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The map label for this gene is mpl [H]

Identifier: 17547501

GI number: 17547501

Start: 2993040

End: 2994425

Strand: Reverse

Name: mpl [H]

Synonym: RSc2782

Alternate gene names: 17547501

Gene position: 2994425-2993040 (Counterclockwise)

Preceding gene: 17547512

Following gene: 17547500

Centisome position: 80.57

GC content: 66.31

Gene sequence:

>1386_bases
ATGCATATCCACATCCTTGGAATCTGCGGCACCTTCATGGGCGGGATCGCCCTCATCGCCCGCCAGGCCGGGCACCGCGT
CACCGGCTGCGACGCCAACGTCTACCCGCCGATGAGCACCCAGCTCGAAGCCCAGGGGATCGAGCTGATCGAGGGCTTCG
ATCCGGCGCAGTTGTCGGTCGGTGCCGATCTGTATGTGATTGGCAATGTGATCTCGCGGGGCAACCCGCTGATGGAAGCC
ATCCTCGACCGCAACCTGCCCTATGTCTCGGGGCCGCAGTGGCTGGGCGACAATGTGCTGCGGCAGAAGTGGGTGCTCGC
TGTAGCCGGCACACACGGCAAGACGACCACCACGTCGATGCTCGCCTGGATTCTGCAGGACGCCGGCTATGACCCGGGCT
TCCTGGTGGGCGGCGTGCCGCGCAACTTCGGGTTGTCGGCGCGGCTGACGGCGTCCGATTTCTTCGTCATCGAGGCCGAC
GAATACGACACGGCCTTCTTCGACAAGCGCAGCAAGTTCGTCCATTACCGTCCCCGTACGGCCATTCTGAACAATCTCGA
GTTCGATCACGCGGACATCTTTCCCGATCTTGCCGCTATCGAGACGCAATTCCACCATCTCGTGCGCACCGTTCCCGGTC
AGGGCCGCCTGCTGGTCAACGGCCGGGAAGACAGCCTGAAGCGTGTACTTGCACGCGGATGCTGGTCGGAAGCCGAGTTT
TTCGGTGCGGAAGACAGCGCAGGCTGGGCCATTGCCAATCTTGGCGATGACGACAGCTTCGACGTCATGCTCGACGGGCA
GGTTCAAGGCCGGGTGCGCTGGCCGCTGCAGGGCGACCACAACCGCATGAATGCGCTGGCCGCCATCGCCGCGGCGCGCC
ATGTCGGCGTGCCGCCCGCCCAGGCCATTGAAAGCCTGGCCCGTTTCGAGAATGTGAAACGGCGCATGGAGGTGCGCGGC
ACCGTGGACGGCATCACCGTCTACGACGATTTTGCCCACCACCCGACCGCCATCCGCACCACCATCGACGGCCTGCGCCG
TCGGGTGGGCGCCGCGCGCATCCTGGCCGTGCTGGAGCCACGTTCCAACACCATGAAGCTGGGCGTGATGAAGCAGCAGC
TTCCCGCCAGCCTGGAGGGCGCGGACCAGGTGTTTGCCTACGGCGCCGCCGGCGGCCGCGACGCGATCGGGTGGGATCTT
GCCGGCGCCCTGGCGCCGCTGGGCGAGCGGGCCCAGGCGTTCTCCGACCTCGTTGCGCTGGTCGAGGCCGTGACGGCGGC
CGCCCGACCCGGCGACCACGTGCTGGTGATGAGCAACGGCGGTTTCGGCGGCGTCCACCAGAAATTGCTGGATGCCCTGG
GTGAGCGGGTCGGTGCGGCAAACTGA

Upstream 100 bases:

>100_bases
CCGGACGCCGCCGCCCGCCTCCGGCGTGCCCCGGAAAACGGTCGCGGCATCGCCGGAGCCAGCAGGTACAATGCCGCGCA
ACGTAGTTGAGATGGCTCCC

Downstream 100 bases:

>100_bases
CGCGATATGCCGGCTTCCATCCTCTATCTGCACGGATTCCGCTCGTCGCCGCGCTCGTTCAAGGCGCAGCTGCTGGGCCA
GCGGATGGCCGCTGTCGGGC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso- diaminopimelate ligase transmembrane protein

