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The map label for this gene is alkB [H]

Identifier: 17547287

GI number: 17547287

Start: 2776851

End: 2777507

Strand: Direct

Name: alkB [H]

Synonym: RSc2568

Alternate gene names: 17547287

Gene position: 2776851-2777507 (Clockwise)

Preceding gene: 17547286

Following gene: 17547288

Centisome position: 74.72

GC content: 72.3

Gene sequence:

>657_bases
ATGACGACCCGCGATCTCTTTGCCGACCATGCCCCCGCTGACGACCGCCGCATCGCGCTCGGTGAAGCGGCGGTCGTGCT
GCGCGGCTTTGCGCTGGCCGAGGCCACCGCGCTGCTCGCGGCCATCGACGACATCGCACGGCAGGCGCCGTTCCGCCACA
TGGTCACGCCGGGCGGCTTCGAGATGTCGGTCGCGCTCACCAACTGCGGCGCGCTCGGCTGGACCTCCGACCGCCGCGGC
TACCGCTATGCCGCGCGCGACCCGCAGACCGGTCAGCCCTGGCCGCCGCTGCCCGACTGCTTCCTGCGCCTGGCGCGCGA
TGCCGCCGCCGCAGCCGGCTTCCCAGGCTTCACGCCCGACGCCTGCCTCATCAACCGCTACGTGCCCGGTGCGCGCCTGT
CGCTGCACCAGGACAAGGACGAGCAGGATTACGGCGCGCCCATCGTGTCGGTCTCGCTCGGCATGCCGGCCATGTTCTTG
TGGGGTGGCCACCGGCGCACCGACAAGACGCTGCGCGTGCCGCTCTTCCACGGCGATGTGGTGGTCTGGGGCGGACCGGA
TCGCCTGCGCTACCACGGCGTCCTGCCGCTCAAGGAAGCCGCGCATCCGCTGCTGGGCGCGCAGCGCATCAACCTGACCT
TGCGCCGGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCCTACCGCGTCACCATGCGCCACGGCGTGAGCCGCCTGCGCTCGGGACAGCGGCATACTGTGGGCATCATCTTCCACGA
CGCCCTGTGACGCCGAGCGC

Downstream 100 bases:

>100_bases
CATGAGCATCGCCGCCGACCTGTCCGAACGCACCTACCGGCAAGCCACCGCGTTCCTGTCGTCGTTGGACGACGACTGGG
CGCGCCACATCGCCGCCATC

Product: alkylated DNA repair protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTTRDLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGFEMSVALTNCGALGWTSDRRG
YRYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPDACLINRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFL
WGGHRRTDKTLRVPLFHGDVVVWGGPDRLRYHGVLPLKEAAHPLLGAQRINLTLRRAG

Sequences:

>Translated_218_residues
MTTRDLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGFEMSVALTNCGALGWTSDRRG
YRYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPDACLINRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFL
WGGHRRTDKTLRVPLFHGDVVVWGGPDRLRYHGVLPLKEAAHPLLGAQRINLTLRRAG
>Mature_217_residues
TTRDLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGFEMSVALTNCGALGWTSDRRGY
RYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPDACLINRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFLW
GGHRRTDKTLRVPLFHGDVVVWGGPDRLRYHGVLPLKEAAHPLLGAQRINLTLRRAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=116, Percent_Identity=31.8965517241379, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1788541, Length=214, Percent_Identity=59.8130841121495, Blast_Score=255, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45555401, Length=99, Percent_Identity=38.3838383838384, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23731; Mature: 23600

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRDLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGF
CCCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
EMSVALTNCGALGWTSDRRGYRYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPD
EEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ACLINRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFLWGGHRRTDKTLRVPLFHGDV
HHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCE
VVWGGPDRLRYHGVLPLKEAAHPLLGAQRINLTLRRAG
EEECCCCCEEEECCCCHHHHHCHHCCCEEEEEEEEECC
>Mature Secondary Structure 
TTRDLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGF
CCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
EMSVALTNCGALGWTSDRRGYRYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPD
EEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ACLINRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFLWGGHRRTDKTLRVPLFHGDV
HHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCE
VVWGGPDRLRYHGVLPLKEAAHPLLGAQRINLTLRRAG
EEECCCCCEEEECCCCHHHHHCHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]