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The map label for this gene is fdoI [H]

Identifier: 17547089

GI number: 17547089

Start: 2573281

End: 2574435

Strand: Reverse

Name: fdoI [H]

Synonym: RSc2370

Alternate gene names: 17547089

Gene position: 2574435-2573281 (Counterclockwise)

Preceding gene: 17547090

Following gene: 17547088

Centisome position: 69.27

GC content: 69.26

Gene sequence:

>1155_bases
ATGATTGCCTGCTGGAAACACCTGGCGATGGCGCTGCTGCTGGGCAGCGCGGCGGCGCTCGCATCGGCGCAGCAGCCGGC
CAGCGTGCCCGCCGTCGCGCCCGCCAATCCGGCCGTGCAGCCCGCGCAGCCGTTCGCCAACGTCGTCTCCGAGAGCGTCT
TCGACCTGCCCCGGCGCGACCTGGCCAAGGAGGCGCAGGACCAGACCGCGCGCCGCGTCACGCAGCCGGGCAACAACGCG
CCGGTCTGGCGCGAGGTCAATTCCGACCAGGCCGGCTACGCGAGCCTGCCCGGGCTGGAGAGCGGCGTGCTGATCCAGCG
TACCGGCCAGAAGTGGCGGCTGTTCCGCAACGGTGTCATCACCGTGTGGGGCGGCTGGCTGGTGGTGCTGGTGCCGCTGG
CGATCCTGGCGTTCTTCCTGCGGCGGGGCCAGATCCGCTTGAAGGCGGCGCCCACCGGCCGCCGGATCGAACGCTTCACG
CCCACCGAGCGTTACGTGCACTGGACCATGGCGATCAGCTTCGTCACGCTGGGCGTCTCGGGCATCGCGATGCTGTGGGG
CAAGCATTTCCTGCTGCCGCTGATGGGCCACCAGCTGTTCGGCTGGCTGACCTACGTGCTGAAGAACCTGCACAACCTGG
TCGGGCCGCTGTTCACCGTCAGCATCGTCGTCGCCTTCGTGATGTGGGCGCATGACAACCTGCCGCGCCGCGGCGATCTG
CAATGGCTGCTGCGCCTGGGCGGCATGTTCGGTGGCCATGGCGCCGAGGTGCCGTCGCATCGCTTCAACGCGGGCGAGAA
GCTGTGGTTCTGGGGCGGGCTGGTGGTGTTCGGGCTGATCCTGTCGGCCTCGGGCTGGGTGCTGGACCGCATCGTGCCGG
GCGTCGACTACTACCGCAGCACCATGCAGCTGGCCGAGATCGTCCACGCCATCGCCGCGGTGGGCATGATCTGCATGTCG
CTCGGCCACATCTATATCGGCACCATCGGCATGGAAGGCGCCTATCGCGCCATGCGCGAGGGCTATGTCGACGAGGCCTG
GGCCAAAGAGCACCACGTGCTCTGGTACGACGACATCCAGGCCGGCAGGATTCCCGCGCAGCGCTCCAAGCCGCCGGAGC
CGCCCGAGGCGCGCGAACAGCCGGCCAAGGGCTGA

Upstream 100 bases:

>100_bases
CGGGCGACCGCACGAGCTGGGAGAACCAGATCCGCGCGCGCGGCCAGTACCAGAACGAATACAACCGGACGCCCTGAGGC
GCGCGAGCGGAGGCCCGTCC

Downstream 100 bases:

>100_bases
CGCCATCGATCCGAGGAGCCCCGCCATGACATGGTCCACCAGGAAGATGCGCCGGTCGGGAACGTGGCTTGCTGCCGCGC
TGCTTGGCCTGGCGAGCGCC

Product: hypothetical protein

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIACWKHLAMALLLGSAAALASAQQPASVPAVAPANPAVQPAQPFANVVSESVFDLPRRDLAKEAQDQTARRVTQPGNNA
PVWREVNSDQAGYASLPGLESGVLIQRTGQKWRLFRNGVITVWGGWLVVLVPLAILAFFLRRGQIRLKAAPTGRRIERFT
PTERYVHWTMAISFVTLGVSGIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFVMWAHDNLPRRGDL
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWVLDRIVPGVDYYRSTMQLAEIVHAIAAVGMICMS
LGHIYIGTIGMEGAYRAMREGYVDEAWAKEHHVLWYDDIQAGRIPAQRSKPPEPPEAREQPAKG

Sequences:

>Translated_384_residues
MIACWKHLAMALLLGSAAALASAQQPASVPAVAPANPAVQPAQPFANVVSESVFDLPRRDLAKEAQDQTARRVTQPGNNA
PVWREVNSDQAGYASLPGLESGVLIQRTGQKWRLFRNGVITVWGGWLVVLVPLAILAFFLRRGQIRLKAAPTGRRIERFT
PTERYVHWTMAISFVTLGVSGIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFVMWAHDNLPRRGDL
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWVLDRIVPGVDYYRSTMQLAEIVHAIAAVGMICMS
LGHIYIGTIGMEGAYRAMREGYVDEAWAKEHHVLWYDDIQAGRIPAQRSKPPEPPEAREQPAKG
>Mature_384_residues
MIACWKHLAMALLLGSAAALASAQQPASVPAVAPANPAVQPAQPFANVVSESVFDLPRRDLAKEAQDQTARRVTQPGNNA
PVWREVNSDQAGYASLPGLESGVLIQRTGQKWRLFRNGVITVWGGWLVVLVPLAILAFFLRRGQIRLKAAPTGRRIERFT
PTERYVHWTMAISFVTLGVSGIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFVMWAHDNLPRRGDL
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWVLDRIVPGVDYYRSTMQLAEIVHAIAAVGMICMS
LGHIYIGTIGMEGAYRAMREGYVDEAWAKEHHVLWYDDIQAGRIPAQRSKPPEPPEAREQPAKG

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787750, Length=218, Percent_Identity=27.9816513761468, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 42432; Mature: 42432

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIACWKHLAMALLLGSAAALASAQQPASVPAVAPANPAVQPAQPFANVVSESVFDLPRRD
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
LAKEAQDQTARRVTQPGNNAPVWREVNSDQAGYASLPGLESGVLIQRTGQKWRLFRNGVI
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCEE
TVWGGWLVVLVPLAILAFFLRRGQIRLKAAPTGRRIERFTPTERYVHWTMAISFVTLGVS
EEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
GIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFVMWAHDNLPRRGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWVLDRIVPGVDYYRS
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
TMQLAEIVHAIAAVGMICMSLGHIYIGTIGMEGAYRAMREGYVDEAWAKEHHVLWYDDIQ
HHHHHHHHHHHHHHHHHHHHHCHHEEEEECCHHHHHHHHCCCCHHHHHCCCCEEEECCCC
AGRIPAQRSKPPEPPEAREQPAKG
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIACWKHLAMALLLGSAAALASAQQPASVPAVAPANPAVQPAQPFANVVSESVFDLPRRD
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
LAKEAQDQTARRVTQPGNNAPVWREVNSDQAGYASLPGLESGVLIQRTGQKWRLFRNGVI
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCEE
TVWGGWLVVLVPLAILAFFLRRGQIRLKAAPTGRRIERFTPTERYVHWTMAISFVTLGVS
EEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
GIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFVMWAHDNLPRRGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWVLDRIVPGVDYYRS
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
TMQLAEIVHAIAAVGMICMSLGHIYIGTIGMEGAYRAMREGYVDEAWAKEHHVLWYDDIQ
HHHHHHHHHHHHHHHHHHHHHCHHEEEEECCHHHHHHHHCCCCHHHHHCCCCEEEECCCC
AGRIPAQRSKPPEPPEAREQPAKG
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]