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The map label for this gene is tpiA

Identifier: 17546783

GI number: 17546783

Start: 2236772

End: 2237518

Strand: Reverse

Name: tpiA

Synonym: RSc2064

Alternate gene names: 17546783

Gene position: 2237518-2236772 (Counterclockwise)

Preceding gene: 17546784

Following gene: 17546782

Centisome position: 60.21

GC content: 68.27

Gene sequence:

>747_bases
GTGAGCGCAAGACCGAAGCTGGTGGTGGGCAACTGGAAGTTGCATGGCAGCCTGAACGGCAACGCCGAGCTGCTTGAGAA
GATCAAGGCAGCCGGGCAGACCCGCGCCGCGCTGGCCGTCTGTGCACCGTTCCCGTACCTGGCCCAGTGCCAGTCGGTGC
TCGCCGGCTCGTCGGTGGCCTGGGGCGCGCAGGACGTGTCGGCCGAGACGCGCGGCGCCTTCACCGGCGAGGTGGCGGCG
TCGATGTTGTCGGAATTTGGCTGCGGCTACGCGATCGTCGGGCACTCGGAACGTCGCACGTATCACGGCGAGACCGACGC
GCAGGTGGCGATCAAGGCGCTGCGCGCGCTGGAGCACGGCATCACCCCGATCGTCTGTGTCGGCGAAACGCTGGCGCAAC
GCGAGGCCGGAGAGACCGAGCTAGTCGTGGCCCGTCAGTTGGAGGCGGTGCTGGAGTCGCTGTCGGTCGAACAGCTGGGT
CACATCGTCGTGGCCTACGAGCCGGTCTGGGCCATCGGTACCGGCAAGACCGCGACCAGCGAGCAGGCGCAGGCAGTGCA
CGCGTTCCTGCGTGGCCGCGTGGCTGCTTGCGATGCCGGTGTCGCGCAGCGCATGCCGATCCTGTACGGCGGCAGCGTCA
AGCCGGACAACGCGGCCGAACTGTTTACGATGGCAGACATCGATGGCGGCCTGATCGGCGGCGCATCCCTGAAGGCCGAA
GATTTCCTGGCGATCGGCCGGGCCTGA

Upstream 100 bases:

>100_bases
GCACGCATGCGGTCGCGTCCGGGCGACAAAATTGACCCCGTTTTTGCCGCCACGGTAGAATGCCGGGTTTATTTGACTGT
CGACTTGGCGGAACAACAAC

Downstream 100 bases:

>100_bases
TCGGCCCCCGAGATCGCCGTCCGTTGTGGCTCCTGGGGTCACAACACATTATTGAGAGAAGCGAAATGGCAATTTTGAAG
ACTCTGCTGCTGGTGGCGCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSARPKLVVGNWKLHGSLNGNAELLEKIKAAGQTRAALAVCAPFPYLAQCQSVLAGSSVAWGAQDVSAETRGAFTGEVAA
SMLSEFGCGYAIVGHSERRTYHGETDAQVAIKALRALEHGITPIVCVGETLAQREAGETELVVARQLEAVLESLSVEQLG
HIVVAYEPVWAIGTGKTATSEQAQAVHAFLRGRVAACDAGVAQRMPILYGGSVKPDNAAELFTMADIDGGLIGGASLKAE
DFLAIGRA

Sequences:

>Translated_248_residues
MSARPKLVVGNWKLHGSLNGNAELLEKIKAAGQTRAALAVCAPFPYLAQCQSVLAGSSVAWGAQDVSAETRGAFTGEVAA
SMLSEFGCGYAIVGHSERRTYHGETDAQVAIKALRALEHGITPIVCVGETLAQREAGETELVVARQLEAVLESLSVEQLG
HIVVAYEPVWAIGTGKTATSEQAQAVHAFLRGRVAACDAGVAQRMPILYGGSVKPDNAAELFTMADIDGGLIGGASLKAE
DFLAIGRA
>Mature_247_residues
SARPKLVVGNWKLHGSLNGNAELLEKIKAAGQTRAALAVCAPFPYLAQCQSVLAGSSVAWGAQDVSAETRGAFTGEVAAS
MLSEFGCGYAIVGHSERRTYHGETDAQVAIKALRALEHGITPIVCVGETLAQREAGETELVVARQLEAVLESLSVEQLGH
IVVAYEPVWAIGTGKTATSEQAQAVHAFLRGRVAACDAGVAQRMPILYGGSVKPDNAAELFTMADIDGGLIGGASLKAED
FLAIGRA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=239, Percent_Identity=42.2594142259414, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI226529917, Length=239, Percent_Identity=42.2594142259414, Blast_Score=171, Evalue=5e-43,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=50.2024291497976, Blast_Score=236, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=48.207171314741, Blast_Score=193, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6320255, Length=240, Percent_Identity=43.75, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=44.1666666666667, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=44.1666666666667, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28572004, Length=240, Percent_Identity=44.1666666666667, Blast_Score=181, Evalue=3e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_RALSO (Q8XXP9)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520185.1
- ProteinModelPortal:   Q8XXP9
- SMR:   Q8XXP9
- GeneID:   1220905
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2064
- NMPDR:   fig|267608.1.peg.2064
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   RSOL267608:RSC2064-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25830; Mature: 25698

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 11-11 BINDING 13-13

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARPKLVVGNWKLHGSLNGNAELLEKIKAAGQTRAALAVCAPFPYLAQCQSVLAGSSVA
CCCCCCEEEECEEEEECCCCCHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHCCCCCC
WGAQDVSAETRGAFTGEVAASMLSEFGCGYAIVGHSERRTYHGETDAQVAIKALRALEHG
CCCHHCCCHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
ITPIVCVGETLAQREAGETELVVARQLEAVLESLSVEQLGHIVVAYEPVWAIGTGKTATS
CCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCCCH
EQAQAVHAFLRGRVAACDAGVAQRMPILYGGSVKPDNAAELFTMADIDGGLIGGASLKAE
HHHHHHHHHHHCCHHHCCCCHHHCCCEEECCCCCCCCHHHEEEEECCCCCEECCCCCCHH
DFLAIGRA
HHHEECCC
>Mature Secondary Structure 
SARPKLVVGNWKLHGSLNGNAELLEKIKAAGQTRAALAVCAPFPYLAQCQSVLAGSSVA
CCCCCEEEECEEEEECCCCCHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHCCCCCC
WGAQDVSAETRGAFTGEVAASMLSEFGCGYAIVGHSERRTYHGETDAQVAIKALRALEHG
CCCHHCCCHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
ITPIVCVGETLAQREAGETELVVARQLEAVLESLSVEQLGHIVVAYEPVWAIGTGKTATS
CCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCCCH
EQAQAVHAFLRGRVAACDAGVAQRMPILYGGSVKPDNAAELFTMADIDGGLIGGASLKAE
HHHHHHHHHHHCCHHHCCCCHHHCCCEEECCCCCCCCHHHEEEEECCCCCEECCCCCCHH
DFLAIGRA
HHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852