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The map label for this gene is ada [H]

Identifier: 17547285

GI number: 17547285

Start: 2775576

End: 2776091

Strand: Direct

Name: ada [H]

Synonym: RSc2566

Alternate gene names: 17547285

Gene position: 2775576-2776091 (Clockwise)

Preceding gene: 17547284

Following gene: 17547286

Centisome position: 74.68

GC content: 74.03

Gene sequence:

>516_bases
ATGAAACCCGCCGATTCCCCCACCCTGCGCTACGCCGTCGGCAAGGGCTCGCTCGGCCACGTTCTGGTTGCCACCCGGGG
ACACGGCCTCTGCGCGCTGCTGCTCGGCGACGATGCGAAGGCGCTGCGCGCGGCACTCGCCGACGCGTTTCCCGGCGCGA
CGCTGATCGAAGACCAGCCCGCGCTCGCCGCGCAACTGGCGCGCGCCATCGCCCTGGCCGAAGCCCCGTCGCTCGGCTTC
GGCGGCCCGCTCGACATGGGCGGCACGCCGTTCCAGCGCGCGGTCTGGCAGGCGCTGCACGACGTCCCGGCCGGCGCGAC
CGCCACCTACACCGAGATCGCCGAACGCATCGGCCGGCCCGACGCGGCGCACGCAGTGGCCGGCGCCTGCGCGGCCAACG
TGCTGGCGATCGCGATCCCGTGCCATCGCGCCGTCCGTGCGGATGGCGGACTGGCGGGGTATCGTTGGGGACTGGAACGC
AAGCGTGCGTTGCTGCAACGCGAGGCCCGCGCATGA

Upstream 100 bases:

>100_bases
GGACTGACCGGCGCCGAATTCAACCGCAAGCCCCGGAGTGCCGAGCCGGCACCGGGCACCTAACCTTCGCTTCATCAACA
ACTGCCCGATGGAGCGAAGC

Downstream 100 bases:

>100_bases
CCGGACTGCGAACCGCCACCCCTGGCGCGATCGACACAAGCGGCACAAGCGGCGCGCCGATCGACACCTTCGACTGGCGC
GCCGTGGAAGACGCGCTCAA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MKPADSPTLRYAVGKGSLGHVLVATRGHGLCALLLGDDAKALRAALADAFPGATLIEDQPALAAQLARAIALAEAPSLGF
GGPLDMGGTPFQRAVWQALHDVPAGATATYTEIAERIGRPDAAHAVAGACAANVLAIAIPCHRAVRADGGLAGYRWGLER
KRALLQREARA

Sequences:

>Translated_171_residues
MKPADSPTLRYAVGKGSLGHVLVATRGHGLCALLLGDDAKALRAALADAFPGATLIEDQPALAAQLARAIALAEAPSLGF
GGPLDMGGTPFQRAVWQALHDVPAGATATYTEIAERIGRPDAAHAVAGACAANVLAIAIPCHRAVRADGGLAGYRWGLER
KRALLQREARA
>Mature_171_residues
MKPADSPTLRYAVGKGSLGHVLVATRGHGLCALLLGDDAKALRAALADAFPGATLIEDQPALAAQLARAIALAEAPSLGF
GGPLDMGGTPFQRAVWQALHDVPAGATATYTEIAERIGRPDAAHAVAGACAANVLAIAIPCHRAVRADGGLAGYRWGLER
KRALLQREARA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=161, Percent_Identity=47.8260869565217, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1787596, Length=86, Percent_Identity=45.3488372093023, Blast_Score=87, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=123, Percent_Identity=39.8373983739837, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=123, Percent_Identity=40.650406504065, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=46.9879518072289, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=140, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17559; Mature: 17559

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPADSPTLRYAVGKGSLGHVLVATRGHGLCALLLGDDAKALRAALADAFPGATLIEDQP
CCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCH
ALAAQLARAIALAEAPSLGFGGPLDMGGTPFQRAVWQALHDVPAGATATYTEIAERIGRP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
DAAHAVAGACAANVLAIAIPCHRAVRADGGLAGYRWGLERKRALLQREARA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPADSPTLRYAVGKGSLGHVLVATRGHGLCALLLGDDAKALRAALADAFPGATLIEDQP
CCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCH
ALAAQLARAIALAEAPSLGFGGPLDMGGTPFQRAVWQALHDVPAGATATYTEIAERIGRP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
DAAHAVAGACAANVLAIAIPCHRAVRADGGLAGYRWGLERKRALLQREARA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]