Click here to switch to the map view.

The map label for this gene is gluQ

Identifier: 17546929

GI number: 17546929

Start: 2394058

End: 2395005

Strand: Direct

Name: gluQ

Synonym: RSc2210

Alternate gene names: 17546929

Gene position: 2394058-2395005 (Clockwise)

Preceding gene: 17546928

Following gene: 17546931

Centisome position: 64.42

GC content: 70.99

Gene sequence:

>948_bases
TTGTCCCAAGCCAATCTCGAATTCCCCGAATCCGTTGCAGCGGCCCCGCGCGGGCCGTATCGCGGCCGCTTCGCGCCGTC
GCCCACGGGGCCGCTGCACATCGGTTCGCTGGTCACCGCGCTGGCGAGCTGGCTCGATGCGCGCGTGCATGGCGGCACGT
GGCTCGTACGCATTGAAGACATCGATTTCCAGCGCAATGTGCCCGGCGCCGACCGCGACATCCTTGCCTCGCTCGAAACG
CTGGGGCTCGTGCCGGACGAAGCGCCGCAGTGGCAAAGCGCGCACCTGCCCCGCTTCGAAGCGGCGCTGGCGCAGCTGCA
GGCGATCGACCGGCTCTATCCGTGCGGCTGCACCCGCCGGGAAATCGCCGACTCCATCACTACGGTCGATGCGCAGGGCC
GCCTGCGCCACCAGACCCTGATCTACCCCGGCACCTGCCGCAACGGCCTGATGGGCCGCCCGCCGCGCGCCTGGCGCGTG
CGCCTGCCGGACGCCGATGCCGCCACGGTCTGCTTCGACGACCGCTGGCAAGGCACGCAATGCCAGAACCTGGCCGAAGC
GGTGGGCGATTTCGTGCTCAAGCGCGCCGACGGCATGTGGGCGTACCAGATCGCGGTGGTGGTGGACGATGCCGCGCAGG
GCATCACGGATGTGGTGCGCGGCGCCGACCTGCTCGATTCGACGCCGCGCCAGATCTACCTGCAGCGGCTGCTGGGGCTG
CCCGCGGTGCGCTACCTGCACGTGCCGGTGGTGGTCAACGCCGCCGGCGAGAAGCTCAGCAAGCAGACCGGCGCACGGGC
CATCAACCGCAGCCAGCCGCTGGCCGCGCTGACCGAAGCGGCGCGGCACCTGGGGCTGGAGATCCGCGCAGCGGACATGG
AAGGGTTCTACCGGGCGGCCGTCCCGGCATGGGCCCATCGCTTGGCCCAGTTGACGCCGGCGGCCTAG

Upstream 100 bases:

>100_bases
CTTGAAGACCCGCGCCTGGCGCAGGCCCTGCTGATGCAGTAGACCCTCCGATCGCGCACAATAGCGCCATCGGCCCTTGC
CCGACGCCCGGAGTCCCGCG

Downstream 100 bases:

>100_bases
CAGCGGCCACCGCGCTCAGTGCAGCAAGACCGCTCAGCGCTTGCGCGGCACGCCGCCCAGCAGGGCGGCAATCGGGCGCT
TGGCGCTGCGCGGGTGACCG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSQANLEFPESVAAAPRGPYRGRFAPSPTGPLHIGSLVTALASWLDARVHGGTWLVRIEDIDFQRNVPGADRDILASLET
LGLVPDEAPQWQSAHLPRFEAALAQLQAIDRLYPCGCTRREIADSITTVDAQGRLRHQTLIYPGTCRNGLMGRPPRAWRV
RLPDADAATVCFDDRWQGTQCQNLAEAVGDFVLKRADGMWAYQIAVVVDDAAQGITDVVRGADLLDSTPRQIYLQRLLGL
PAVRYLHVPVVVNAAGEKLSKQTGARAINRSQPLAALTEAARHLGLEIRAADMEGFYRAAVPAWAHRLAQLTPAA

Sequences:

