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The map label for this gene is pnp [H]

Identifier: 17546786

GI number: 17546786

Start: 2239684

End: 2241837

Strand: Reverse

Name: pnp [H]

Synonym: RSc2067

Alternate gene names: 17546786

Gene position: 2241837-2239684 (Counterclockwise)

Preceding gene: 17546787

Following gene: 17546785

Centisome position: 60.32

GC content: 65.65

Gene sequence:

>2154_bases
ATGTTCAACAAGATCGTCAAGGAATTCCAGTGGGGCAACCACAAGGTGCGCATGGAGACGGGCGAGATCGCTCGCCAGGC
CAGCGGCGCCGTGCTGCTCGACATGGACGACACCGTGGTGCTGGCCACCGTGGTGGGCGCCAAGAACGCCAAGCCGGGCC
AGGATTTCTTCCCGCTGACCGTCGACTACATCGAGAAGACCTACGCCGCCGGCAAGATCCCGGGTGGCTTCTTCAAGCGT
GAAGGCCGTCCGTCGGAAAACGAGACGCTGACCTCGCGCCTGATCGACCGTCCGCTGCGTCCGCTGTTCCCGGAAGGCTT
CTACAACGAAGTCCAGGTCGTCATCCACGTGCTGTCGATCAACCCGGAAGTGCCGGCCGACATCCCGGCGCTGGTGGCCG
CTTCGGCCGCGCTGGCCGTGTCGGGCCTGCCGTTCAACGGCCCGGTGGGCGCCGCCCGCGTGGGTTACAAGGACGGCCAG
TACCTGCTGAACCCGAACCGCGCGCAACTGGCGCATTCGGACCTGGACCTGGTGGTTGCCGGCACCGAGCGTGCCGTGCT
GATGGTCGAATCCGAAGCCAACCAGCTGTCGGAAGAGGTGATGCTGGGCGCCGTGGTGTACGGCCATGAGCAGATGCAGA
TCGCCATCAATGCGATCCACGACCTGGTGCGCGACGGCGGCAAGCCGGAGTGGGACTGGCAAGCGGCGCCGAAGAACGAA
GCCCTGGTCGCCAAGGTGTCCGAGCTGGGCTTGGCCGATCTGCAAGCCGCTTACCAGCTGCGCCAGAAGTCGGCCCGCAG
CCAGAAGCTGAAGGAAGTCTACGCATCGGTCGCGGCCAAGCTGGCCGAAGCCGGCGTGGAAGCCGACGGCGTGGAAGTCG
ACAACATCCTGTTCGAGCTCGAATCGAAGATCGTGCGCGGCCAGATCCTGAACGGCGAGCCGCGTATCGACGGTCGCGAC
ACCCGCACGGTGCGCCCGATCGAGATCCGCTCGTCGGTGCTGCCGCGCGCGCACGGCTCGGCGCTGTTCACGCGCGGTGA
GACGCAAGCGCTGGTGGTGGCCACCCTGGGCACCAAGAGCGACGAGCAGATCATCGACGCGCTGCAGGGCGAGTACCGCG
ACCGCTTCATGCTCCACTACAACATGCCGCCGTTCGCCACCGGCGAAACCGGCCGCGTGGGCAGCCCGAAGCGCCGCGAA
ATCGGCCACGGCCGCCTGGCCAAGCGCGCGCTGATCCCGGTGCTGCCGAAGGAAGACGAATTCGCCTACACGATTCGTCT
GGTGTCGGAGATCACCGAGTCGAACGGCTCGTCGTCGATGGCTTCGGTCTGCGGTGGCTGCCTGGCGCTGATGGATGCCG
GCGTGCCGGTCAAGGCGCACGTGGCCGGCGTGGCCATGGGCCTGATCCTGGAGGGCAACAAGTTCGCCGTGCTGACCGAC
ATCCTGGGCGACGAAGATCACCTGGGTGACATGGACTTCAAGGTGGCCGGTACCGATAACGGCATCACCGCGCTGCAGAT
GGACATCAAGGTGCAGGGCATCACCAAGGAAATCATGCAGGTCGCTCTGGCGCAGGCCAAGGAAGGCCGTCTGCATATCC
TGGGCAAGATGCAGGCCGCGATGGGCCATGCACGCACCGAACTGTCGGAGCATGCGCCGCGCATGATCACCGTCAAGATC
AATCCGGAGAAGATCCGCGACGTGATCGGCAAGGGCGGCTCGACCATCCAGGCGCTGACCAAGGAAACCGGCTGCACGAT
CGACATCCAGGAAGACGGCACGATCACCATCGCGTCGACCTCGTCCGAGGGCATGGCCGAAGCCAAGCGCCGCATCGAAG
GCATCACGGCCGAAGCCGAAGTGGGCAAGATCTACAGCGGCACCGTGCTCAAGCTGCTGGACTTCGGTGCGATCGTCAAC
ATCCTGCCGGGCAAGGACGGCCTGCTGCACATCTCGGAGATCGCCAACGAGCGCGTCAACCAGGTGTCGGACTACGTCAA
GGAAGGCCAGATGGTGCGCGTGAAGCTGCTGAGCACCGACGAAAAGGGCCGCATGCGCCTGTCGATCAAGGCCGCCAAGG
CTGAGGAAGGCGATGTGCCGGCCACCGCGCCGCAGGCGCCGGGCGCCGGCGACGCGACGTCGCAGCAGCAGTAA

Upstream 100 bases:

>100_bases
GAATGGCATAAACACACCTCTGCAACCGCACCGTCTTCCGGAGCGTGTTGGTCGCGGCGCAAGCAACGATGCGCGCCGCG
CATACCGAACAGGAGAAGAC

Downstream 100 bases:

>100_bases
GCGCGGGAATCTCCGCCACGGAAACGGCTGGACAGTGTCCAGCCGTTTTTTTGTTTACACTGCGTCGCGGGTTCCACCAA
CGCGACCGACCATGAGCCGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MFNKIVKEFQWGNHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYAAGKIPGGFFKR
EGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQ
YLLNPNRAQLAHSDLDLVVAGTERAVLMVESEANQLSEEVMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKNE
ALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYASVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDGRD
TRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALIPVLPKEDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLTD
ILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGHARTELSEHAPRMITVKI
NPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYSGTVLKLLDFGAIVN
ILPGKDGLLHISEIANERVNQVSDYVKEGQMVRVKLLSTDEKGRMRLSIKAAKAEEGDVPATAPQAPGAGDATSQQQ

Sequences:

>Translated_717_residues
MFNKIVKEFQWGNHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYAAGKIPGGFFKR
EGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQ
YLLNPNRAQLAHSDLDLVVAGTERAVLMVESEANQLSEEVMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKNE
ALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYASVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDGRD
TRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALIPVLPKEDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLTD
ILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGHARTELSEHAPRMITVKI
NPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYSGTVLKLLDFGAIVN
ILPGKDGLLHISEIANERVNQVSDYVKEGQMVRVKLLSTDEKGRMRLSIKAAKAEEGDVPATAPQAPGAGDATSQQQ
>Mature_717_residues
MFNKIVKEFQWGNHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYAAGKIPGGFFKR
EGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQ
YLLNPNRAQLAHSDLDLVVAGTERAVLMVESEANQLSEEVMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKNE
ALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYASVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDGRD
TRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALIPVLPKEDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLTD
ILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGHARTELSEHAPRMITVKI
NPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYSGTVLKLLDFGAIVN
ILPGKDGLLHISEIANERVNQVSDYVKEGQMVRVKLLSTDEKGRMRLSIKAAKAEEGDVPATAPQAPGAGDATSQQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=37.7437325905292, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=62.7167630057804, Blast_Score=899, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=725, Percent_Identity=32.2758620689655, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI281362905, Length=727, Percent_Identity=36.8638239339752, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=727, Percent_Identity=36.8638239339752, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=727, Percent_Identity=36.8638239339752, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=667, Percent_Identity=36.7316341829085, Blast_Score=397, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77194; Mature: 77194

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKIVKEFQWGNHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNAKPGQDFFPLT
CHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCC
VDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSI
HHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEEC
NPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQYLLNPNRAQLAHSDLDLVVA
CCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCEEEEE
GTERAVLMVESEANQLSEEVMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKNE
CCCEEEEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYASVAAKLAEAGVEADGVEVDNILFEL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ESKIVRGQILNGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKS
HHHHHCCEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCC
DEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKEDE
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCCCC
FAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLTD
CEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEEEE
ILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAA
CCCCCCCCCCCEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHHH
MGHARTELSEHAPRMITVKINPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIAST
HHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCEEEEEEC
SSEGMAEAKRRIEGITAEAEVGKIYSGTVLKLLDFGAIVNILPGKDGLLHISEIANERVN
CCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
QVSDYVKEGQMVRVKLLSTDEKGRMRLSIKAAKAEEGDVPATAPQAPGAGDATSQQQ
HHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFNKIVKEFQWGNHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNAKPGQDFFPLT
CHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCC
VDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSI
HHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEEC
NPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQYLLNPNRAQLAHSDLDLVVA
CCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCEEEEE
GTERAVLMVESEANQLSEEVMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKNE
CCCEEEEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYASVAAKLAEAGVEADGVEVDNILFEL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ESKIVRGQILNGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKS
HHHHHCCEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCC
DEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKEDE
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCCCC
FAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLTD
CEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEEEE
ILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAA
CCCCCCCCCCCEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHHH
MGHARTELSEHAPRMITVKINPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIAST
HHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCEEEEEEC
SSEGMAEAKRRIEGITAEAEVGKIYSGTVLKLLDFGAIVNILPGKDGLLHISEIANERVN
CCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
QVSDYVKEGQMVRVKLLSTDEKGRMRLSIKAAKAEEGDVPATAPQAPGAGDATSQQQ
HHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA