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The map label for this gene is mraW [H]

Identifier: 17547571

GI number: 17547571

Start: 3069680

End: 3070639

Strand: Reverse

Name: mraW [H]

Synonym: RSc2852

Alternate gene names: 17547571

Gene position: 3070639-3069680 (Counterclockwise)

Preceding gene: 17547572

Following gene: 17547570

Centisome position: 82.62

GC content: 65.1

Gene sequence:

>960_bases
ATGACGACCCAAGCCAGTACGGGACTGCGCCATCAAACGGTGCTGAGAGACGAAGCGATCGATGCGCTGTTGTGGCGCGA
CGACGGCATCTATATCGATGGAACCTTCGGGCGGGGCGGGCACAGCCGGCTCATCCTGGAGCGGCTGGGGCCCGGCGGAA
GGCTGATCGCTTTCGACAAGGACCCGGCAGCAATCACCGAAGCGGGCACCGTAGAGGATGCCCGCTTCGCTATTGAGCAT
GACAGCTTCGCGCACCTTGATGCTGCACTGGATGCGCGCGGTATTGGACGGGTGGCTGGCGTGCTGCTCGATCTGGGCAT
CAGCTCGCCGCAGATCGACGAAGGCGCGCGCGGCTTTTCGTTCCGGATGGACGGCCCGCTCGACATGCGGATGGACACCA
CGCGCGGCATCACTGCGGCCCAATGGCTGGCCGAGGCCGATGAGCGCGATATTGCGAGGGTGATACGGGACTATGGGGAA
GAACGGTTTGCTGTACAGATTGCAAAGGCGATTGTTGCTCGCCGGCGCGAATCCGGGACTCGAGGTCCTCTCGATCGCAC
ATCCGAGCTTGCCGCGCTCGTGGCGCAGGCCGTCAAGACACGCGAGAAGGGTCAAGACCCTGCGACCCGCACCTTTCAGG
CTCTACGGATTCACGTCAATCAAGAGCTTGCGGACCTCGAAACCGGTCTGAAGTCGGCATTCGAACGTCTGGAACAGGGG
GGACGACTCGTCGTGATCAGCTTCCATTCGCTGGAAGACCGCATCGTCAAGCGCTTCATGCAGGCACTGGCGCGGCCGGA
GCAATCCGCCGCGCCGGAGATGCGCCGGGCGCCGCTGCGTGCGCATGAGCTGCCGGCGCCGCAACTGCGCCTGCTGGGCC
GCGTGCGCCCGTCGGAGGCCGAGGTCTCGGCCAATCCGCGCTCGCGCTCGGCCATCATGCGCGTGGCCGAACGCTGCTGA

Upstream 100 bases:

>100_bases
CGAAATCTGGGACGCCGCCACGTACACCGCGAAGGAGCAGGCCGCCATGGCGCAAGGCATGCCCGACGCCCTCAAGAATT
TCTCTTTTTGATGACGCACG

Downstream 100 bases:

>100_bases
CCGACGGAGCCGCACGCCATGAACCGCCTCAACATGTTCCTGCTGACCGCGCTGGTGTTGTGCGCGCTGTCGCTGGTCAA
TGCGCAGCATCAGGCACGAC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTTQASTGLRHQTVLRDEAIDALLWRDDGIYIDGTFGRGGHSRLILERLGPGGRLIAFDKDPAAITEAGTVEDARFAIEH
DSFAHLDAALDARGIGRVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGE
ERFAVQIAKAIVARRRESGTRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKSAFERLEQG
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPEMRRAPLRAHELPAPQLRLLGRVRPSEAEVSANPRSRSAIMRVAERC

Sequences:

>Translated_319_residues
MTTQASTGLRHQTVLRDEAIDALLWRDDGIYIDGTFGRGGHSRLILERLGPGGRLIAFDKDPAAITEAGTVEDARFAIEH
DSFAHLDAALDARGIGRVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGE
ERFAVQIAKAIVARRRESGTRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKSAFERLEQG
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPEMRRAPLRAHELPAPQLRLLGRVRPSEAEVSANPRSRSAIMRVAERC
>Mature_318_residues
TTQASTGLRHQTVLRDEAIDALLWRDDGIYIDGTFGRGGHSRLILERLGPGGRLIAFDKDPAAITEAGTVEDARFAIEHD
SFAHLDAALDARGIGRVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEE
RFAVQIAKAIVARRRESGTRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKSAFERLEQGG
RLVVISFHSLEDRIVKRFMQALARPEQSAAPEMRRAPLRAHELPAPQLRLLGRVRPSEAEVSANPRSRSAIMRVAERC

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=344, Percent_Identity=39.5348837209302, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=38.8888888888889, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=56.0509554140127, Blast_Score=328, Evalue=3e-91,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=37.8787878787879, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=37.8787878787879, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35187; Mature: 35056

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQASTGLRHQTVLRDEAIDALLWRDDGIYIDGTFGRGGHSRLILERLGPGGRLIAFDK
CCCCCCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECC
DPAAITEAGTVEDARFAIEHDSFAHLDAALDARGIGRVAGVLLDLGISSPQIDEGARGFS
CCCHHCCCCCCCCHHEEECCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCE
FRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEERFAVQIAKAIVARRRESGT
EEECCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
RGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKSAFERLEQG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPEMRRAPLRAHELPAPQLRLLGRVRPSEA
CCEEEEEECCHHHHHHHHHHHHHCCCCHHCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC
EVSANPRSRSAIMRVAERC
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQASTGLRHQTVLRDEAIDALLWRDDGIYIDGTFGRGGHSRLILERLGPGGRLIAFDK
CCCCCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECC
DPAAITEAGTVEDARFAIEHDSFAHLDAALDARGIGRVAGVLLDLGISSPQIDEGARGFS
CCCHHCCCCCCCCHHEEECCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCE
FRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEERFAVQIAKAIVARRRESGT
EEECCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
RGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKSAFERLEQG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPEMRRAPLRAHELPAPQLRLLGRVRPSEA
CCEEEEEECCHHHHHHHHHHHHHCCCCHHCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC
EVSANPRSRSAIMRVAERC
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA