIPRLVKDGYQIFALTRSASSHDKLKALGASPVDADLESSAPLALPAIDAVVHAAALFRFAGPRE
PFFRTNVDGTVALLKATEKAGAQTFVYISAAGIIMDDAGTPIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRT
IALRPPAIWGPGDSFSRELPRVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRAFFINDQERLTFRQFIASL
ASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPISRSMIRMIGREFSINDAAARRELGYVGRTSRADGLRSYG
TPLRSADRFP

Sequences:

>Translated_330_residues
MKILVTGGTGLLGGRLIPRLVKDGYQIFALTRSASSHDKLKALGASPVDADLESSAPLALPAIDAVVHAAALFRFAGPRE
PFFRTNVDGTVALLKATEKAGAQTFVYISAAGIIMDDAGTPIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRT
IALRPPAIWGPGDSFSRELPRVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRAFFINDQERLTFRQFIASL
ASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPISRSMIRMIGREFSINDAAARRELGYVGRTSRADGLRSYG
TPLRSADRFP
>Mature_330_residues
MKILVTGGTGLLGGRLIPRLVKDGYQIFALTRSASSHDKLKALGASPVDADLESSAPLALPAIDAVVHAAALFRFAGPRE
PFFRTNVDGTVALLKATEKAGAQTFVYISAAGIIMDDAGTPIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRT
IALRPPAIWGPGDSFSRELPRVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRAFFINDQERLTFRQFIASL
ASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPISRSMIRMIGREFSINDAAARRELGYVGRTSRADGLRSYG
TPLRSADRFP

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI310132178, Length=357, Percent_Identity=30.5322128851541, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310113012, Length=357, Percent_Identity=30.5322128851541, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239745448, Length=357, Percent_Identity=30.5322128851541, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI193211614, Length=273, Percent_Identity=29.3040293040293, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI8393516, Length=273, Percent_Identity=29.3040293040293, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116268111, Length=338, Percent_Identity=27.810650887574, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4504507, Length=344, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI260763931, Length=349, Percent_Identity=26.3610315186246, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4504509, Length=349, Percent_Identity=26.3610315186246, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI87081792, Length=343, Percent_Identity=26.530612244898, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17570557, Length=340, Percent_Identity=25.8823529411765, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509709, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321437, Length=339, Percent_Identity=27.4336283185841, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 35620; Mature: 35620

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGTGLLGGRLIPRLVKDGYQIFALTRSASSHDKLKALGASPVDADLESSAPLAL
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCEEH
PAIDAVVHAAALFRFAGPREPFFRTNVDGTVALLKATEKAGAQTFVYISAAGIIMDDAGT
HHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEHHHCCCEEEEEEEECCEEEECCCC
PIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRTIALRPPAIWGPGDSFSRELP
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHH
RVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRAFFINDQERLTFRQFIASL
HHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
ASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPISRSMIRMIGREFSINDAAA
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHH
RRELGYVGRTSRADGLRSYGTPLRSADRFP
HHHHCCCCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGTGLLGGRLIPRLVKDGYQIFALTRSASSHDKLKALGASPVDADLESSAPLAL
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCEEH
PAIDAVVHAAALFRFAGPREPFFRTNVDGTVALLKATEKAGAQTFVYISAAGIIMDDAGT
HHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEHHHCCCEEEEEEEECCEEEECCCC
PIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRTIALRPPAIWGPGDSFSRELP
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHH
RVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRAFFINDQERLTFRQFIASL
HHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
ASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPISRSMIRMIGREFSINDAAA
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHH
RRELGYVGRTSRADGLRSYGTPLRSADRFP
HHHHCCCCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]