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The map label for this gene is prpD [H]

Identifier: 17546721

GI number: 17546721

Start: 2178619

End: 2180085

Strand: Direct

Name: prpD [H]

Synonym: RSc2002

Alternate gene names: 17546721

Gene position: 2178619-2180085 (Clockwise)

Preceding gene: 17546720

Following gene: 17546722

Centisome position: 58.62

GC content: 65.64

Gene sequence:

>1467_bases
ATGTCCGCGCCGGCTGCGAACATCCGCCCGAAACCCGACAAGGTCATCGTCGACATCGTCGACTACGTGACCCGGTTCAA
GGTCGACAGCAAGGCCGCCTATGAAACGGCGCGCCACTGCCTGATCGACACCTTGGGCTGCGGGCTGGAGGCGCTGTCGT
ATCCGGCGTGCACCAAGCTCCTGGGGCCCATCGTGCCGGGAACCGTCGTGCCGAACGGCGCGAAGGTCCCGGGCACCCAA
TTCCAGCTCGATCCGGTGCAGGCGGCGTTCAACATCGGCGCCATGATCCGCTGGCTGGATTTCAACGACACGTGGCTGGC
CGCCGAATGGGGCCATCCGTCGGACAACCTCGGCGGCATCCTGGCCGTCGCGGACTGGCTGTCCCGCACGAACCTCGCCG
GCGGCAAGAAGCCGCTCGCCATGAGGGACGTGCTGACCGCGATGATCAAGGCCCACGAGATCCAGGGCTGCATCGCGCTC
GAAAACGCATTCAACCAGGTGGGGCTGGACCACGTCGTGCTGGTCAAGGTCGCCACCGCCGCGGTGGTCGGGCAACTGCT
CGGGCTGTCGCGCGAGGCGCTCATCAACGCCGTGTCGCTGGCGTGGGTCGATGGCCAGGCGCTGCGCACCTACCGCCATG
CCCCGAACACGGGTAGCCGCAAGTCCTGGGCGGCGGGCGACGCCACCAGCCGCGGCGTGCGCCTGGCGCTGATCGCCAGG
ACCGGCGAGATGGGCTATCCCTCCGCCCTCACCGCCAAGGGCTGGGGCTTCTATGACGTGTCCTTCAAGGGCCGGCCGTT
CAGGTTCCAGCGCAAGTACGGCACGTACGTGATGGAAAACGTGCTGTTCAAGATCAGCTTCCCGGCCGAGTTCCATGCGC
AGACCGCCGCCGAAGCGGCCATGACACTGCACAGGGCCATTGCCAAGGCCGGCAAGACCGTCGATGACATCGAGCAGATC
ACAATCCGCACGCACGCAGCGGCAATCCGCATCATCGACAAGCAAGGCCCGCTGAACAATCCGGCGGACCGCGACCATTG
CATCCAGTACATGGTCGCCGTGCCGCTCATCTTCGGCCGCCTGACCGCCGGGGATTACGAAGACACCGTCGCCCGCGACC
CGCGCATCGATGCGCTGCGCAAGAAGATCGTCTGCGTGGAAGACCCGCAGTTCACCAAGGATTACCACGACCCGGAGAAG
CGTTCGATCGCCAATGGGATCACCGTCCGGTTCAAGGGCAAGACGGCGCCCAACGAAAGGGCGCTCAAGGAGGTCGTGGT
GGAGTACCCGATCGGCCACAGGCGGCGGCGCAAGGAAGGCATGCCGCTGCTGCTCGAGAAGTTCAGGACCAACCTGGCCC
GCCGCTTTCCGGCCAGGCAGCAGGACGCCATCCTGCGCGCTTCGCTGGACCAGAAAGCACTCGAGGCCATGCCGGTCAAC
GAGTATGTCGATTTGTACGTGATTTGA

Upstream 100 bases:

>100_bases
GATGATGCAGGACAACGCCGCACCGGCGCCCGCTCCGGCCCCCACGCCGGACGCCACGCAACCGGCCAAGCCGTAACGCG
AGAGGCCCCGGAAACACGCC

Downstream 100 bases:

>100_bases
TTTGATGTATCCGCGCGGGGTGCTCCTGGTGCTCCGCGCATCCTTGAGCCTTGGAGTCATGCCATGCCCCACAACCTGAA
AAAGACACTCAAAACATTCA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSAPAANIRPKPDKVIVDIVDYVTRFKVDSKAAYETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTVVPNGAKVPGTQ
FQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAVADWLSRTNLAGGKKPLAMRDVLTAMIKAHEIQGCIAL
ENAFNQVGLDHVVLVKVATAAVVGQLLGLSREALINAVSLAWVDGQALRTYRHAPNTGSRKSWAAGDATSRGVRLALIAR
TGEMGYPSALTAKGWGFYDVSFKGRPFRFQRKYGTYVMENVLFKISFPAEFHAQTAAEAAMTLHRAIAKAGKTVDDIEQI
TIRTHAAAIRIIDKQGPLNNPADRDHCIQYMVAVPLIFGRLTAGDYEDTVARDPRIDALRKKIVCVEDPQFTKDYHDPEK
RSIANGITVRFKGKTAPNERALKEVVVEYPIGHRRRRKEGMPLLLEKFRTNLARRFPARQQDAILRASLDQKALEAMPVN
EYVDLYVI

Sequences:

>Translated_488_residues
MSAPAANIRPKPDKVIVDIVDYVTRFKVDSKAAYETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTVVPNGAKVPGTQ
FQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAVADWLSRTNLAGGKKPLAMRDVLTAMIKAHEIQGCIAL
ENAFNQVGLDHVVLVKVATAAVVGQLLGLSREALINAVSLAWVDGQALRTYRHAPNTGSRKSWAAGDATSRGVRLALIAR
TGEMGYPSALTAKGWGFYDVSFKGRPFRFQRKYGTYVMENVLFKISFPAEFHAQTAAEAAMTLHRAIAKAGKTVDDIEQI
TIRTHAAAIRIIDKQGPLNNPADRDHCIQYMVAVPLIFGRLTAGDYEDTVARDPRIDALRKKIVCVEDPQFTKDYHDPEK
RSIANGITVRFKGKTAPNERALKEVVVEYPIGHRRRRKEGMPLLLEKFRTNLARRFPARQQDAILRASLDQKALEAMPVN
EYVDLYVI
>Mature_487_residues
SAPAANIRPKPDKVIVDIVDYVTRFKVDSKAAYETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTVVPNGAKVPGTQF
QLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAVADWLSRTNLAGGKKPLAMRDVLTAMIKAHEIQGCIALE
NAFNQVGLDHVVLVKVATAAVVGQLLGLSREALINAVSLAWVDGQALRTYRHAPNTGSRKSWAAGDATSRGVRLALIART
GEMGYPSALTAKGWGFYDVSFKGRPFRFQRKYGTYVMENVLFKISFPAEFHAQTAAEAAMTLHRAIAKAGKTVDDIEQIT
IRTHAAAIRIIDKQGPLNNPADRDHCIQYMVAVPLIFGRLTAGDYEDTVARDPRIDALRKKIVCVEDPQFTKDYHDPEKR
SIANGITVRFKGKTAPNERALKEVVVEYPIGHRRRRKEGMPLLLEKFRTNLARRFPARQQDAILRASLDQKALEAMPVNE
YVDLYVI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=488, Percent_Identity=76.4344262295082, Blast_Score=794, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=488, Percent_Identity=60.655737704918, Blast_Score=597, Evalue=1e-171,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53807; Mature: 53676

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAANIRPKPDKVIVDIVDYVTRFKVDSKAAYETARHCLIDTLGCGLEALSYPACTKL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
LGPIVPGTVVPNGAKVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCHH
LAVADWLSRTNLAGGKKPLAMRDVLTAMIKAHEIQGCIALENAFNQVGLDHVVLVKVATA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
AVVGQLLGLSREALINAVSLAWVDGQALRTYRHAPNTGSRKSWAAGDATSRGVRLALIAR
HHHHHHHCCCHHHHHHHHHHEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEE
TGEMGYPSALTAKGWGFYDVSFKGRPFRFQRKYGTYVMENVLFKISFPAEFHAQTAAEAA
CCCCCCCCCEEECCCCEEEECCCCCCEEEHHHHHHHHHHCEEEEEECCHHHHHHHHHHHH
MTLHRAIAKAGKTVDDIEQITIRTHAAAIRIIDKQGPLNNPADRDHCIQYMVAVPLIFGR
HHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAGDYEDTVARDPRIDALRKKIVCVEDPQFTKDYHDPEKRSIANGITVRFKGKTAPNER
CCCCCCHHHHCCCCCHHHHHHCEEEECCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHH
ALKEVVVEYPIGHRRRRKEGMPLLLEKFRTNLARRFPARQQDAILRASLDQKALEAMPVN
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
EYVDLYVI
CCEEEEEC
>Mature Secondary Structure 
SAPAANIRPKPDKVIVDIVDYVTRFKVDSKAAYETARHCLIDTLGCGLEALSYPACTKL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
LGPIVPGTVVPNGAKVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCHH
LAVADWLSRTNLAGGKKPLAMRDVLTAMIKAHEIQGCIALENAFNQVGLDHVVLVKVATA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
AVVGQLLGLSREALINAVSLAWVDGQALRTYRHAPNTGSRKSWAAGDATSRGVRLALIAR
HHHHHHHCCCHHHHHHHHHHEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEE
TGEMGYPSALTAKGWGFYDVSFKGRPFRFQRKYGTYVMENVLFKISFPAEFHAQTAAEAA
CCCCCCCCCEEECCCCEEEECCCCCCEEEHHHHHHHHHHCEEEEEECCHHHHHHHHHHHH
MTLHRAIAKAGKTVDDIEQITIRTHAAAIRIIDKQGPLNNPADRDHCIQYMVAVPLIFGR
HHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAGDYEDTVARDPRIDALRKKIVCVEDPQFTKDYHDPEKRSIANGITVRFKGKTAPNER
CCCCCCHHHHCCCCCHHHHHHCEEEECCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHH
ALKEVVVEYPIGHRRRRKEGMPLLLEKFRTNLARRFPARQQDAILRASLDQKALEAMPVN
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
EYVDLYVI
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]