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The map label for this gene is truA [H]

Identifier: 17546704

GI number: 17546704

Start: 2159323

End: 2160147

Strand: Reverse

Name: truA [H]

Synonym: RSc1985

Alternate gene names: 17546704

Gene position: 2160147-2159323 (Counterclockwise)

Preceding gene: 17546705

Following gene: 17546703

Centisome position: 58.12

GC content: 68.73

Gene sequence:

>825_bases
ATGACGCGCATCGCGCTCGGCATCCAGTACGACGGTGCCGCGTTTTCGGGATGGCAGTCGCAACCGCACGGCAACACCGT
GCAGGACGTGCTCGAGGCGGCGCTGCGCGAGTTTGCCGGCGTGGCCTTGCCGACCACCGTGGCAGGCCGCACCGACGCCG
GCGTGCATGCGCTGGGGCAGGTGGTCCATCTCGACACCGAGCTGGTGCGCGATCCATTCTCGTGGGTGCGCGGCACCAAT
GCCTTCCTCCCGCCGACGGTAGCCGTGCGCTGGGCGCAGGCGATGCCGGAAGATTTTCACGCGCGCTTCTCCGCGCATCG
CCGCACGTACTACTACGCGCTGACCTACGGTCCGACGCGCGCACCGCTGCTCGAAGGCAAGGCCGGCTACGTGACGCTGC
CGCCCGGACAGTCACTTGACGTGACAGCCATGCACGAGGCCGCGCAGGTGCTGGTCGGCGAGCACGATTTTTCCTCCTTC
CGTGCGGCAGAATGCCAGGCCAAGTCGCCCGTCAAGACGATGTATTCGACCGAGGTGCGGGGGCAGGGCGAATGGGTGTT
CGTGCGCATCCGCGGCAGCGCCTTCCTGCATCACATGGTGCGCAACATCATGGGCTGCCTGGTGGCGATCGGCCGGGGCA
AGCGCCCGGCGGCGTGGATGGGCGAGGTGCTGGCCGCGCGCGACCGCAAGGCCGCCGCCCCCACCTTCATGCCCGACGGC
CTGTACCTTGCCGAGGTCGGCTATCCTGATGCCTTCCGGCTGCCGGCATCTCCGGCGTCGTCTAGCCTGTTTCGCGGCGT
GTTCGACGAGCACGCCGGCCCATGA

Upstream 100 bases:

>100_bases
CGCCAGATCGGCTGATCGGCACTGCGCCGTACGGCTTCGCCAACGCCGGCAGGGTGGTATCCTGCCGGCGTTTTTCATTT
CGCATGGGGATTCATTCGGC

Downstream 100 bases:

>100_bases
CCATTGCCATGCCGCTTCACCGTACCCGTATCAAGCTCTGTGGCCTGACCCAGCCGGACGACGTCGACCATGCCGTCGCG
CTGGGCGCCGATGCCATCGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTRIALGIQYDGAAFSGWQSQPHGNTVQDVLEAALREFAGVALPTTVAGRTDAGVHALGQVVHLDTELVRDPFSWVRGTN
AFLPPTVAVRWAQAMPEDFHARFSAHRRTYYYALTYGPTRAPLLEGKAGYVTLPPGQSLDVTAMHEAAQVLVGEHDFSSF
RAAECQAKSPVKTMYSTEVRGQGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGKRPAAWMGEVLAARDRKAAAPTFMPDG
LYLAEVGYPDAFRLPASPASSSLFRGVFDEHAGP

Sequences:

>Translated_274_residues
MTRIALGIQYDGAAFSGWQSQPHGNTVQDVLEAALREFAGVALPTTVAGRTDAGVHALGQVVHLDTELVRDPFSWVRGTN
AFLPPTVAVRWAQAMPEDFHARFSAHRRTYYYALTYGPTRAPLLEGKAGYVTLPPGQSLDVTAMHEAAQVLVGEHDFSSF
RAAECQAKSPVKTMYSTEVRGQGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGKRPAAWMGEVLAARDRKAAAPTFMPDG
LYLAEVGYPDAFRLPASPASSSLFRGVFDEHAGP
>Mature_273_residues
TRIALGIQYDGAAFSGWQSQPHGNTVQDVLEAALREFAGVALPTTVAGRTDAGVHALGQVVHLDTELVRDPFSWVRGTNA
FLPPTVAVRWAQAMPEDFHARFSAHRRTYYYALTYGPTRAPLLEGKAGYVTLPPGQSLDVTAMHEAAQVLVGEHDFSSFR
AAECQAKSPVKTMYSTEVRGQGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGKRPAAWMGEVLAARDRKAAAPTFMPDGL
YLAEVGYPDAFRLPASPASSSLFRGVFDEHAGP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=33.2116788321168, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI199559785, Length=264, Percent_Identity=29.9242424242424, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788657, Length=256, Percent_Identity=46.09375, Blast_Score=236, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI71985046, Length=267, Percent_Identity=27.3408239700375, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29814; Mature: 29683

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIALGIQYDGAAFSGWQSQPHGNTVQDVLEAALREFAGVALPTTVAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHH
VVHLDTELVRDPFSWVRGTNAFLPPTVAVRWAQAMPEDFHARFSAHRRTYYYALTYGPTR
HHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEECCCC
APLLEGKAGYVTLPPGQSLDVTAMHEAAQVLVGEHDFSSFRAAECQAKSPVKTMYSTEVR
CCEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCC
GQGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGKRPAAWMGEVLAARDRKAAAPTFMPDG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLAEVGYPDAFRLPASPASSSLFRGVFDEHAGP
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRIALGIQYDGAAFSGWQSQPHGNTVQDVLEAALREFAGVALPTTVAGRTDAGVHALGQ
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHH
VVHLDTELVRDPFSWVRGTNAFLPPTVAVRWAQAMPEDFHARFSAHRRTYYYALTYGPTR
HHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEECCCC
APLLEGKAGYVTLPPGQSLDVTAMHEAAQVLVGEHDFSSFRAAECQAKSPVKTMYSTEVR
CCEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCC
GQGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGKRPAAWMGEVLAARDRKAAAPTFMPDG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLAEVGYPDAFRLPASPASSSLFRGVFDEHAGP
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA