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The map label for this gene is ampD [H]

Identifier: 17547525

GI number: 17547525

Start: 3018877

End: 3019479

Strand: Reverse

Name: ampD [H]

Synonym: RSc2806

Alternate gene names: 17547525

Gene position: 3019479-3018877 (Counterclockwise)

Preceding gene: 17547526

Following gene: 17547524

Centisome position: 81.25

GC content: 70.81

Gene sequence:

>603_bases
ATGCCTGAGGTGGCCGGGCCGGTGCGCATCGGCGCGGACGGCTGGGCGGACGGGGTGTGCCGGCATCCTTCGCCCAATGT
CGATGCGCGCCCCGAGGGCCTGCCGATCGACCTGATCGTGCTGCACAACATCAGCCTGCCGCCCGCGCGTTTCGGCACCG
ACGACGTGCTGGATTTCTTCGCCAACACGCTCGACTGCGGCGCCCATCCCTACTTCGAGCAGTTGCGCGGCGTGCGGGTT
TCGGCGCACTTCTTCATCCGCCGCACCGGGGCGTGCGTGCAGTTCGCTCCCTGCGACGCGCGCGCCTGGCATGCCGGCGT
ATCGGATTTCTTCGGCCGCACGCGCTGCAACGACTTCTCCATCGGCATCGAGATCGAGGGGACGGACGACCAGCCCTTCA
CGCCCGCCCAGTACGCGGCGACGGCGGCCCTGGTGCGGGCGATCTGCGCGGCGTACCCGATCCGGGCAATCGCCGGGCAT
TCCGACATCGCGCCCGGGCGCAAGACGGACCCGGGGCCGTGCTTCGACTGGGCACACCTGCGCCGCCTCGCGAAACTGGA
GGCGGCACTGTTCCCGTACCAGGCAGGCGCGGCTCCGGCCTGA

Upstream 100 bases:

>100_bases
CTCGGCCTGAGCTTCCGGCAGCTGCGCTACCGGATGCAGCAGCTGGACATCCGCGATCCGCGCGACATCGAAGCCGCGGC
GGGCAACGGCGGGGTGGGTG

Downstream 100 bases:

>100_bases
TGAGCATGCGCGATCGCTTCGCGTTTGCGCATGTGATTTCTATCGAACTTGCTTGATCAATAGAAAGATTTATTCTCAAA
GCGAGTTCACCGCATCGCCG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPEVAGPVRIGADGWADGVCRHPSPNVDARPEGLPIDLIVLHNISLPPARFGTDDVLDFFANTLDCGAHPYFEQLRGVRV
SAHFFIRRTGACVQFAPCDARAWHAGVSDFFGRTRCNDFSIGIEIEGTDDQPFTPAQYAATAALVRAICAAYPIRAIAGH
SDIAPGRKTDPGPCFDWAHLRRLAKLEAALFPYQAGAAPA

Sequences:

>Translated_200_residues
MPEVAGPVRIGADGWADGVCRHPSPNVDARPEGLPIDLIVLHNISLPPARFGTDDVLDFFANTLDCGAHPYFEQLRGVRV
SAHFFIRRTGACVQFAPCDARAWHAGVSDFFGRTRCNDFSIGIEIEGTDDQPFTPAQYAATAALVRAICAAYPIRAIAGH
SDIAPGRKTDPGPCFDWAHLRRLAKLEAALFPYQAGAAPA
>Mature_199_residues
PEVAGPVRIGADGWADGVCRHPSPNVDARPEGLPIDLIVLHNISLPPARFGTDDVLDFFANTLDCGAHPYFEQLRGVRVS
AHFFIRRTGACVQFAPCDARAWHAGVSDFFGRTRCNDFSIGIEIEGTDDQPFTPAQYAATAALVRAICAAYPIRAIAGHS
DIAPGRKTDPGPCFDWAHLRRLAKLEAALFPYQAGAAPA

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=178, Percent_Identity=53.3707865168539, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787092, Length=143, Percent_Identity=37.7622377622378, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21555; Mature: 21423

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVAGPVRIGADGWADGVCRHPSPNVDARPEGLPIDLIVLHNISLPPARFGTDDVLDFF
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
ANTLDCGAHPYFEQLRGVRVSAHFFIRRTGACVQFAPCDARAWHAGVSDFFGRTRCNDFS
HHHHCCCCCHHHHHHCCCEEEEEEEEEECCCEEEECCCCCHHHHCCHHHHHCCCCCCCEE
IGIEIEGTDDQPFTPAQYAATAALVRAICAAYPIRAIAGHSDIAPGRKTDPGPCFDWAHL
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
RRLAKLEAALFPYQAGAAPA
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PEVAGPVRIGADGWADGVCRHPSPNVDARPEGLPIDLIVLHNISLPPARFGTDDVLDFF
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
ANTLDCGAHPYFEQLRGVRVSAHFFIRRTGACVQFAPCDARAWHAGVSDFFGRTRCNDFS
HHHHCCCCCHHHHHHCCCEEEEEEEEEECCCEEEECCCCCHHHHCCHHHHHCCCCCCCEE
IGIEIEGTDDQPFTPAQYAATAALVRAICAAYPIRAIAGHSDIAPGRKTDPGPCFDWAHL
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
RRLAKLEAALFPYQAGAAPA
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]