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The map label for this gene is stp [H]

Identifier: 17547042

GI number: 17547042

Start: 2522137

End: 2523702

Strand: Reverse

Name: stp [H]

Synonym: RSc2323

Alternate gene names: 17547042

Gene position: 2523702-2522137 (Counterclockwise)

Preceding gene: 17547043

Following gene: 17547040

Centisome position: 67.91

GC content: 63.03

Gene sequence:

>1566_bases
ATGCAGGCGCATGACATCGAGCAGCCAACCCGGCCGCAGACAGACCATCCCTTTGCCGCGCGCAACACCGTGGCGCTACT
GGCCGTATGCTTGGCCGCCCTGATGTTCGGGTTGGAGATTTCCAGCGTGCCGGTGATTCTCCCCACGCTGGAAGTGGCGC
TGCATGGCGATTTCAGCGGCATCCAGTGGATCATGAATGCCTACACCATCGCCTGCACTGCGGTATTGATGGCGACGGGA
ACGCTGGCGGACCGCTATGGCCGCAAACGCGTATTCCTCTACACGATCGCATTGTTTGGCGTCGCCTCGCTGCTGTGCGG
CCTGGCGTGGAATACGCCCATCCTGATCGCCAGCCGCTTCTTGCAGGGCGCGAGCGGTGGCGCGATGCTCATTTGCCTCG
TGGCAGTGCTCTCGCACCAGTTCCCCGAAGGCGCCGAACGCGGCAAGGCGTTCGGCATCTGGGGGATCGTCTTCGGCATC
GGGCTCGGCTTCGGACCGCTGATCGGTGGCCTGATCGTCGCCATGTCCGGTTGGAAGTGGGTCTTCTGGGTGCATGTTTT
CATCGCGGCCCTGACATTCGGCCTTGCCATCGTCGGCGTGCGCGAATCACGCGATCCGCATGCAAGGAGATTGGACCTGG
CTGGCATCGTCACCCTATCCCTCACGGTGCTGGGGCTTGCCTACTTCGTGACGCAGGGCGCCGACACAGGTTTCGGCAGC
GCTTCCGCCCTGAGGGTGATTGCAGCGACTGCGGTCAGTTTTGCGCTATTCCTTATCGTGGAAATGCGCGCCGCGCACCC
GATGTTCGACTTCTCGGTATTCCGGGTGCGCAATTTCTCGGGCGCGTTGCTTGGCTCAGTGGGCATGAACTTCAGCTTCT
GGCCGTTCATCATCTACCTGCCCATTTACTTCCAGAGTGCGCTGGGGCAAGGTGCAGCGGCAGCAGGCGTGTCGCTGCTG
GCCTATACCCTGCCTACGCTGGTGTTCCCACCCATCGGCGAGCGGCTGGCGTTGCGCTACCGGCCCGGCATCGTCATTCC
CGCCGGCTTGTTCACCATCGGGCTGGGGTTCTTCCTGATGCTCATTGGCAGCAGCATCGATCAGGCGAGTTGGCTGACCG
TACTGCCAGGCTGCCTGCTGGCTGGCGCGGGCTTGGGCATCACCAATACGCCTGTGACCAACACCACCACGGGCTCGGTG
TCGCCCGCACGCGCCGGTATGGCGTCCGGCATCGACATGAGCGCCCGCATGATCTCCCTGGCCATCAACATTGCTTTGAT
GGGATTTCTGCTGATGCAAAGGGTGCTGCAATATCTGCAAATCGCGCTGGTCAAACCGGCCGACGAAGCATCCTGGCGCG
CGCTGGCCAGCCGTATTGCGTCCGGGAATTTGCATGCCGCTGACGCTACCGCGTGGGCGCCCATGTCCCCAGAGACGCTG
GAGACAATCGGCCGCGCGGCGCTAGTGCACGGCTTCGGCGGGGTCATGCTCTACAGCGGCATCGCTGTCTGGGTGCTCGC
TGCTGCCAGCTTCCTGATCTTCTCCGGCAGAAAGGCGCAGCGATGA

Upstream 100 bases:

>100_bases
AGAACAACGCTGCACGAACGCTCCTCAGCATGCCGCCGAAGACGCGGCGCTGCCAGCGGGCAGCCAGACACCCTTCTCAA
CATCCCCGAGTGAAACAACC

Downstream 100 bases:

>100_bases
CCGGTCGCCACGCGGCGTCCTGCGTGGATCGCCGTGCTTGCCATCCTCGGCACCTTCGGGCGAGTCGTCTCTGACTGCGA
TGTGCCGGCAGTGCACACAA

Product: transporter transmembrane protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MQAHDIEQPTRPQTDHPFAARNTVALLAVCLAALMFGLEISSVPVILPTLEVALHGDFSGIQWIMNAYTIACTAVLMATG
TLADRYGRKRVFLYTIALFGVASLLCGLAWNTPILIASRFLQGASGGAMLICLVAVLSHQFPEGAERGKAFGIWGIVFGI
GLGFGPLIGGLIVAMSGWKWVFWVHVFIAALTFGLAIVGVRESRDPHARRLDLAGIVTLSLTVLGLAYFVTQGADTGFGS
ASALRVIAATAVSFALFLIVEMRAAHPMFDFSVFRVRNFSGALLGSVGMNFSFWPFIIYLPIYFQSALGQGAAAAGVSLL
AYTLPTLVFPPIGERLALRYRPGIVIPAGLFTIGLGFFLMLIGSSIDQASWLTVLPGCLLAGAGLGITNTPVTNTTTGSV
SPARAGMASGIDMSARMISLAINIALMGFLLMQRVLQYLQIALVKPADEASWRALASRIASGNLHAADATAWAPMSPETL
ETIGRAALVHGFGGVMLYSGIAVWVLAAASFLIFSGRKAQR

Sequences:

>Translated_521_residues
MQAHDIEQPTRPQTDHPFAARNTVALLAVCLAALMFGLEISSVPVILPTLEVALHGDFSGIQWIMNAYTIACTAVLMATG
TLADRYGRKRVFLYTIALFGVASLLCGLAWNTPILIASRFLQGASGGAMLICLVAVLSHQFPEGAERGKAFGIWGIVFGI
GLGFGPLIGGLIVAMSGWKWVFWVHVFIAALTFGLAIVGVRESRDPHARRLDLAGIVTLSLTVLGLAYFVTQGADTGFGS
ASALRVIAATAVSFALFLIVEMRAAHPMFDFSVFRVRNFSGALLGSVGMNFSFWPFIIYLPIYFQSALGQGAAAAGVSLL
AYTLPTLVFPPIGERLALRYRPGIVIPAGLFTIGLGFFLMLIGSSIDQASWLTVLPGCLLAGAGLGITNTPVTNTTTGSV
SPARAGMASGIDMSARMISLAINIALMGFLLMQRVLQYLQIALVKPADEASWRALASRIASGNLHAADATAWAPMSPETL
ETIGRAALVHGFGGVMLYSGIAVWVLAAASFLIFSGRKAQR
>Mature_521_residues
MQAHDIEQPTRPQTDHPFAARNTVALLAVCLAALMFGLEISSVPVILPTLEVALHGDFSGIQWIMNAYTIACTAVLMATG
TLADRYGRKRVFLYTIALFGVASLLCGLAWNTPILIASRFLQGASGGAMLICLVAVLSHQFPEGAERGKAFGIWGIVFGI
GLGFGPLIGGLIVAMSGWKWVFWVHVFIAALTFGLAIVGVRESRDPHARRLDLAGIVTLSLTVLGLAYFVTQGADTGFGS
ASALRVIAATAVSFALFLIVEMRAAHPMFDFSVFRVRNFSGALLGSVGMNFSFWPFIIYLPIYFQSALGQGAAAAGVSLL
AYTLPTLVFPPIGERLALRYRPGIVIPAGLFTIGLGFFLMLIGSSIDQASWLTVLPGCLLAGAGLGITNTPVTNTTTGSV
SPARAGMASGIDMSARMISLAINIALMGFLLMQRVLQYLQIALVKPADEASWRALASRIASGNLHAADATAWAPMSPETL
ETIGRAALVHGFGGVMLYSGIAVWVLAAASFLIFSGRKAQR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=27.3809523809524, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1788710, Length=316, Percent_Identity=28.7974683544304, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789042, Length=336, Percent_Identity=27.0833333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790195, Length=411, Percent_Identity=23.1143552311436, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788392, Length=423, Percent_Identity=25.7683215130024, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790146, Length=158, Percent_Identity=27.2151898734177, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=294, Percent_Identity=25.5102040816327, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55146; Mature: 55146

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAHDIEQPTRPQTDHPFAARNTVALLAVCLAALMFGLEISSVPVILPTLEVALHGDFSG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHH
IQWIMNAYTIACTAVLMATGTLADRYGRKRVFLYTIALFGVASLLCGLAWNTPILIASRF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LQGASGGAMLICLVAVLSHQFPEGAERGKAFGIWGIVFGIGLGFGPLIGGLIVAMSGWKW
HCCCCCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHCCCHH
VFWVHVFIAALTFGLAIVGVRESRDPHARRLDLAGIVTLSLTVLGLAYFVTQGADTGFGS
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ASALRVIAATAVSFALFLIVEMRAAHPMFDFSVFRVRNFSGALLGSVGMNFSFWPFIIYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHECCCCCHHHHHCCCCCCHHHHHHHH
PIYFQSALGQGAAAAGVSLLAYTLPTLVFPPIGERLALRYRPGIVIPAGLFTIGLGFFLM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECHHHHHHHHHHHHH
LIGSSIDQASWLTVLPGCLLAGAGLGITNTPVTNTTTGSVSPARAGMASGIDMSARMISL
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHH
AINIALMGFLLMQRVLQYLQIALVKPADEASWRALASRIASGNLHAADATAWAPMSPETL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ETIGRAALVHGFGGVMLYSGIAVWVLAAASFLIFSGRKAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQAHDIEQPTRPQTDHPFAARNTVALLAVCLAALMFGLEISSVPVILPTLEVALHGDFSG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHH
IQWIMNAYTIACTAVLMATGTLADRYGRKRVFLYTIALFGVASLLCGLAWNTPILIASRF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LQGASGGAMLICLVAVLSHQFPEGAERGKAFGIWGIVFGIGLGFGPLIGGLIVAMSGWKW
HCCCCCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHCCCHH
VFWVHVFIAALTFGLAIVGVRESRDPHARRLDLAGIVTLSLTVLGLAYFVTQGADTGFGS
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ASALRVIAATAVSFALFLIVEMRAAHPMFDFSVFRVRNFSGALLGSVGMNFSFWPFIIYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHECCCCCHHHHHCCCCCCHHHHHHHH
PIYFQSALGQGAAAAGVSLLAYTLPTLVFPPIGERLALRYRPGIVIPAGLFTIGLGFFLM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECHHHHHHHHHHHHH
LIGSSIDQASWLTVLPGCLLAGAGLGITNTPVTNTTTGSVSPARAGMASGIDMSARMISL
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHH
AINIALMGFLLMQRVLQYLQIALVKPADEASWRALASRIASGNLHAADATAWAPMSPETL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ETIGRAALVHGFGGVMLYSGIAVWVLAAASFLIFSGRKAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]