>Translated_315_residues
MSQANLEFPESVAAAPRGPYRGRFAPSPTGPLHIGSLVTALASWLDARVHGGTWLVRIEDIDFQRNVPGADRDILASLET
LGLVPDEAPQWQSAHLPRFEAALAQLQAIDRLYPCGCTRREIADSITTVDAQGRLRHQTLIYPGTCRNGLMGRPPRAWRV
RLPDADAATVCFDDRWQGTQCQNLAEAVGDFVLKRADGMWAYQIAVVVDDAAQGITDVVRGADLLDSTPRQIYLQRLLGL
PAVRYLHVPVVVNAAGEKLSKQTGARAINRSQPLAALTEAARHLGLEIRAADMEGFYRAAVPAWAHRLAQLTPAA
>Mature_314_residues
SQANLEFPESVAAAPRGPYRGRFAPSPTGPLHIGSLVTALASWLDARVHGGTWLVRIEDIDFQRNVPGADRDILASLETL
GLVPDEAPQWQSAHLPRFEAALAQLQAIDRLYPCGCTRREIADSITTVDAQGRLRHQTLIYPGTCRNGLMGRPPRAWRVR
LPDADAATVCFDDRWQGTQCQNLAEAVGDFVLKRADGMWAYQIAVVVDDAAQGITDVVRGADLLDSTPRQIYLQRLLGLP
AVRYLHVPVVVNAAGEKLSKQTGARAINRSQPLAALTEAARHLGLEIRAADMEGFYRAAVPAWAHRLAQLTPAA

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=260, Percent_Identity=35.3846153846154, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI226510925, Length=289, Percent_Identity=41.1764705882353, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1788743, Length=261, Percent_Identity=35.2490421455939, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71993394, Length=296, Percent_Identity=32.4324324324324, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324540, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24665321, Length=264, Percent_Identity=35.2272727272727, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_RALSO (Q8XXA6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520331.1
- ProteinModelPortal:   Q8XXA6
- SMR:   Q8XXA6
- GeneID:   1221054
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2210
- NMPDR:   fig|267608.1.peg.2210
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- ProtClustDB:   PRK05710
- BioCyc:   RSOL267608:RSC2210-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 34361; Mature: 34229

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 59-59 BINDING 202-202 BINDING 220-220 BINDING 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQANLEFPESVAAAPRGPYRGRFAPSPTGPLHIGSLVTALASWLDARVHGGTWLVRIED
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
IDFQRNVPGADRDILASLETLGLVPDEAPQWQSAHLPRFEAALAQLQAIDRLYPCGCTRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
EIADSITTVDAQGRLRHQTLIYPGTCRNGLMGRPPRAWRVRLPDADAATVCFDDRWQGTQ
HHHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCH
CQNLAEAVGDFVLKRADGMWAYQIAVVVDDAAQGITDVVRGADLLDSTPRQIYLQRLLGL
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHCHHHHCCCHHHHHHHHHHCC
PAVRYLHVPVVVNAAGEKLSKQTGARAINRSQPLAALTEAARHLGLEIRAADMEGFYRAA
CCEEEEEECEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHH
VPAWAHRLAQLTPAA
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQANLEFPESVAAAPRGPYRGRFAPSPTGPLHIGSLVTALASWLDARVHGGTWLVRIED
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
IDFQRNVPGADRDILASLETLGLVPDEAPQWQSAHLPRFEAALAQLQAIDRLYPCGCTRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
EIADSITTVDAQGRLRHQTLIYPGTCRNGLMGRPPRAWRVRLPDADAATVCFDDRWQGTQ
HHHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCH
CQNLAEAVGDFVLKRADGMWAYQIAVVVDDAAQGITDVVRGADLLDSTPRQIYLQRLLGL
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHCHHHHCCCHHHHHHHHHHCC
PAVRYLHVPVVVNAAGEKLSKQTGARAINRSQPLAALTEAARHLGLEIRAADMEGFYRAA
CCEEEEEECEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHH
VPAWAHRLAQLTPAA
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852