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The map label for this gene is ilvI [H]

Identifier: 17546796

GI number: 17546796

Start: 2249827

End: 2251584

Strand: Reverse

Name: ilvI [H]

Synonym: RSc2077

Alternate gene names: 17546796

Gene position: 2251584-2249827 (Counterclockwise)

Preceding gene: 17546808

Following gene: 17546795

Centisome position: 60.58

GC content: 63.48

Gene sequence:

>1758_bases
ATGAATATGCCAAGCGCGGAATTTTCCCACGCCAATAGCGGTTCACCTGCCGAAATGATGGGGTCTGACATCCTCGTCAA
TGCGCTCGCGGCAGAAGGCGTCGAGTTCGTCTGGGGTTACCCGGGCGGCGCGGTGCTCTATATCTACGACGCGTTCTACA
AGCAGAACAAGATCGAACACATCCTGGTGCGCCACGAGCAGGCGGCGGTCCATGCGGCCGACGGCTATGCGCGCGCCACG
GGCAAGGTCGGGGTCGCCCTGGTGACGTCCGGCCCGGGCGTGACCAATGCCGTGACCGGCATCGCCACCGCCTACCTGGA
TTCGATCCCGATGGTGATCATCACCGGCAACGTGCCGACGCACGCCATCGGCCAGGACGCCTTCCAGGAATGCGACACCG
TCGGCATCACGCGCCCGATCGTCAAGCACAACTTCCTGGTCAAGGACGTGCGCAACCTCGCTTCGACCATCAAGAAGGCG
TTCTACATCGCCTCGACCGGCCGTCCGGGTCCGGTGGTGGTGGACATCCCGAAGGATGTCTCGCGCGAGCTGTGCAAGTA
CGAGTATCCGAAGACCATCGAGATGCGCTCGTACAACCCGGTCAACAAGGGCCACTCCGGCCAGATCCGCAAGGCCGTGA
GCCTGCTGCTGCAGGCCGAGCGTCCGTACATCTACACCGGCGGCGGGGTGGTGCTGGCCAATGCCAGCGAAGAGCTGCGC
CAGCTGGCGGCCCTCACCGGCTACCCGGTGACGAACACGCTGATGGGTCTGGGCGCCTTCCCCGGCGCCAGCAAGCAGTT
CCTCGGCATGCTGGGCATGCATGGCACGTACGAAGCCAACATGGCCATGCAGCACTGCGACGTGCTGATCGCCGTCGGCG
CGCGCTTCGATGACCGCGTGATCGGCAGCCCGGCGCACTTCGCCTCGCAGCCGCGCAAGATCATCCACATCGACATCGAT
CCGTCGTCCATTTCCAAGCGGGTCAAGGTGGACATCCCCATCGTCGGCAACGTCAAGGACGTGCTGCAGGAGATGATCGC
CCAGATCCAGGCGGGCGAGGCCAAGCCGAACAAGACGGCGCTCGCCAAGTGGTGGGAGCAGATCGAGCAGTGGCGCAGCG
TCGACTGCCTGAAGTACGACCGCACCTCCGAGATCATCAAGCCGCAGTACGTGGTCGAGAAGATCTGGGAACTGACCGCG
GGCGATGCCTTCGTCTGTTCCGACGTGGGCCAGCACCAGATGTGGGCCGCGCAGTTCTACAAGTTCAACGAGCCGCGCCG
CTGGATCAACTCCGGCGGTCTGGGCACGATGGGCGTGGGCCTGCCGTATGCCATGGGCGTCAAGAAGGCGTTCCCGGACA
AGGAGGTCGTCACCATCACCGGTGAGGGCTCGATCCAGATGTGCATCCAGGAACTCTCCACCTGTCTGCAGTACGACACG
CCGGTGAAGATCTGCTCGCTCAACAACGGCTACCTGGGCATGGTGCGCCAGTGGCAGGAGATCGAGTACGACAACCGTTA
TTCGCACTCCTACATGCAGGCGCTGCCTGACTTCGTGAAGCTGGCCGAAGCGTATGGCCACGTGGGCATGCGGATCGAAA
AGACCGCCGATGTCGAGCCCGCGCTGCGCGAAGCCTTCCGACTCAAGGACCGCACCGTGTTCCTCGACTTCCAGACCGAC
CCGACCGAAAACGTCTGGCCGATGGTCCAGGCAGGCAAGGGCATCTCTGAAATGCTGCTCGGCGCGGAGGATCTGTAA

Upstream 100 bases:

>100_bases
CAGGCGGCCATCCATGCAGACGCGCACCGCTCGAACCCGCGCCACAAGCCCGGTAGAGAGCATCCAAACAATTTTTTGCC
GAAAATTGCAAAGGACTGAC

Downstream 100 bases:

>100_bases
TGCGCCACATCATTTCCGTCCTGCTGGAAAACGAACCGGGCGCGCTGTCGCGCGTGGTGGGCCTGTTCTCGGCGCGCGGC
TACAACATCGAGACGCTGAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MNMPSAEFSHANSGSPAEMMGSDILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIASTGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
QLAALTGYPVTNTLMGLGAFPGASKQFLGMLGMHGTYEANMAMQHCDVLIAVGARFDDRVIGSPAHFASQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTA
GDAFVCSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKTADVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL

Sequences:

>Translated_585_residues
MNMPSAEFSHANSGSPAEMMGSDILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIASTGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
QLAALTGYPVTNTLMGLGAFPGASKQFLGMLGMHGTYEANMAMQHCDVLIAVGARFDDRVIGSPAHFASQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTA
GDAFVCSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKTADVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL
>Mature_585_residues
MNMPSAEFSHANSGSPAEMMGSDILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIASTGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
QLAALTGYPVTNTLMGLGAFPGASKQFLGMLGMHGTYEANMAMQHCDVLIAVGARFDDRVIGSPAHFASQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTA
GDAFVCSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKTADVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=581, Percent_Identity=25.3012048192771, Blast_Score=173, Evalue=5e-43,
Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=26.3671875, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=56.6901408450704, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=39.2294220665499, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=34.3807763401109, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=28.4955752212389, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=25.2252252252252, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=552, Percent_Identity=25.9057971014493, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=552, Percent_Identity=25.9057971014493, Blast_Score=158, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=511, Percent_Identity=26.027397260274, Blast_Score=151, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.7101200686106, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.040404040404, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320123, Length=511, Percent_Identity=21.3307240704501, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=532, Percent_Identity=21.9924812030075, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25.6993006993007, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64383; Mature: 64383

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSHANSGSPAEMMGSDILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEH
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHHCCCHHH
ILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPT
HEEECHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
HAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKAFYIASTGRPGPVVVDIPKDV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHH
SRELCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
HHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QLAALTGYPVTNTLMGLGAFPGASKQFLGMLGMHGTYEANMAMQHCDVLIAVGARFDDRV
HHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCC
IGSPAHFASQPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKTA
CCCCHHHHCCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHH
LAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTAGDAFVCSDVGQHQMWAAQFY
HHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
KFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKTADVEP
CEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH
ALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSHANSGSPAEMMGSDILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEH
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHHCCCHHH
ILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPT
HEEECHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
HAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKAFYIASTGRPGPVVVDIPKDV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHH
SRELCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
HHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QLAALTGYPVTNTLMGLGAFPGASKQFLGMLGMHGTYEANMAMQHCDVLIAVGARFDDRV
HHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCC
IGSPAHFASQPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKTA
CCCCHHHHCCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHH
LAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTAGDAFVCSDVGQHQMWAAQFY
HHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
KFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKTADVEP
CEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH
ALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]