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The map label for this gene is metG

Identifier: 17547100

GI number: 17547100

Start: 2585234

End: 2587300

Strand: Direct

Name: metG

Synonym: RSc2381

Alternate gene names: 17547100

Gene position: 2585234-2587300 (Clockwise)

Preceding gene: 17547099

Following gene: 17547104

Centisome position: 69.56

GC content: 64.63

Gene sequence:

>2067_bases
ATGTCCGAACGTCGCATCCTCGTCACATCCGCCCTGCCCTATGCCAACGGGCCGATCCACATCGGCCACATGGTCGAGTA
CATCCAGACCGACATCTGGGTGCGCTTCCAGCGCATGCGCGGCCACGAAACCTACTACGTGGGCGCCGACGACACGCATG
GCACACCCGTCATGCTGCGCGCCGAAAAGGAAGGCATCACCCCGCGCCAGCTGATCGAGCGCGTCTGGACGGAGCACAAG
CGCGACTTCGACAACTTCCTGGTCTCGTTCGACAACTACTACAGCACCGACTCCGACGAGAACCGCGAGCTGTGCGAGCG
CGTCTATCTCAAGCTCAAGGAGGCGGGCCTGATCGACGTGCGCGAGGTCGAGCAGTTCTATGACCCGGTCAAGGAGATGT
TCCTGCCGGACCGCTTCATCAAGGGCGAGTGCCCGAAGTGCGGCGCCAAGGACCAGTACGGCGATTCCTGCGAGGTGTGC
GGCGCGACCTACCAGCCGACCGACCTGAAGAACCCGTATTCGGTGGTGTCGGGCGCCACGCCGGTGCGCAAGTCTTCCGA
ACACTACTTCTTCAAGCTGTCCGACCCGCGCTGCGAGACCTTCCTGCGCGATTGGGTGGGCGACCTGGCCCAGCCCGAAG
CCACCAACAAGATGCGCGAATGGCTGGGTGACGAAGGCGAATCGACGCTGTCGGACTGGGACATTTCGCGCGATGCGCCG
TACTTCGGCTTCGAGATCCCGGGCGCGCCGGGCAAGTATTTCTACGTGTGGCTGGATGCGCCGGTCGGTTACTACGCCAG
CTTCAAGAACCTGTGCGGCAAGCTGGGACTCGACTTCGATGCGTGGGTGTCCACGCACTCGACCGCCGAGCAGTACCACT
TCATCGGCAAGGACATCCTGTACTTCCACACGCTGTTCTGGCCGGCGATGCTGCAGTTCTCGGGCCACCGCACGCCCACC
AACGTGTTCGCGCACGGCTTCCTGACGGTGGACGGCGCCAAGATGAGCAAGTCGCGCGGCACCTTCATCACCGCGCAGAG
CTATATCGACACGGGCCTGAACCCGGAATGGCTACGCTACTACTTCGCCGCCAAGCTCAACGCCACGATGGAAGACCTGG
ACCTGAACCTGGACGACTTCATCGCACGCGTGAACAGCGACCTGGTCGGCAAGTTCGTCAACATCGCCAGCCGCTCGGCG
GGTTTCCTGGTCAAGCGCTTCGAGGGCCGCGTGAACGATGCCGCGCTGGCCAACCCGCTGATGGTCCAACTGCGCGAAGC
CGCGCCGCAGATCGCCGACCTGTATGAGAAGCGCGAATACAGCAAGGCCCTGCGCACAGTGATGGAGCTGGCCGACGCCG
TGAACGCCTACGTCGACACCGAGAAGCCGTGGGACCTGGCCAAGGACGAAGCCCAGCGCGACAAGCTGCATGCCGCGTGC
TCGGTGGCGCTGGAAGCCTTCCGCCTGCTGGCGGTCTATCTCAAGCCGATCCTGCCGAGCACGGTCGCTCGCATCGAGGC
GTTCCTGAACATCGAGCCGCTGAACTGGCGCGCGATCGACGCGCAGCTGTCGTCGGCGCGACCGATCCAGCCGTATTCGC
ACCTGATGACGCGCGTGGACAAGAAGCAGGTCGATGCACTGCTGGAGGCCAACCGCCAGTCGCTGCAGGCCACGGTGGAT
GCGGTCCCGGCCGCCAACGGCACGCCTGCCGTCGAACCGATGGCCGAGACCATCGCCATCGACGATTTCGCCAAGATCGA
CCTGCGCGTGGCCAAGATCGTCGCCTGCCAGCGCGTGGAGGGCTCCAACAAGCTGCTGCAGCTGACGCTGGACGTGGGCG
AAGGCAAGACGCGCAACGTCTTCTCCGGCATCCAGTCGGCCTATGCGCCGGAAGACCTGGTCGGCAAGCTGACGGTGATG
GTGGCCAACCTGGCGCCGCGCAAGATGAAGTTCGGCATGTCGGAGGGCATGGTGCTGGCCGCCTCGGCCGCCGACGAAAA
GGCCCAGCCGGGCCTGTACATCCTTGAGCCGCACGCGGGCGCCGTGCCTGGCATGCGCGTCCGTTAA

