Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 17547115

GI number: 17547115

Start: 2603122

End: 2603898

Strand: Reverse

Name: cobS

Synonym: RSc2396

Alternate gene names: 17547115

Gene position: 2603898-2603122 (Counterclockwise)

Preceding gene: 17547116

Following gene: 17547114

Centisome position: 70.06

GC content: 71.04

Gene sequence:

>777_bases
ATGATGGCGGCCCTGCGCGAGGCCTGCCGGTCGCTGTGGATCGCGATCGGCTATTTCACGCGCATTCCCGTGCCCGCATC
GGTCGGCTTCTCGCAGGACGGGCTGAACCGGGCGGCACGGTTTTTTCCGCTGGTGGGCTGGCTGGTCGGCGCCGCCGGCG
CGCTGGCGTATTGGCTGGCCTCGCGCACGGTGCCCGCGCCTGGCGTGGCGGTGGCGGCGTCGATGGCGGCCACGCTGCTG
TTGACCGGCGCTTTCCATGAAGACGGCCTCGCCGATTGCGCCGACGGCTTCGGCGGCGGCTATACCGCCGAAGACCGGCT
GCGCATCATGCGCGACTCGCGCATCGGCGCCTTTGGGGCTATTGCGGTGTGCATGGCCTTGCTGCTGAAGTGGCAACTGC
TGATGGCCATGGCGGCGCAGCACGCCGCGGCTGCGATGGCGGCCATGGTGGCCGCGCATGCCGCCAGCCGGGGCATGGCG
GTGAGCTATCTGCTCACGCACGACTACGCGCGCATGGAGGGCAAGGCCAAGCCCGTCGCGCAGCCCATGGGCCGGCGGGA
TGCGGCGTGGGCGGCCCTGTTCGGCGGACTGCCCCTGCTGGGGTTCGGCATGGCCTGCGCGGCCATCGCCGTGGCCGTGC
TGCTGGCGGCGCGGTGGGCGCTGGGACGCTACTTCACGCGGCGCCTCGGCGGCATCACCGGCGATTGCCTCGGGCTGGCC
CAGCAGGTCTTCGAACTGCTCGTACTGTGGGTGTTGCTGGCATGGACATCATCCTGA

Upstream 100 bases:

>100_bases
ATCCGCTGGTGGTCTCGGCGGTCGCCTTCCTGCGCGAGATGGCGACGTTCGCCAGCGCCGGCGTGAGCGAGCAGGCGCCG
CCGGCGCTGGATCCGGCGGC

Downstream 100 bases:

>100_bases
TCCGCCACGCGCGTCCGGCCATGGCCGCGGGCCTGTGCTACGGCCGCACCGATCTGCCGCTGGACGAGCCGATGGACCCC
GATGCCGCCAGCATCGCCGA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMAALREACRSLWIAIGYFTRIPVPASVGFSQDGLNRAARFFPLVGWLVGAAGALAYWLASRTVPAPGVAVAASMAATLL
LTGAFHEDGLADCADGFGGGYTAEDRLRIMRDSRIGAFGAIAVCMALLLKWQLLMAMAAQHAAAAMAAMVAAHAASRGMA
VSYLLTHDYARMEGKAKPVAQPMGRRDAAWAALFGGLPLLGFGMACAAIAVAVLLAARWALGRYFTRRLGGITGDCLGLA
QQVFELLVLWVLLAWTSS

Sequences:

>Translated_258_residues
MMAALREACRSLWIAIGYFTRIPVPASVGFSQDGLNRAARFFPLVGWLVGAAGALAYWLASRTVPAPGVAVAASMAATLL
LTGAFHEDGLADCADGFGGGYTAEDRLRIMRDSRIGAFGAIAVCMALLLKWQLLMAMAAQHAAAAMAAMVAAHAASRGMA
VSYLLTHDYARMEGKAKPVAQPMGRRDAAWAALFGGLPLLGFGMACAAIAVAVLLAARWALGRYFTRRLGGITGDCLGLA
QQVFELLVLWVLLAWTSS
>Mature_258_residues
MMAALREACRSLWIAIGYFTRIPVPASVGFSQDGLNRAARFFPLVGWLVGAAGALAYWLASRTVPAPGVAVAASMAATLL
LTGAFHEDGLADCADGFGGGYTAEDRLRIMRDSRIGAFGAIAVCMALLLKWQLLMAMAAQHAAAAMAAMVAAHAASRGMA
VSYLLTHDYARMEGKAKPVAQPMGRRDAAWAALFGGLPLLGFGMACAAIAVAVLLAARWALGRYFTRRLGGITGDCLGLA
QQVFELLVLWVLLAWTSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=35.8565737051793, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_RALSO (Q8XWS4)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520517.1
- GeneID:   1221242
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2396
- NMPDR:   fig|267608.1.peg.2396
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   PRK00235
- BioCyc:   RSOL267608:RSC2396-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27124; Mature: 27124

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe7c7734)-; HASH(0xe7f072c)-; HASH(0xe3d1734)-; HASH(0xe63b17c)-; HASH(0xe7840a8)-; HASH(0xe3a6138)-;

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAALREACRSLWIAIGYFTRIPVPASVGFSQDGLNRAARFFPLVGWLVGAAGALAYWLA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTVPAPGVAVAASMAATLLLTGAFHEDGLADCADGFGGGYTAEDRLRIMRDSRIGAFGA
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHH
IAVCMALLLKWQLLMAMAAQHAAAAMAAMVAAHAASRGMAVSYLLTHDYARMEGKAKPVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHH
QPMGRRDAAWAALFGGLPLLGFGMACAAIAVAVLLAARWALGRYFTRRLGGITGDCLGLA
HCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQVFELLVLWVLLAWTSS
HHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MMAALREACRSLWIAIGYFTRIPVPASVGFSQDGLNRAARFFPLVGWLVGAAGALAYWLA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTVPAPGVAVAASMAATLLLTGAFHEDGLADCADGFGGGYTAEDRLRIMRDSRIGAFGA
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHH
IAVCMALLLKWQLLMAMAAQHAAAAMAAMVAAHAASRGMAVSYLLTHDYARMEGKAKPVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHH
QPMGRRDAAWAALFGGLPLLGFGMACAAIAVAVLLAARWALGRYFTRRLGGITGDCLGLA
HCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQVFELLVLWVLLAWTSS
HHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11823852