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The map label for this gene is dctA3

Identifier: 17547992

GI number: 17547992

Start: 3526243

End: 3527583

Strand: Direct

Name: dctA3

Synonym: RSc3275

Alternate gene names: 17547992

Gene position: 3526243-3527583 (Clockwise)

Preceding gene: 17547989

Following gene: 17547993

Centisome position: 94.88

GC content: 66.44

Gene sequence:

>1341_bases
ATGCAACGCTTCACGCGCCCGTTGTTCGGGCAGGTTTTGATCGCCCTCGCGCTGGGCATCGCGCTGGGCATCTGGGCGCC
CGATTTCGCGCAGCACCTCAAGCCACTGGGTGACGGCTTTCTGAAACTCATCAAGATGCTGATCGCGCCGATCGTGTTCT
CGGTGGTGGTGGTCGGCATCTGCGGCGCGGGCGAGCTCAAGAAGGTCGGGCGCGTGGGCGGCAAGGCCGTCATCTACTTC
GAGGTCGTGACCACCATTGCGCTGGCGTTGGGCATCGCGCTGGCCTATGCGTTCGGGCCCGGCCACGGCATGAACGTGGA
TCCGAAGACGCTCGACGCCTCGGCCATGTCGTCGTACATGGCGACGGCCAAGCAGGTGGAGTCGAGCGGCGTGGCGGAAT
TCCTGCTCAAGCTGATTCCCGACACCTTCGTCAGCGGCTTCATGAAGGGCGACATCCTGCAGGTGCTGCTGGTGTCGATC
CTGTTCGGCTGCGCGCTGTCGCTGCTGGGGGAGCGCACCAAGCCGCTGGTCGGCCTGATCGATCAGCTGTCCCACGTGCT
GTTCCGCATGATGGCCGTGGTGATCCGCCTGGCGCCGCTGGGCGTGCTGGGCGCGGTGGCCTTCACGGTGGGCAAGTACG
GCGCGGGGTCGCTCAAGCAGCTGGGTTTCCTGGTGCTGCTGTTCTACGCGGCGGTGGCGGTGTTCGTGGTGGTGGTGCTG
GGCGGCATCCTGCGGCTGGCCGGGTTCAGCATCTTCAAGCTGATCCGCTTCCTGCGCGCCGAGCTGCTGGTGGTGCTGGG
CACGGCCTCGTCCGATGCGGTGCTGCCCTCGGTCATGAACAAGCTGGAAAAGATGGGCATCAAGCGCTCGGTGGTGGGCC
TGGTGATCCCGACCGGCTATTCGTTCAACCTCGACGCGTTCTCGATCTACCTGACGCTGGCCGCCGTATTCATCGCGCAG
GCGACCAATACGCCGCTGGCGCTGTCGGACCTGCTGCTGATCCTGGGCGTGGCGCTGATCACCTCCAAGGGGGCGCACGG
CATCCCGGGCTCGGCCATCGTGATCCTGGCGGCGACGCTGTCGGTGATTCCCGCCATTCCGGCGATCGGCCTGGTGCTGG
TGCTGTCGGTGGACTGGTTCATCGGTATCGCCCGCGCACTGGGCAACCTGCTCGGCAACTGCGTCGCGACGGTGGTGATC
GCCGCCTGGGAAAAGGACATCGATCGCGCCCGGGCCAACGCGGTGCTCGACGGCAAGCTCGACATCATCGAGGAAGGCGA
GGCGGCGGCGCGCGGCCATGGCATCCAGGTGCCATCGGCGTCCACGCTGCCGCATGCCTGA

Upstream 100 bases:

>100_bases
ATCTGCTGCGGCGCATCGAGCGTGACAGAGCCTTTGGACCAATCGCGACAGACGGGCGGCCGGGGCTCGGACACCGTGCA
AATCCAAAGGAGGATTCACC

Downstream 100 bases:

>100_bases
GGCCGGGCGGACGGCGGTAAAATCGCGGTCATGACTGAAGAAACACAAGCCGCCGACCTTTCCTCCGAGGGCATTGCCGA
AGACATCGCCCAGGCGATCG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MQRFTRPLFGQVLIALALGIALGIWAPDFAQHLKPLGDGFLKLIKMLIAPIVFSVVVVGICGAGELKKVGRVGGKAVIYF
EVVTTIALALGIALAYAFGPGHGMNVDPKTLDASAMSSYMATAKQVESSGVAEFLLKLIPDTFVSGFMKGDILQVLLVSI
LFGCALSLLGERTKPLVGLIDQLSHVLFRMMAVVIRLAPLGVLGAVAFTVGKYGAGSLKQLGFLVLLFYAAVAVFVVVVL
GGILRLAGFSIFKLIRFLRAELLVVLGTASSDAVLPSVMNKLEKMGIKRSVVGLVIPTGYSFNLDAFSIYLTLAAVFIAQ
ATNTPLALSDLLLILGVALITSKGAHGIPGSAIVILAATLSVIPAIPAIGLVLVLSVDWFIGIARALGNLLGNCVATVVI
AAWEKDIDRARANAVLDGKLDIIEEGEAAARGHGIQVPSASTLPHA

Sequences:

>Translated_446_residues
MQRFTRPLFGQVLIALALGIALGIWAPDFAQHLKPLGDGFLKLIKMLIAPIVFSVVVVGICGAGELKKVGRVGGKAVIYF
EVVTTIALALGIALAYAFGPGHGMNVDPKTLDASAMSSYMATAKQVESSGVAEFLLKLIPDTFVSGFMKGDILQVLLVSI
LFGCALSLLGERTKPLVGLIDQLSHVLFRMMAVVIRLAPLGVLGAVAFTVGKYGAGSLKQLGFLVLLFYAAVAVFVVVVL
GGILRLAGFSIFKLIRFLRAELLVVLGTASSDAVLPSVMNKLEKMGIKRSVVGLVIPTGYSFNLDAFSIYLTLAAVFIAQ
ATNTPLALSDLLLILGVALITSKGAHGIPGSAIVILAATLSVIPAIPAIGLVLVLSVDWFIGIARALGNLLGNCVATVVI
AAWEKDIDRARANAVLDGKLDIIEEGEAAARGHGIQVPSASTLPHA
>Mature_446_residues
MQRFTRPLFGQVLIALALGIALGIWAPDFAQHLKPLGDGFLKLIKMLIAPIVFSVVVVGICGAGELKKVGRVGGKAVIYF
EVVTTIALALGIALAYAFGPGHGMNVDPKTLDASAMSSYMATAKQVESSGVAEFLLKLIPDTFVSGFMKGDILQVLLVSI
LFGCALSLLGERTKPLVGLIDQLSHVLFRMMAVVIRLAPLGVLGAVAFTVGKYGAGSLKQLGFLVLLFYAAVAVFVVVVL
GGILRLAGFSIFKLIRFLRAELLVVLGTASSDAVLPSVMNKLEKMGIKRSVVGLVIPTGYSFNLDAFSIYLTLAAVFIAQ
ATNTPLALSDLLLILGVALITSKGAHGIPGSAIVILAATLSVIPAIPAIGLVLVLSVDWFIGIARALGNLLGNCVATVVI
AAWEKDIDRARANAVLDGKLDIIEEGEAAARGHGIQVPSASTLPHA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.9443207126949, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4827012, Length=293, Percent_Identity=27.9863481228669, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI40254478, Length=423, Percent_Identity=24.5862884160757, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI194239697, Length=449, Percent_Identity=25.6124721603563, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI21314632, Length=399, Percent_Identity=27.8195488721804, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI66773030, Length=383, Percent_Identity=23.2375979112272, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI5032093, Length=399, Percent_Identity=26.5664160401003, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI223468566, Length=270, Percent_Identity=27.037037037037, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI223468564, Length=270, Percent_Identity=27.037037037037, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI262359914, Length=356, Percent_Identity=24.7191011235955, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI301601644, Length=164, Percent_Identity=32.3170731707317, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=50.9661835748792, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1790514, Length=435, Percent_Identity=41.1494252873563, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788024, Length=412, Percent_Identity=23.7864077669903, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789473, Length=235, Percent_Identity=25.9574468085106, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=473, Percent_Identity=24.9471458773784, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=441, Percent_Identity=24.0362811791383, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=498, Percent_Identity=22.6907630522088, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71996953, Length=449, Percent_Identity=25.1670378619154, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=453, Percent_Identity=22.2958057395143, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983106, Length=453, Percent_Identity=22.2958057395143, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=408, Percent_Identity=24.2647058823529, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24583025, Length=437, Percent_Identity=24.0274599542334, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137668, Length=437, Percent_Identity=24.0274599542334, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583023, Length=437, Percent_Identity=24.0274599542334, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281360483, Length=421, Percent_Identity=24.2280285035629, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17137666, Length=421, Percent_Identity=24.2280285035629, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281360481, Length=421, Percent_Identity=24.2280285035629, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA2_RALSO (Q8XUB6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_521394.1
- ProteinModelPortal:   Q8XUB6
- SMR:   Q8XUB6
- GeneID:   1222139
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc3275
- NMPDR:   fig|267608.1.peg.3273
- HOGENOM:   HBG739804
- OMA:   VLPQIMR
- ProtClustDB:   PRK13027
- BioCyc:   RSOL267608:RSC3275-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46572; Mature: 46572

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf6f1260)-; HASH(0x487351d8)-; HASH(0xf65f0d0)-; HASH(0xec5d340)-; HASH(0x48734d1c)-; HASH(0xf8301c0)-; HASH(0xf9c3cfc)-; HASH(0x48734c2c)-; HASH(0xe79cae0)-; HASH(0x487346e0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFTRPLFGQVLIALALGIALGIWAPDFAQHLKPLGDGFLKLIKMLIAPIVFSVVVVGI
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGAGELKKVGRVGGKAVIYFEVVTTIALALGIALAYAFGPGHGMNVDPKTLDASAMSSYM
CCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
ATAKQVESSGVAEFLLKLIPDTFVSGFMKGDILQVLLVSILFGCALSLLGERTKPLVGLI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DQLSHVLFRMMAVVIRLAPLGVLGAVAFTVGKYGAGSLKQLGFLVLLFYAAVAVFVVVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGILRLAGFSIFKLIRFLRAELLVVLGTASSDAVLPSVMNKLEKMGIKRSVVGLVIPTGY
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHHHHHEEECCCC
SFNLDAFSIYLTLAAVFIAQATNTPLALSDLLLILGVALITSKGAHGIPGSAIVILAATL
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SVIPAIPAIGLVLVLSVDWFIGIARALGNLLGNCVATVVIAAWEKDIDRARANAVLDGKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCC
DIIEEGEAAARGHGIQVPSASTLPHA
HHHCCCCHHHCCCCCCCCCCCCCCCH
>Mature Secondary Structure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HHHCCCCHHHCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852