Upstream 100 bases:

>100_bases
CCGTGCGCAGCCCGCCCCTGCCGGGCACCGCGCTAGAATATGCGTTTCCCGGCGCGCCGCCCGCCTCGTGGCCCCTGCCC
GCACTTTCCTGGTCCGTCCC

Downstream 100 bases:

>100_bases
GACACGCCCTGCCCGCCGCAGCGATCAGCCGCCGTTCAGGCGGTTGTCGCTGCGCGATGTCGTGGTGACGCGGCCGTCCG
GCCCGAAATGCACGTTGAAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSERRILVTSALPYANGPIHIGHMVEYIQTDIWVRFQRMRGHETYYVGADDTHGTPVMLRAEKEGITPRQLIERVWTEHK
RDFDNFLVSFDNYYSTDSDENRELCERVYLKLKEAGLIDVREVEQFYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVC
GATYQPTDLKNPYSVVSGATPVRKSSEHYFFKLSDPRCETFLRDWVGDLAQPEATNKMREWLGDEGESTLSDWDISRDAP
YFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCGKLGLDFDAWVSTHSTAEQYHFIGKDILYFHTLFWPAMLQFSGHRTPT
NVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDLDLNLDDFIARVNSDLVGKFVNIASRSA
GFLVKRFEGRVNDAALANPLMVQLREAAPQIADLYEKREYSKALRTVMELADAVNAYVDTEKPWDLAKDEAQRDKLHAAC
SVALEAFRLLAVYLKPILPSTVARIEAFLNIEPLNWRAIDAQLSSARPIQPYSHLMTRVDKKQVDALLEANRQSLQATVD
AVPAANGTPAVEPMAETIAIDDFAKIDLRVAKIVACQRVEGSNKLLQLTLDVGEGKTRNVFSGIQSAYAPEDLVGKLTVM
VANLAPRKMKFGMSEGMVLAASAADEKAQPGLYILEPHAGAVPGMRVR

Sequences:

>Translated_688_residues
MSERRILVTSALPYANGPIHIGHMVEYIQTDIWVRFQRMRGHETYYVGADDTHGTPVMLRAEKEGITPRQLIERVWTEHK
RDFDNFLVSFDNYYSTDSDENRELCERVYLKLKEAGLIDVREVEQFYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVC
GATYQPTDLKNPYSVVSGATPVRKSSEHYFFKLSDPRCETFLRDWVGDLAQPEATNKMREWLGDEGESTLSDWDISRDAP
YFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCGKLGLDFDAWVSTHSTAEQYHFIGKDILYFHTLFWPAMLQFSGHRTPT
NVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDLDLNLDDFIARVNSDLVGKFVNIASRSA
GFLVKRFEGRVNDAALANPLMVQLREAAPQIADLYEKREYSKALRTVMELADAVNAYVDTEKPWDLAKDEAQRDKLHAAC
SVALEAFRLLAVYLKPILPSTVARIEAFLNIEPLNWRAIDAQLSSARPIQPYSHLMTRVDKKQVDALLEANRQSLQATVD
AVPAANGTPAVEPMAETIAIDDFAKIDLRVAKIVACQRVEGSNKLLQLTLDVGEGKTRNVFSGIQSAYAPEDLVGKLTVM
VANLAPRKMKFGMSEGMVLAASAADEKAQPGLYILEPHAGAVPGMRVR
>Mature_687_residues
SERRILVTSALPYANGPIHIGHMVEYIQTDIWVRFQRMRGHETYYVGADDTHGTPVMLRAEKEGITPRQLIERVWTEHKR
DFDNFLVSFDNYYSTDSDENRELCERVYLKLKEAGLIDVREVEQFYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCG
ATYQPTDLKNPYSVVSGATPVRKSSEHYFFKLSDPRCETFLRDWVGDLAQPEATNKMREWLGDEGESTLSDWDISRDAPY
FGFEIPGAPGKYFYVWLDAPVGYYASFKNLCGKLGLDFDAWVSTHSTAEQYHFIGKDILYFHTLFWPAMLQFSGHRTPTN
VFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDLDLNLDDFIARVNSDLVGKFVNIASRSAG
FLVKRFEGRVNDAALANPLMVQLREAAPQIADLYEKREYSKALRTVMELADAVNAYVDTEKPWDLAKDEAQRDKLHAACS
VALEAFRLLAVYLKPILPSTVARIEAFLNIEPLNWRAIDAQLSSARPIQPYSHLMTRVDKKQVDALLEANRQSLQATVDA
VPAANGTPAVEPMAETIAIDDFAKIDLRVAKIVACQRVEGSNKLLQLTLDVGEGKTRNVFSGIQSAYAPEDLVGKLTVMV
ANLAPRKMKFGMSEGMVLAASAADEKAQPGLYILEPHAGAVPGMRVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=643, Percent_Identity=27.8382581648523, Blast_Score=238, Evalue=2e-62,
Organism=Homo sapiens, GI24308436, Length=537, Percent_Identity=26.6294227188082, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1788432, Length=690, Percent_Identity=55.3623188405797, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=112, Percent_Identity=36.6071428571429, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=656, Percent_Identity=28.2012195121951, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=526, Percent_Identity=25.0950570342205, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=421, Percent_Identity=24.7030878859857, Blast_Score=113, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=28.1914893617021, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321610, Length=547, Percent_Identity=24.6800731261426, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24655311, Length=602, Percent_Identity=28.9036544850498, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI21358483, Length=528, Percent_Identity=24.8106060606061, Blast_Score=129, Evalue=7e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_RALSO (Q8XWT9)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520502.1
- ProteinModelPortal:   Q8XWT9
- GeneID:   1221227
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2381
- NMPDR:   fig|267608.1.peg.2381
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- ProtClustDB:   PRK00133
- BioCyc:   RSOL267608:RSC2381-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77383; Mature: 77252

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERRILVTSALPYANGPIHIGHMVEYIQTDIWVRFQRMRGHETYYVGADDTHGTPVMLR
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEE
AEKEGITPRQLIERVWTEHKRDFDNFLVSFDNYYSTDSDENRELCERVYLKLKEAGLIDV
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
REVEQFYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCGATYQPTDLKNPYSVVSGAT
HHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCC
PVRKSSEHYFFKLSDPRCETFLRDWVGDLAQPEATNKMREWLGDEGESTLSDWDISRDAP
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
YFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCGKLGLDFDAWVSTHSTAEQYHFIGKDIL
CCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
YFHTLFWPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRY
HHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEHHHHCCCCCHHHHHH
YFAAKLNATMEDLDLNLDDFIARVNSDLVGKFVNIASRSAGFLVKRFEGRVNDAALANPL
HHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCHH
MVQLREAAPQIADLYEKREYSKALRTVMELADAVNAYVDTEKPWDLAKDEAQRDKLHAAC
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SVALEAFRLLAVYLKPILPSTVARIEAFLNIEPLNWRAIDAQLSSARPIQPYSHLMTRVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCCCCHHHHHHHHHH
KKQVDALLEANRQSLQATVDAVPAANGTPAVEPMAETIAIDDFAKIDLRVAKIVACQRVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC
GSNKLLQLTLDVGEGKTRNVFSGIQSAYAPEDLVGKLTVMVANLAPRKMKFGMSEGMVLA
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
ASAADEKAQPGLYILEPHAGAVPGMRVR
ECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SERRILVTSALPYANGPIHIGHMVEYIQTDIWVRFQRMRGHETYYVGADDTHGTPVMLR
CCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEE
AEKEGITPRQLIERVWTEHKRDFDNFLVSFDNYYSTDSDENRELCERVYLKLKEAGLIDV
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
REVEQFYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCGATYQPTDLKNPYSVVSGAT
HHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCC
PVRKSSEHYFFKLSDPRCETFLRDWVGDLAQPEATNKMREWLGDEGESTLSDWDISRDAP
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
YFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCGKLGLDFDAWVSTHSTAEQYHFIGKDIL
CCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
YFHTLFWPAMLQFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRY
HHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEHHHHCCCCCHHHHHH
YFAAKLNATMEDLDLNLDDFIARVNSDLVGKFVNIASRSAGFLVKRFEGRVNDAALANPL
HHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCHH
MVQLREAAPQIADLYEKREYSKALRTVMELADAVNAYVDTEKPWDLAKDEAQRDKLHAAC
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SVALEAFRLLAVYLKPILPSTVARIEAFLNIEPLNWRAIDAQLSSARPIQPYSHLMTRVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCCCCHHHHHHHHHH
KKQVDALLEANRQSLQATVDAVPAANGTPAVEPMAETIAIDDFAKIDLRVAKIVACQRVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC
GSNKLLQLTLDVGEGKTRNVFSGIQSAYAPEDLVGKLTVMVANLAPRKMKFGMSEGMVLA
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
ASAADEKAQPGLYILEPHAGAVPGMRVR
ECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852