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The map label for this gene is tuf [H]

Identifier: 17547760

GI number: 17547760

Start: 3271859

End: 3273049

Strand: Reverse

Name: tuf [H]

Synonym: RSc3041

Alternate gene names: 17547760

Gene position: 3273049-3271859 (Counterclockwise)

Preceding gene: 17547761

Following gene: 17547759

Centisome position: 88.07

GC content: 62.22

Gene sequence:

>1191_bases
ATGGCAAAGGAAAAGTTCGAGCGGACCAAGCCGCACGTGAACGTTGGGACGATCGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCAGCAATCGCGACGGTGCTGTCGAGCAAGTTCGGTGGCGAAGCGAAGAAGTACGACGAAATCGACGCAGCAC
CGGAAGAAAAGGCACGCGGCATCACGATCAACACCGCGCACATCGAGTACGAAACGGCCAACCGCCACTACGCACACGTC
GACTGCCCGGGCCACGCCGACTACGTCAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCCATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCCCGCCAGGTCGGCGTGCCGTACATCATCGTCT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAGCTGCTGGAACTGGTCGAAATGGAAGTGCGCGAGCTGCTCTCCAAG
TACGACTTCCCGGGCGACGACACCCCGATCATCAAGGGTTCGGCCAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAGTGGCCATCATGAACCTGGCCGACGCACTGGACTCGTACATCCCGACGCCGGAGCGCGCTGTTGACGGCACGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGCGGCACCGTGGTGACCGGCCGTATCGAGCGCGGCATCATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGCATCAAGGCCACGCAGAAGACCACCTGCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAAGGTCAGGCTGGCGACAACGTCGGTATCCTGCTGCGCGGCACCAAGCGCGAAGACGTCGAGCGCGGCCAAG
TGCTGTGCAAGCCGGGTTCGATCAAGCCGCACACGCACTTCACCGGCGAGGTCTACATCCTGTCGAAGGACGAAGGTGGT
CGTCACACCCCGTTCTTCAACAACTATCGCCCGCAGTTCTATTTCCGCACGACCGACGTGACCGGCTCGATCGAGCTGCC
GAAGGACAAGGAAATGGTCATGCCCGGCGACAACGTGTCGATCACCGTCAAGCTGATCGCCCCGATCGCCATGGAAGAAG
GCCTGCGCTTCGCCATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTCGTCGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
TAGAGCACTCCCTTGGTAAGGGAGAGGTCGGCAGTTCGATCCTGCCCATGGGCACCACGCATCCACCAACGCAAATCGCT
ACCGAGACAGGAGTCGCGAC

Downstream 100 bases:

>100_bases
TCAGGTAGTAATCTTCCGTGCGGCTGCCAACCGGCCGCACGTGAAGTGCCAGAGTGGGGTTGACCAAATGGCAACCCCAA
AGCTGTTTTAGGGGTATAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDSYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGIIK
VGEEIEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDSYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGIIK
VGEEIEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDSYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGIIKV
GEEIEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.3604060913706, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.8924485125858, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.8924485125858, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.7946428571429, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.5714285714286, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI46094014, Length=437, Percent_Identity=28.3752860411899, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018522, Length=443, Percent_Identity=28.4424379232506, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194018520, Length=443, Percent_Identity=28.4424379232506, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194097354, Length=443, Percent_Identity=28.4424379232506, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=29.2452830188679, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=32.0512820512821, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=32.0512820512821, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI157426893, Length=305, Percent_Identity=25.9016393442623, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80.7594936708861, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80.7594936708861, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=29.6187683284457, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=30.9090909090909, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789108, Length=146, Percent_Identity=34.2465753424658, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=25.4545454545455, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.3573200992556, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.4780361757106, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=461, Percent_Identity=31.4533622559653, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=461, Percent_Identity=31.4533622559653, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17506081, Length=295, Percent_Identity=30.5084745762712, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=29.6380090497738, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=26.4236902050114, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=26.4236902050114, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=28.169014084507, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=283, Percent_Identity=26.8551236749117, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.6565656565657, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.1801801801802, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.1801801801802, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=29.7935103244838, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=333, Percent_Identity=26.4264264264264, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.1698113207547, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.6875, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.7786259541985, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.7786259541985, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=52.8497409326425, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=29.4505494505495, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=29.4505494505495, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553807, Length=460, Percent_Identity=30, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553816, Length=460, Percent_Identity=30, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24651721, Length=460, Percent_Identity=30, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17864154, Length=460, Percent_Identity=30, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=31.0344827586207, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=27.2321428571429, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.5081967213115, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43168; Mature: 43037

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDSYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCEE
VGEEIEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDSYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCEE
VGEEIEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA