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The map label for this gene is phaZ1 [H]

Identifier: 17546868

GI number: 17546868

Start: 2332231

End: 2333262

Strand: Direct

Name: phaZ1 [H]

Synonym: RSc2149

Alternate gene names: 17546868

Gene position: 2332231-2333262 (Clockwise)

Preceding gene: 17546858

Following gene: 17546876

Centisome position: 62.75

GC content: 72.29

Gene sequence:

>1032_bases
ATGAAGTCCGTCTCCCGGCTGGTCAATGCGTTGATCCCGACCAGCCTGGGCCGCCCGGCCTGGCCGCCGAAACCGGTCAA
GCCGGCCAGGCCCGCCACCCGGCGCCCCCGCCCGGCCGTCACACCGACCCCGACCGCCGGCACCTTCGTGCGCGAACGCT
TCATCTACGAGCCGGAATCTCCCGCCGTGGTGGCCCGTCGGCCCGAGTACTGGCTGTACACGCCGCCCTCGCGCGGCAGC
ACGCCACCCGCGCTGATGGTGATGCTGCATGGCTGCAAGCAGTCGGCCGAGGCGCTTGCCCACGGTACGCGCATGAACGC
GCTGGCCGACCGCGAAGGCTTCATCGTGCTGTACCCCGAGCAGCCGCGCCGCGCGCATCCGCAATGCTGCTGGCACTGGT
ATGACCCCGAACACGCCGGTGCGCACGAAGCGGATGACCTTGCCCGGCTCGTGGCGCAAACCCTGACCGAGACCGGCGCC
GACCCCTCGCGCGTCTATCTCGCGGGGCTGTCCGCGGGTGCCGGGCTGGCCGGGCTGATGGCGCTGCGGCACCCTCACCT
GTTCGCCGCCCTGGCCCTGCACTCCGGACCGGTGCTCGGCGTCGCCCGCACGGCCGCCTCGGCGCTGAACCTCATGCGGC
GGGGCGCGCGCATCGACCCCGTCGAAGCCCTGGCCACGATGGTCGACACCGGCACCTATCCCGGTATCCCGACGCTGCTG
CTGCACGGTCTGCGCGACGAGGCCGTCAGTCCTGTCAACCAGGCCCAGCTGGCAACCCAGTTCCGCGCCCTCAACCATCT
GTGCGACGCCGCCGACGTGCGGCGCGAAACCACGCGCGGCGACGGCTACGTCCTGCGCAGCGATCTGCGCGACGACGCGT
GCCTGCTGGCCACCTGCCAGTTCACCAGCACCGGGCACGCATGGAGCGGCGGCGATCCGCGCTACGCCTTCCACGCGGCA
GGCCCCGATGCGACCTGGCTGTGGTGGCAATTCGCGCGACAGCACGCGCGCACCACCGCACCCGCCACCTGA

Upstream 100 bases:

>100_bases
GGCACAATGGTTCGCAATCGACTTTCGCGACCGCCCCGAACGGGCGTGCGCCCCTGCGCGTCCCGCCCAGCTACACGCCG
CCGCCATGCACCGAACGTGG

Downstream 100 bases:

>100_bases
GCGCGGCGAAGCGCGCGCTACAGATCGAAGGACGACTGCCCGCCGCCACGGCTGAGCAGCACATCGACCAGACCGTAGCC
GCGCGAAAAACCCGCATCCT

Product: hypothetical protein

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKSVSRLVNALIPTSLGRPAWPPKPVKPARPATRRPRPAVTPTPTAGTFVRERFIYEPESPAVVARRPEYWLYTPPSRGS
TPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAHEADDLARLVAQTLTETGA
DPSRVYLAGLSAGAGLAGLMALRHPHLFAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLL
LHGLRDEAVSPVNQAQLATQFRALNHLCDAADVRRETTRGDGYVLRSDLRDDACLLATCQFTSTGHAWSGGDPRYAFHAA
GPDATWLWWQFARQHARTTAPAT

Sequences:

>Translated_343_residues
MKSVSRLVNALIPTSLGRPAWPPKPVKPARPATRRPRPAVTPTPTAGTFVRERFIYEPESPAVVARRPEYWLYTPPSRGS
TPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAHEADDLARLVAQTLTETGA
DPSRVYLAGLSAGAGLAGLMALRHPHLFAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLL
LHGLRDEAVSPVNQAQLATQFRALNHLCDAADVRRETTRGDGYVLRSDLRDDACLLATCQFTSTGHAWSGGDPRYAFHAA
GPDATWLWWQFARQHARTTAPAT
>Mature_343_residues
MKSVSRLVNALIPTSLGRPAWPPKPVKPARPATRRPRPAVTPTPTAGTFVRERFIYEPESPAVVARRPEYWLYTPPSRGS
TPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAHEADDLARLVAQTLTETGA
DPSRVYLAGLSAGAGLAGLMALRHPHLFAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLL
LHGLRDEAVSPVNQAQLATQFRALNHLCDAADVRRETTRGDGYVLRSDLRDDACLLATCQFTSTGHAWSGGDPRYAFHAA
GPDATWLWWQFARQHARTTAPAT

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37297; Mature: 37297

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVSRLVNALIPTSLGRPAWPPKPVKPARPATRRPRPAVTPTPTAGTFVRERFIYEPES
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PAVVARRPEYWLYTPPSRGSTPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPE
CEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCC
QPRRAHPQCCWHWYDPEHAGAHEADDLARLVAQTLTETGADPSRVYLAGLSAGAGLAGLM
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
ALRHPHLFAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLL
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHH
LHGLRDEAVSPVNQAQLATQFRALNHLCDAADVRRETTRGDGYVLRSDLRDDACLLATCQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEE
FTSTGHAWSGGDPRYAFHAAGPDATWLWWQFARQHARTTAPAT
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSVSRLVNALIPTSLGRPAWPPKPVKPARPATRRPRPAVTPTPTAGTFVRERFIYEPES
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PAVVARRPEYWLYTPPSRGSTPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPE
CEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCC
QPRRAHPQCCWHWYDPEHAGAHEADDLARLVAQTLTETGADPSRVYLAGLSAGAGLAGLM
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
ALRHPHLFAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLL
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHH
LHGLRDEAVSPVNQAQLATQFRALNHLCDAADVRRETTRGDGYVLRSDLRDDACLLATCQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEE
FTSTGHAWSGGDPRYAFHAAGPDATWLWWQFARQHARTTAPAT
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]