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSVGADLYVIGNVISRGNPLMEA
ILDRNLPYVSGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYDPGFLVGGVPRNFGLSARLTASDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLARGCWSEAEF
FGAEDSAGWAIANLGDDDSFDVMLDGQVQGRVRWPLQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVDGITVYDDFAHHPTAIRTTIDGLRRRVGAARILAVLEPRSNTMKLGVMKQQLPASLEGADQVFAYGAAGGRDAIGWDL
AGALAPLGERAQAFSDLVALVEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALGERVGAAN

Sequences:

>Translated_461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSVGADLYVIGNVISRGNPLMEA
ILDRNLPYVSGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYDPGFLVGGVPRNFGLSARLTASDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLARGCWSEAEF
FGAEDSAGWAIANLGDDDSFDVMLDGQVQGRVRWPLQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVDGITVYDDFAHHPTAIRTTIDGLRRRVGAARILAVLEPRSNTMKLGVMKQQLPASLEGADQVFAYGAAGGRDAIGWDL
AGALAPLGERAQAFSDLVALVEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALGERVGAAN
>Mature_461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSVGADLYVIGNVISRGNPLMEA
ILDRNLPYVSGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYDPGFLVGGVPRNFGLSARLTASDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLARGCWSEAEF
FGAEDSAGWAIANLGDDDSFDVMLDGQVQGRVRWPLQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVDGITVYDDFAHHPTAIRTTIDGLRRRVGAARILAVLEPRSNTMKLGVMKQQLPASLEGADQVFAYGAAGGRDAIGWDL
AGALAPLGERAQAFSDLVALVEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALGERVGAAN

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=463, Percent_Identity=60.6911447084233, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1786279, Length=359, Percent_Identity=29.8050139275766, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49705; Mature: 49705

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSV
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEC
GADLYVIGNVISRGNPLMEAILDRNLPYVSGPQWLGDNVLRQKWVLAVAGTHGKTTTTSM
CCCEEEEHHHHHCCCHHHHHHHHCCCCCCCCCCHHCHHHHHHEEEEEEECCCCCCHHHHH
LAWILQDAGYDPGFLVGGVPRNFGLSARLTASDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEEECEEEEEECCCCCHHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLARGCWSEAEF
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
FGAEDSAGWAIANLGDDDSFDVMLDGQVQGRVRWPLQGDHNRMNALAAIAAARHVGVPPA
CCCCCCCCEEEEECCCCCCEEEEECCEECCEEECCCCCCCHHHHHHHHHHHHHHCCCCHH
QAIESLARFENVKRRMEVRGTVDGITVYDDFAHHPTAIRTTIDGLRRRVGAARILAVLEP
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
RSNTMKLGVMKQQLPASLEGADQVFAYGAAGGRDAIGWDLAGALAPLGERAQAFSDLVAL
CCCCEEEEEHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
VEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALGERVGAAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSV
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEC
GADLYVIGNVISRGNPLMEAILDRNLPYVSGPQWLGDNVLRQKWVLAVAGTHGKTTTTSM
CCCEEEEHHHHHCCCHHHHHHHHCCCCCCCCCCHHCHHHHHHEEEEEEECCCCCCHHHHH
LAWILQDAGYDPGFLVGGVPRNFGLSARLTASDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEEECEEEEEECCCCCHHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLARGCWSEAEF
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
FGAEDSAGWAIANLGDDDSFDVMLDGQVQGRVRWPLQGDHNRMNALAAIAAARHVGVPPA
CCCCCCCCEEEEECCCCCCEEEEECCEECCEEECCCCCCCHHHHHHHHHHHHHHCCCCHH
QAIESLARFENVKRRMEVRGTVDGITVYDDFAHHPTAIRTTIDGLRRRVGAARILAVLEP
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
RSNTMKLGVMKQQLPASLEGADQVFAYGAAGGRDAIGWDLAGALAPLGERAQAFSDLVAL
CCCCEEEEEHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
VEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALGERVGAAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]