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The map label for this gene is puuB [H]

Identifier: 17548124

GI number: 17548124

Start: 3683472

End: 3684779

Strand: Reverse

Name: puuB [H]

Synonym: RSc3407

Alternate gene names: 17548124

Gene position: 3684779-3683472 (Counterclockwise)

Preceding gene: 17548125

Following gene: 17548122

Centisome position: 99.15

GC content: 72.78

Gene sequence:

>1308_bases
ATGACCATGCCGTCGCCCGACATCGTGCCCGACGGCGAGCCGCTGCCGCCCTCGCTGTGGGCCGCGACGGCGCCGGCCGC
GCCGCCCACGCCGGCCCTGCGGGAATCGATCGCCACCGATGTGCTGGTCATCGGCGGCGGCTACACGGGGCTATCGACCG
CGCTGCACCTGGCCGAACGCGGCACGGGCGTGACGGTGCTGGAAGCCAACGACCCGGGCTGGGGCGCCTCCGGGCGCAAC
GGCGGGCAGGTCAACCCGACGCTCAAGCACGACCCGGACGAACTGGTGCGCATGCTGGGCGCCGCGCGCGCCGAGCCGCT
GATCGATGCGGTGTCGCGCTCGGCCGATCTGGTGTTTGACCTGATCGGCCGGCACGGCATCGACTGCCAGCCGGTGCGCG
CGGGCTGGCTGCAGCTGTCGTATTCAGACAAGGCGGTGCCGGCCATGCATGCGCGGGCGCACCAGTGGGCGCGGCGCGGG
GTGCGGGTCGAGCTGCTGGACCGGGCCGCGGTGGCGCGCCGCGTGGGCACCGAGGCGTTCGCCGGCGGCTGGCTGGACGG
CCGGGCGGGCGCCATCCAGCCGCTGGCCTATGCGCGCGGCCTGGTGCACGCCGCACAACGAGCGGGCGCCGCCGTGCACG
GCAACACGGCCGTCACCGCCCTGGAGCGGCAGGGCACGGCATGGATCGCATCCACCCGCACCGGCGCGCAGGTCCGTGCC
CAGCACGTCGTCATCGCCACCAACGGCTACACCGGTGCGCTGTGGCCGGGGCTGGCACAGACCGTGCTCAGTGCCAACAG
CTTTATCGTCGCGACGCGGCCGCTGAAGCCGGCGGATGCGCAGGCCATCCTGGCGCGCGGCGAAACGGCGTCCACCTCGC
AACGGCTGCTGCTGTATTTCCGCAAGGACGCGGCCGGGCGCCTGCTGATGGGTGGTCGCGGCTTCTTTGCCGAGCCGCGC
GGTCCGCAGGATTTCGCACACCTCGAACGCTCACTGGAGCTGCTGTTTCCGCAGTTGGGCCGGCTCGACTACGCATACCG
GTGGGCCGGCCGCATCGCCATCACGCGGGACTTCATGCCGCACATCCACGAGCCCGCGCCGGGCGTGACGATGGCGCTGG
GCTGCAACGGACGCGGCATCGCGCTGTGCACCAGTCTGGGCCAGCAGATCGCGGCGCGGCTGGCGGACAGCGGCCATGCG
TTCCCGTACCCGGTGTCGCCCATCGTGCCGATTCCGCTGCACGGCCTGCAGCGCTTCTACATCGCCGCCGGGGTGGCGTG
GTACAGCCTGCTGGACAAGCTTGGCTGA

Upstream 100 bases:

>100_bases
CCGCGCAGGTGCTGGGCGCGCCGCGCCAGCCGCGCACGCAGGACTTCCTGCGCCGCGTGCTGCACAAGCCGGCCGAGCCG
GCCACGGGAGACCTGGCGCT

Downstream 100 bases:

>100_bases
GTGCGCTCAGGCGGGCAGGCGCAGCGCCTCGATGAAGGCGCGCATGTGCCCCAGCACCGCATCGGCGTGCGTCCTGTGCG
GCGTGTGCCCGCAGCCGGGC

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTMPSPDIVPDGEPLPPSLWAATAPAAPPTPALRESIATDVLVIGGGYTGLSTALHLAERGTGVTVLEANDPGWGASGRN
GGQVNPTLKHDPDELVRMLGAARAEPLIDAVSRSADLVFDLIGRHGIDCQPVRAGWLQLSYSDKAVPAMHARAHQWARRG
VRVELLDRAAVARRVGTEAFAGGWLDGRAGAIQPLAYARGLVHAAQRAGAAVHGNTAVTALERQGTAWIASTRTGAQVRA
QHVVIATNGYTGALWPGLAQTVLSANSFIVATRPLKPADAQAILARGETASTSQRLLLYFRKDAAGRLLMGGRGFFAEPR
GPQDFAHLERSLELLFPQLGRLDYAYRWAGRIAITRDFMPHIHEPAPGVTMALGCNGRGIALCTSLGQQIAARLADSGHA
FPYPVSPIVPIPLHGLQRFYIAAGVAWYSLLDKLG

Sequences:

>Translated_435_residues
MTMPSPDIVPDGEPLPPSLWAATAPAAPPTPALRESIATDVLVIGGGYTGLSTALHLAERGTGVTVLEANDPGWGASGRN
GGQVNPTLKHDPDELVRMLGAARAEPLIDAVSRSADLVFDLIGRHGIDCQPVRAGWLQLSYSDKAVPAMHARAHQWARRG
VRVELLDRAAVARRVGTEAFAGGWLDGRAGAIQPLAYARGLVHAAQRAGAAVHGNTAVTALERQGTAWIASTRTGAQVRA
QHVVIATNGYTGALWPGLAQTVLSANSFIVATRPLKPADAQAILARGETASTSQRLLLYFRKDAAGRLLMGGRGFFAEPR
GPQDFAHLERSLELLFPQLGRLDYAYRWAGRIAITRDFMPHIHEPAPGVTMALGCNGRGIALCTSLGQQIAARLADSGHA
FPYPVSPIVPIPLHGLQRFYIAAGVAWYSLLDKLG
>Mature_434_residues
TMPSPDIVPDGEPLPPSLWAATAPAAPPTPALRESIATDVLVIGGGYTGLSTALHLAERGTGVTVLEANDPGWGASGRNG
GQVNPTLKHDPDELVRMLGAARAEPLIDAVSRSADLVFDLIGRHGIDCQPVRAGWLQLSYSDKAVPAMHARAHQWARRGV
RVELLDRAAVARRVGTEAFAGGWLDGRAGAIQPLAYARGLVHAAQRAGAAVHGNTAVTALERQGTAWIASTRTGAQVRAQ
HVVIATNGYTGALWPGLAQTVLSANSFIVATRPLKPADAQAILARGETASTSQRLLLYFRKDAAGRLLMGGRGFFAEPRG
PQDFAHLERSLELLFPQLGRLDYAYRWAGRIAITRDFMPHIHEPAPGVTMALGCNGRGIALCTSLGQQIAARLADSGHAF
PYPVSPIVPIPLHGLQRFYIAAGVAWYSLLDKLG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=431, Percent_Identity=34.1067285382831, Blast_Score=207, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46128; Mature: 45997

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01067 SECE_SEC61G

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPSPDIVPDGEPLPPSLWAATAPAAPPTPALRESIATDVLVIGGGYTGLSTALHLAER
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHC
GTGVTVLEANDPGWGASGRNGGQVNPTLKHDPDELVRMLGAARAEPLIDAVSRSADLVFD
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LIGRHGIDCQPVRAGWLQLSYSDKAVPAMHARAHQWARRGVRVELLDRAAVARRVGTEAF
HHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHH
AGGWLDGRAGAIQPLAYARGLVHAAQRAGAAVHGNTAVTALERQGTAWIASTRTGAQVRA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCEEEEECCCCCEEEE
QHVVIATNGYTGALWPGLAQTVLSANSFIVATRPLKPADAQAILARGETASTSQRLLLYF
EEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCCCCCCCEEEEEE
RKDAAGRLLMGGRGFFAEPRGPQDFAHLERSLELLFPQLGRLDYAYRWAGRIAITRDFMP
ECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHC
HIHEPAPGVTMALGCNGRGIALCTSLGQQIAARLADSGHAFPYPVSPIVPIPLHGLQRFY
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
IAAGVAWYSLLDKLG
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMPSPDIVPDGEPLPPSLWAATAPAAPPTPALRESIATDVLVIGGGYTGLSTALHLAER
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHC
GTGVTVLEANDPGWGASGRNGGQVNPTLKHDPDELVRMLGAARAEPLIDAVSRSADLVFD
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LIGRHGIDCQPVRAGWLQLSYSDKAVPAMHARAHQWARRGVRVELLDRAAVARRVGTEAF
HHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHH
AGGWLDGRAGAIQPLAYARGLVHAAQRAGAAVHGNTAVTALERQGTAWIASTRTGAQVRA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCEEEEECCCCCEEEE
QHVVIATNGYTGALWPGLAQTVLSANSFIVATRPLKPADAQAILARGETASTSQRLLLYF
EEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCCCCCCCEEEEEE
RKDAAGRLLMGGRGFFAEPRGPQDFAHLERSLELLFPQLGRLDYAYRWAGRIAITRDFMP
ECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHC
HIHEPAPGVTMALGCNGRGIALCTSLGQQIAARLADSGHAFPYPVSPIVPIPLHGLQRFY
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
IAAGVAWYSLLDKLG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]